Molecular Analysis of Rheum Species Used as Rhei Rhizoma Based on the Chloroplast matK Gene Sequence and Its Application for Identification

AIM: The main goal of the present study was to find azole-resistant and molecular analysis of cyp51A gene in Aspergillus fumigatus.MATERIALS AND METHODS: Fifty-eight A. fumigatus strains including environmental, clinical and reference isolates were assessed in this investigation. Azole susceptibility testing for itraconazole and voriconazole was carried out for A. fumigatus isolates. PCR was performed based on cyp51A gene sequence for all isolates.RESULTS: Susceptibility testing verified the minimum inhibitory concentrations (MICs) for itraconazole (0.125 to 2 µg/ml) and voriconazole (0.125 to 4 µg/ml). Nine (15.5%) A. fumigatus isolates were resistant to voriconazole with MIC 4 µg/ml. A 1500 bp DNA fragment was amplified using cyp51A gene for all tested Aspergillus isolates. The sequences of the fragments showed 99% identity with A. fumigatus cyp51A gene in the GenBank. No point mutation was found at cyp51A gene codons.CONCLUSION: In the current study, we detected the voriconazile resistant in A. fumigatus isolates. Susceptibility tests should be considered in patients who infected by A. fumigatus.

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The matK gene: sequence variation and application in plant systematics

American Journal of Botany ◽

10.2307/2445819 ◽

1997 ◽

Vol 84 (6) ◽

pp. 830-839 ◽

Cited By ~ 132

Author(s):

Khidir W. Hilu ◽

gping Liang

Keyword(s):

Sequence Variation ◽

Gene Sequence ◽

Plant Systematics ◽

Matk Gene

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Molecular systematics of Lilium and allied genera (Liliaceae): phylogenetic relationships among Lilium and related genera based on the rbcL and matK gene sequence data

Plant Species Biology ◽

10.1046/j.1442-1984.2000.00025.x ◽

2000 ◽

Vol 15 (1) ◽

pp. 73-93 ◽

Cited By ~ 59

Author(s):

Kazuhiko Hayashi1 ◽

Shoichi Kawano2

Keyword(s):

Molecular Systematics ◽

Phylogenetic Relationships ◽

Gene Sequence ◽

Sequence Data ◽

Matk Gene ◽

Gene Sequence Data

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Molecular systematics of Lilium and allied genera (Liliaceae): phylogenetic relationships among Lilium and related genera based on the rbcL and matK gene sequence data

Plant Species Biology ◽

10.1046/j.1442-1984.2000.00025-2.x ◽

2000 ◽

Vol 15 (1) ◽

pp. 93-93

Keyword(s):

Molecular Systematics ◽

Phylogenetic Relationships ◽

Gene Sequence ◽

Sequence Data ◽

Matk Gene ◽

Gene Sequence Data

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Variasi Gen matK dan Filogenetik Tumbuhan Kantong Semar (Nepenthes sp.) dari Gunung Mahawu dan Gunung Soputan di Sulawesi Utara (The Variation of matK Gene and the Phylogeny of Nepenthes sp. Obtained from Mount Mahawu and Mount Soputan in North Sulawesi)

JURNAL BIOS LOGOS ◽

10.35799/jbl.7.1.2017.15816 ◽

2017 ◽

Vol 7 (1) ◽

Author(s):

Jenifer Tambuwun ◽

Beivy J Kolondam ◽

Trina E Tallei

Keyword(s):

Gene Sequence ◽

Forest Conversion ◽

Nucleotide Difference ◽

Pitcher Plant ◽

North Sulawesi ◽

As Species ◽

Matk Gene ◽

Base Analysis ◽

Protected Plants ◽

Barcode Gap

Abstrak Kantong semar (Nepenthes sp.) merupakan salah satu tumbuhan langka yang dilindungi. Eksploitasi yang berlebihan, serta alih fungsi hutan menjadi ancaman bagi kehidupan Nepenthes sp. Penelitian ini bertujuan untuk menentukan variasi gen matK dari tumbuhan Nepenthes sp. di Gunung Mahawu (JTM) dan Gunung Soputan (JTS) di Sulawesi Utara dan membandingkannya dengan kerabat terdekat di GenBank, serta membuat pohon filogenetiknya. Hasil penelitian menunjukkan bahwa hanya terdapat satu perbedaan nukleotida sekuens gen matK antara Nepenthes sp. dari Gunung Mahawu dan Gunung Soputan. Selain itu, variasi juga ditunjukan pada Nepenthes sp. yang diperoleh dari basis data GenBank dengan adanya perbedaan 1-7 basa nukleotida dengan sampel penelitian ini. Hasil analisis menggunakan ABGD (Automatic Barcode Gap Discovery) menunjukkan bahwa variasi intraspesies untuk Nepenthes sp. berada dalam rentang 0,000 – 0,004. Apabila mempertimbangkan barcode gap tersebut sebagai pembatas spesies, maka diasumsikan bahwa JTM, JTS, N. fusca, N. pilosa, N. maxima, N. faizaliana, dan N. clipeata merupakan spesies yang sama. Hal ini ditunjang oleh rekonstruksi pohon filogenetik menggunakan gen matK.Kata Kunci: ABGD, barcode gap, gen matK, Nepenthes sp., pohon filogenetik, variasi sekuens. Abstract Tropical pitcher plant (Nepenthes sp.) is listed as one of the endangered and protected plants. Excessive exploitation and forest conversion threaten the life of this Nepenthes sp. This research was aimed to determine variation in matK gene of Nepenthes sp. obtained from Mount Mahawu (JTM) and Mount Soputan (JTS) North Sulawesi, and compare the matK sequences with their allied taxa in public domain data base. Analysis of matK gene showed that there was only one nucleotide difference of matK gene sequence between Mahawu and Soputan samples. There were 1-7 different nucleotides between those samples and their allied taxa. Analysis of barcode gap using ABGD (Automatic Barcode Gap Discovery) showed that the range of intraspecies variation of Nepenthes sp. was 0,000 – 0,004. Considering this barcode gap generated from ABGD as species delimination, it could be assumed that JTM, JTS, N. fusca, N. pilosa, N. maxima, N. faizaliana, dan N. clipeata were the same species. These results were also supported by the reconstruction of phylogenetic tree using matK gene.Keywords: ABGD, barcode gap, matK gene, Nepenthes sp., phylogeneteic tree, sequence variation.

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Molecular Analysis of Coxiella Burnetii by Isocitrate Dehydrogenase Gene Sequence-Based Typing and PCR-RFLP in Isfahan, Iran.

Journal of World's Poultry Research ◽

10.36380/scil.2019.wvj8 ◽

2019 ◽

Vol 9 (2) ◽

pp. 52-58

Author(s):

Zary Nokhodian ◽

Mohammad Khalili ◽

Behrooz Ataei ◽

Awat Feizi ◽

Abdolreza Moradi ◽

...

Keyword(s):

Molecular Analysis ◽

Coxiella Burnetii ◽

Isocitrate Dehydrogenase ◽

Gene Sequence ◽

Dehydrogenase Gene ◽

Pcr Rflp

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Comparative Molecular Analysis of Endoevaporitic Microbial Communities

Applied and Environmental Microbiology ◽

10.1128/aem.00879-08 ◽

2008 ◽

Vol 74 (20) ◽

pp. 6444-6446 ◽

Cited By ~ 33

Author(s):

Jason W. Sahl ◽

Norman R. Pace ◽

John R. Spear

Keyword(s):

New Mexico ◽

Sequence Analysis ◽

Microbial Communities ◽

Molecular Analysis ◽

Gene Sequence ◽

Rrna Gene ◽

Rrna Gene Sequence ◽

Phylogenetic Comparison ◽

Gene Sequence Analysis ◽

Culture Independent

ABSTRACT A phylogenetic comparison of microbial communities in hypersaline evaporites was conducted on crusts from Guerrero Negro, Mexico, and Lindsey Lake, New Mexico, using culture-independent rRNA gene sequence analysis. Many sequences were shared between evaporites, which suggests that similar environments select for specific microbial lineages from a global metacommunity.

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