scholarly journals Degradation of nucleic acids with ozone. VI. Labilization of the double-helical structure of calf thymus deoxyribonucleic acid.

1984 ◽  
Vol 32 (9) ◽  
pp. 3636-3640 ◽  
Author(s):  
NARIKO SHINRIKI ◽  
KOZO ISHIZAKI ◽  
SHOKO SATO ◽  
KAZUNOBU MIURA ◽  
KAZUYUKI SAWADAISHI ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Deoxyribonucleic Acid ◽  
Helical Structure ◽  
Double Helical Structure ◽  
Calf Thymus

scholarly journals DNA Interaction and DNA Cleavage Studies of a New Platinum(II) Complex Containing Aliphatic and Aromatic Dinitrogen Ligands

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Nahid Shahabadi ◽  
Soheila Kashanian ◽  
Maryam Mahdavi ◽  
Noorkaram Sourinejad
Keyword(s):  
Dna Cleavage ◽  
Helical Structure ◽  
Dna Interaction ◽  
Van Der Waals Interactions ◽  
Double Helical Structure ◽  
Intercalative Mode ◽  
Physico Chemical ◽  
Calf Thymus ◽  
Enthalpy And Entropy Changes ◽  
Ct Dna

A new Pt(II) complex, [Pt(DIP)(LL)](NO3)2(in which DIP is 4,7-diphenyl-1,10-phenanthroline and LL is the aliphatic dinitrogen ligand,N,N-dimethyl-trimethylenediamine), was synthesized and characterized using different physico-chemical methods. The interaction of this complex with calf thymus DNA (CT-DNA) was investigated by absorption, emission, circular dichroism (CD), and viscosity measurements. The complex binds to CT-DNA in an intercalative mode. The calculated binding constant,Kb, was  M−1. The enthalpy and entropy changes of the reaction between the complex and CT-DNA showed that the van der Waals interactions and hydrogen bonds are the main forces in the interaction with CT-DNA. In addition, CD study showed that phenanthroline ligand insert between the base pair stack of double helical structure of DNA. It is remarkable that this complex has the ability to cleave the supercoiled plasmid.

Introduction to DNA

2018 ◽  
pp. 1-10
Author(s):  
David Bensimon ◽  
Vincent Croquette ◽  
Jean-François Allemand ◽  
Xavier Michalet ◽  
Terence Strick
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Structural Properties ◽  
Helical Structure ◽  
3D Structures ◽  
Double Helical Structure ◽  
Dna And Rna

This chapter provides a quick introduction to the structural properties of nucleic acids (DNA and RNA). It describes the famed double-helical structure of DNA, the more complex 3D structures adopted by RNA, and the random (possibly) twisted coil that nucleic acid can display at large scales.

scholarly journals Zn2+–DNA interactions in aqueous systems: A Raman spectroscopic study

2009 ◽  
Vol 23 (3-4) ◽  
pp. 155-163 ◽  
Author(s):  
Cristina M. Muntean ◽  
Konstantinos Nalpantidis ◽  
Ingo Feldmann ◽  
Volker Deckert
Keyword(s):  
Room Temperature ◽  
Helical Structure ◽  
Intensity Change ◽  
Dna Melting ◽  
Dna Interactions ◽  
Raman Spectroscopic ◽  
Double Helical Structure ◽  
Calf Thymus ◽  
Concentration Changes ◽  
Phosphate Groups

The influence of Zn2+ions on the structure of natural calf thymus DNA was studied by Raman spectroscopy. Measurements were done at room temperature and pH 6.2±0.1, in the presence of 10 mM Na+, and of Zn2+in a concentration range varying between 0 and 250 mM, respectively. No condensation of DNA was observed.As judging from the marker bands near 681 cm−1(dG), 729 cm−1(dA), 752 cm−1(dT), and 787 cm−1(dC, dT) altered nucleoside conformations in these residues are supposed to occur, in different intervals of Zn2+ions concentration. Changes in the conformational marker centered around 835 cm−1, upon Zn2+binding to DNA, were detected. Binding of zinc(II) ions to the charged phosphate groups of DNA, stabilizing the double helical structure, is indicated in the spectra. We have found that binding of metal ions at N3 of cytosine takes place at zinc(II) concentrations between 150–250 mM and interaction of Zn2+ions with adenine is observed in a concentration range from 10 to 250 mM. Binding of zinc(II) ions to N7 of guanine and, possibly, in a lesser extent to adenine was also observed as indicated by the Raman marker bands near 1490 and 1581 cm−1. There is no intensity change of the band at 1668 cm−1, suggesting no change in their base pairing and no change induced in the structure of water by Zn2+cations. No evidence for DNA melting was identified.

scholarly journals The inhibition of deoxyribonucleic acid nucleotidyltransferase by stilboestrol derivatives

1968 ◽  
Vol 108 (5) ◽  
pp. 749-753 ◽  
Author(s):  
A R Fahmy ◽  
K Griffiths
Keyword(s):  
Comparative Study ◽  
Deoxyribonucleic Acid ◽  
Inhibitory Effect ◽  
Effective Inhibitor ◽  
Inhibitory Effects ◽  
Alkyl Substitution ◽  
Competitive Kinetics ◽  
Calf Thymus

The inhibition by diethylstilboestrol of DNA nucleotidyltransferase isolated from calf thymus was studied. The inhibition exercised by diethylstilboestrol appears to obey competitive kinetics with respect to DNA primer. The activities of both replicative and terminal enzymes were affected to the same extent. There was no evidence of binding between DNA and diethylstilboestrol. A comparative study of the inhibitory effects of some stilboestrol derivatives is presented. The alkyl substitution in the αα′-positions seem to alter the inhibitory effect of these compounds: dimethylstilboestrol was more inhibitory than stilbene, and diethylstilboestrol was more inhibitory than dimethylstilboestrol. Hexoestrol, in which the αα′-ethylenic linkage is saturated, was the most effective inhibitor.

Sign in / Sign up

Export Citation Format

Share Document