Effects of Aerobic Training on Gene Expression in Skeletal Muscle of Elderly Men

2005 ◽  
Vol 37 (10) ◽  
pp. 1680-1696 ◽  
Author(s):  
SHLOMIT RADOM-AIZIK ◽  
SHLOMO HAYEK ◽  
IRIS SHAHAR ◽  
GIDEON RECHAVI ◽  
NAFTALI KAMINSKI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Aerobic Training ◽  
Elderly Men

scholarly journals Losartan has no additive effect on the response to heavy-resistance exercise in human elderly skeletal muscle

2018 ◽  
Vol 125 (5) ◽  
pp. 1536-1554 ◽  
Author(s):  
Mette Flindt Heisterberg ◽  
Jesper L. Andersen ◽  
Peter Schjerling ◽  
Alberte Lund ◽  
Simone Dalskov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Angiotensin Ii ◽  
Resistance Training ◽  
Resistance Exercise ◽  
Satellite Cells ◽  
Type I ◽  
Elderly Men ◽  
Fiber Area ◽  
Heavy Resistance Exercise

Our purpose here was to investigate the potential of blocking the angiotensin II type I receptor (AT1R) on the hypertrophy response of elderly human skeletal muscle to 4 mo of heavy-resistance exercise training. Fifty-eight healthy elderly men (+65 yr) were randomized into three groups, consuming either AT1R blocker (losartan, 100 mg/day) or placebo for 4 mo. Two groups performed resistance training (RT) and were treated with either losartan or placebo, and one group did not train but was treated with losartan. Quadriceps muscle biopsies, MR scans, and strength tests were performed at baseline and after 8 and 16 wk. Biopsies were sectioned for immunohistochemistry to determine the number of satellite cells, capillaries, fiber type distribution, and fiber area. Gene expression levels of myostatin, connective tissue, and myogenic signaling pathways were determined by real-time RT-PCR. Four months of heavy-resistance training led in both training groups to expected improvements in quadriceps (∼3–4%) and vastus lateralis (∼5–6%), cross-sectional area, and type II fiber area (∼10–18%), as well as dynamic (∼13%) and isometric (∼19%) quadriceps peak force, but with absolutely no effect of losartan on these outcomes. Furthermore, no changes were seen in satellite cell number with training, and most gene targets failed to show any changes induced by training or losartan treatment. We conclude that there does not appear to be any effect of AT1R blocking in elderly men during 4 mo of resistance training. Therefore, we do not find any support for using AT1R blockers for promoting muscle adaptation to training in humans. NEW & NOTEWORTHY Animal studies have suggested that blocking angiotensin II type I receptor (AT1R) enhances muscle regeneration and prevents disuse atrophy, but studies in humans are limited. Focusing on hypertrophy, satellite cells, and gene expression, we found that AT1R blocking did not result in any greater responses with 4 mo of resistance training. These results do not support previous findings and question the value of blocking AT1R in the context of preserving aging human muscle.

Training Reverses Age-Related Gene Expression Changes In Skeletal Muscle Of Elderly Men

2005 ◽  
Vol 37 (Supplement) ◽  
pp. S243
Author(s):  
Shlomit Radom-Aizik ◽  
Shlomo Hayek ◽  
Gidi Rechavi ◽  
Ninette Amariglio ◽  
Hillel Halkin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Related Gene ◽  
Elderly Men ◽  
Age Related

scholarly journals PL-008 Adaptation of skeletal muscle to aerobic exercise: specific transcriptome response to acute exercise and training

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Daniil Popov ◽  
Pavel Makhnovskii ◽  
Evgeny Lysenko ◽  
Olga Vinogradova
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Circadian Rhythm ◽  
Human Skeletal Muscle ◽  
Acute Exercise ◽  
Aerobic Training ◽  
Contractile Activity ◽  
Training Programme ◽  
Specific Response ◽  
Transcriptome Response

Objective Variety of processes including circadian rhythm and systemic factors affect expression of many genes in skeletal muscle during a day. Therefore, post-exercise gene expression depends on many factors: contractile activity per seas well as circadian rhythm, nerve activity, concentration of different substances in blood, feeding and fasting. In our study, we investigated specific for contractile activity changes in the transcriptome in untrained and trained (after an aerobic training programme) human skeletal muscle. The second goal was to examine effect of aerobic training on gene expression in muscle in basal state. Methods Seven untrained males performed the one-legged knee extension exercise (for 60 min) with the same relative intensity before and after a 2 month aerobic training programme (1 h/day, 5/week). Biopsy samples were taken at rest (basal state, 48 h after the previous exercise), 1 and 4 h after one-legged exercise from m. vastus lateralisof either leg. This approach allowed us to evaluate specific changes in the transcriptome associated with contractile activity. RNA­sequencing (84 samples in total; ~42 million reads/sample) was performed by HiSeq 2500 (Illumina). Results Two months aerobic training increased the aerobic capacity of the knee-extensor muscles (power at anaerobic threshold in incremental one-legged and cycling tests), the maximum rate of ADP-stimulated mitochondrial respiration in permeabilized muscle fibres and amounts of oxidative phosphorylation proteins. After one-legged exercise, expression of many genes was changed in exercised muscle (~1500) as well as in non-exercised muscle (~400). Pronounced changes in gene expression in non-exercised muscle may be associated with many factors, including circadian rhythm (result of GO analysis). To examine transcriptome changes specific for contractile activity, the difference in gene expression between legs was examined. In untrained muscle, one-legged exercise changed expression of ~1200 genes specific for contractile activity at each time point. Despite the same relative intensity of one-legged exercise, transcriptomic response in trained muscle was markedly lower (~300 genes) compare to untrained. We observed a strong overlap between transcriptomic responses (~250 genes) and particularly between enriched transcription factor binding sites in promoters of these genes in untrained and trained muscles. These sets of genes and transcription factors play the key role in adaptation of muscle to contractile activity independently on the level of muscular fitness. Surprisingly, 2 months aerobic training changed the expression of more than 1500 genes in basal state. Noteworthy, these genes demonstrated a small overlap (~200 genes) with genes related to specific response to acute exercise. Moreover, these genes were associated with significantly different biological processes than genes related to specific response to acute exercise. Conclusions Specific for contractile activity changes in the transcriptome in untrained and trained human skeletal muscle were revealed for the first time. After 2 month aerobic training, the specific transcriptome response to acute exercise become much less pronounced. A computational approach reveals common transcription factors important for adaptation of both untrained and trained muscle. We found out that adaptation of muscle to aerobic training associates not only with the transitory changes in gene expression after each exercise, but also with the marked changes in transcriptome in basal state. This work was supported by the Russian Science Foundation (14­15­00768).

scholarly journals Effects of 8 Weeks Aerobic Training on Plasma Ghrelin Level and Ghrelin Lymphocyte Gene Expression in Elderly Men

Salmand ◽  
2019 ◽  
pp. 494-505
Author(s):  
Seyyed Mahdi Ahmadi ◽  
Mehrdad Fathi ◽  
Amir RashidLamir ◽  
Farnaz Aminian
Keyword(s):  
Gene Expression ◽  
Aerobic Training ◽  
Ghrelin Level ◽  
Elderly Men ◽  
Plasma Ghrelin Level

Training Reverses Age-Related Gene Expression Changes In Skeletal Muscle Of Elderly Men

2005 ◽  
Vol 37 (Supplement) ◽  
pp. S243
Author(s):  
Shlomit Radom-Aizik ◽  
Shlomo Hayek ◽  
Gidi Rechavi ◽  
Ninette Amariglio ◽  
Hillel Halkin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Related Gene ◽  
Elderly Men ◽  
Age Related

Gene expression of GLUT4 in skeletal muscle from insulin-resistant patients with obesity, IGT, GDM, and NIDDM

Diabetes ◽  
1992 ◽  
Vol 41 (4) ◽  
pp. 465-475 ◽  
Author(s):  
W. T. Garvey ◽  
L. Maianu ◽  
J. A. Hancock ◽  
A. M. Golichowski ◽  
A. Baron
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Insulin Resistant

PPAR-gamma gene expression is elevated in skeletal muscle of obese and type II diabetic subjects

Diabetes ◽  
1997 ◽  
Vol 46 (7) ◽  
pp. 1230-1234 ◽  
Author(s):  
K. S. Park ◽  
T. P. Ciaraldi ◽  
L. Abrams-Carter ◽  
S. Mudaliar ◽  
S. E. Nikoulina ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Ppar Gamma ◽  
Type Ii

Retinoic Acid Receptor Agonists Suppress Muscle Fatty Infiltration in Mice

2021 ◽  
Vol 49 (2) ◽  
pp. 332-339
Author(s):  
Hideyuki Shirasawa ◽  
Noboru Matsumura ◽  
Masaki Yoda ◽  
Kazumasa Okubo ◽  
Masayuki Shimoda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Rotator Cuff ◽  
Rotator Cuff Tear ◽  
Gene Expression Analysis ◽  
Retinoic Acid Receptor ◽  
Adipogenic Differentiation ◽  
Fatty Infiltration ◽  
Cuff Tear

Background: The infiltration of fat tissue into skeletal muscle, a condition referred to as muscle fatty infiltration or fatty degeneration, is regarded as an irreversible event that significantly compromises the motor function of skeletal muscle. Purpose: To investigate the effect of retinoic acid receptor (RAR) agonists in suppressing the adipogenic differentiation of fibroadipogenic progenitors (FAPs) in vitro and fatty infiltration after rotator cuff tear in mice. Study Design: Controlled laboratory study. Methods: FAPs isolated from mouse skeletal muscle were cultured in adipogenic differentiation medium in the presence or absence of an RAR agonist. At the end of cell culture, adipogenic differentiation was evaluated by gene expression analysis and oil red O staining. A mouse model of fatty infiltration—which includes the resection of the rotator cuff, removal of the humeral head, and denervation the supraspinatus muscle—was used to induce fatty infiltration in the supraspinatus muscle. The mice were orally or intramuscularly administered with an RAR agonist after the surgery. Muscle fatty infiltration was evaluated by histology and gene expression analysis. Results: RAR agonists effectively inhibited the adipogenic differentiation of FAPs in vitro. Oral and intramuscular administration of RAR agonists suppressed the development of muscle fatty infiltration in the mice after rotator cuff tear. In accordance, we found a significant decrease in the number of intramuscular fat cells and suppressed expression in adipogenic markers. RAR agonists also increased the expression of the transcripts for collagens; however, an accumulation of collagenous tissues was not histologically evident in the present model. Conclusion: Muscle fatty infiltration can be alleviated by RAR agonists through suppressing the adipogenic differentiation of FAPs. The results also suggest that RAR agonists are potential therapeutic agents for treating patients who are at risk of developing muscle fatty infiltration. The consequence of the increased expression of collagen transcripts by RAR agonists needs to be clarified. Clinical Relevance: RAR agonists can be used to prevent the development of muscle fatty infiltration after rotator cuff tear. Nevertheless, further studies are mandatory in a large animal model to examine the safety and efficacy of intramuscular injection of RAR agonists.

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