PO-003 Effect of C2C12 Myotubes Function after Electrical Stimulation at Different Time

Objective To study the effect of different time of electrical stimulation on C2C12 myotubes function, and further explore its molecular mechanism. Methods An electrical stimulation was given 7 days after C2C12 myotubes differentiation, of which intensity was 30ms, 3Hz, and the stimulation time was 60mins, 120mins, and 180mins, respectively. A total of four experimental groups, including Con (control group), E60 (60 mins group), E120and E180. Microscope was used to observe the muscular myotubes form; Kits were to detect MDA and ROS; Western blot was used to detect the expression of autophagy proteins and mechanism proteins, including PGC1, p-ULK, SIRT1 and SIRT3. Results Compared with the control group, MDA, ROS, SIRT3 increased significantly in E60 (p<0.05), p-ULK and PGC1 increased significantly (p<0.01), SIRT1 decreased significantly (p<0.05). In E120, MDA、ROS、SIRT3 and PGC1 increased significantly (p<0.01), SOD decreased significantly (p<0.05). In E180, MDA and ROS increased significantly (p<0.01), SOD decreased significantly (p<0.01). Conclusions Moderate electrical stimulation can significantly activate oxidative stress, and further promote SIRT3, PGC1 and p-ULK expression, while excessive stimulation has the opposite effects.

PO-201 Aging attenuates the effect of aerobic capacity in muscle and serum metabolic profile but not in white adipose tissue

Objective Aerobic capacity is a quantitative predictor of the morbidity and mortality in many diverse patient populations. While aging is the main factor affecting aerobic capacity. The present study aimed to assess the effect of aerobic capacity and aging on metabolic profile in rats and to investigate the metabolic interactions between white adipose tissue (WAT), muscle and serum. Methods In this study, we used rat models that were selectively bred to differ in maximal running capacity (High capacity runners (HCR) and Low capacity runners (LCR)). Part of the rats were sacrificed after 9 months and the rest at 21 months. The effect of aerobic capacity on metabolic profile was assessed from 9 months old young rats (HCR-Y and LCR-Y), while the effect of aging on the metabolic profile in different capacity rats was determined comparing 9 months to 21 months old rats (HCR-O and LCR-O). Nuclear magnetic resonance (NMR) spectroscopy was performed to detect the metabolomics of WAT, muscle and serum. Partial least-squares-discriminant analysis (PLS-DA) was used for pattern recognition between HCR-Y and LCR-Y and between HCR-O and LCR-O. Metabolites with variable influence on projection (VIP) >1.0 and p<0.05 were classified as significantly different metabolites between groups. Spearman correlation was used to assess the metabolic interactions between white adipose tissue (WAT), muscle and serum. Results HCR-Y rats had significantly higher skeletal muscle mass-to-body mass ratio (p<0.001), while lower body mass (p<0.001), fat mass (p<0.001), skeletal muscle mass (p=0.035) and fat mass to body mass ratio (p=0.004) than LCR-Y rats. The running capacity of HCR-Y rats was 132.7% (best running speed) better than LCR-Y rats (p<0.001). However, with age, the difference between body compositions between the two capacity groups became insignificant. HCR-O only had significantly lower body mass than the LCR-O (p=0.02). Running capacity (p=0.06) was 86.4% (best running speed) higher in the HCR-O rats than that of the LCR-O rats. PLS-DA revealed marked effects of aerobic capacity on metabolic profile in all three tissue types between HCR-Y and LCR-Y. The metabolic profile classification and prediction was best (i.e. sharper) in muscle than in WAT and serum. In addition, muscle and serum contained more significantly different metabolites than WAT in HCR-Y than in LCR-Y. Pathway analysis of the significantly different metabolites between HCR-Y and LCR-Y revealed that all the pathways belong to the lipid metabolism and amino acid metabolism in muscle while in serum it is only amino acid metabolism. However, in the case of the old groups, the PLS-DA gave reversed results. It revealed that WAT performed best in terms of classification and prediction of metabolites between HCR-O and LCR-O and had the most significantly different metabolites out of the three tissue types. The significantly different metabolites’ pathways belong to lipid metabolism in WAT. When assessing the metabolic interaction between different tissue types, all significantly different metabolites between HCR and LCR rats in young and old groups were moderately or strongly correlated (Spearman correlation between 0.45-0.9) with one or more metabolites in any of the three tissues. Conclusions In this study, we assessed the metabolic profile and body composition of WAT, muscle and serum in young and old rats with different aerobic capacities. We found that aerobic capacity greatly impacts body composition and the metabolic profile in muscle and serum in young rats, however the impact is attenuated with age. In addition, it is aging and not aerobic capacity that had the most influence on WAT metabolites. This suggest that WAT has more important role in aging process than previously assumed.

PO-101 Inhibition of Aerobic Exercise on PKC/CaV1.2 pathway enhanced the function of vascular smooth muscle in hypertension

Objective The purpose of this study was to investigate the effects of aerobic exercise on PKC/CaV1.2 pathway in mesenteric arterial smooth muscle from spontaneously hypertensive rats (SHRs) Methods Twelve-week-old male normotensive Wistar–Kyoto (WKY) rats and SHRs were randomly assigned to sedentary groups (SHR-SED, WKY-SED) and exercise training groups (SHR-EX, WKY-EX). Exercise groups were performed an 8-week moderate-intensity treadmill running. After 8 weeks, vascular contractility of mesenteric arteries was measured. Vascular smooth muscle cells (VSMCs) were obtained with an enzymatic isolation method. CaV1.2 channel currents were examined by using whole-cell patch clamp recording technique. Results 1) Body weight and systolic blood pressure (SBP) in both WKY-EX and SHR-EX were significantly lower than those of their sedentary counterparts (both P＜0.05). Body weight in SHR-SED was remarkably lower than WKY-SED (P＜0.05), while SBP was much higher than WKY-SED (P＜0.05). 2) PDBu (PKC activator) elicited a tension increase, and Gö6976 (PKC inhibitor) induced vasodilation. Both the responses of PDBu and Gö6976 in SHR-SED were notably increased compared with WKY-SED (both P＜0.05), however, exercise training significantly suppressed these increases (both P＜0.05). 3) Nifedipine (CaV1.2 inhibitor) induced vasodilation. Response to nifedipine in SHR-SED was more sensitive than both SHR-EX and WKY-SED (both P＜0.05). 4). The current density of SHR-SED and WKY-EX exhibited an increase compared to the WKY-SED (both P＜0.05), and the current density of the SHR-EX decreased obviously in contrast with SHR-SED (P＜0.05). Besides, PDBu enlarged current density of all the groups, while Gö6976 decreased current density. The increase or decrease amplitude in SHR-SED was significantly higher than WKY-SED (both P＜0.05), whereas exercise training markedly inhibited those responses (both P＜0.05). Conclusions Aerobic exercise efficiently prevents the upregulation of PKC/CaV1.2 pathway in hypertension, and enhances the function of vascular smooth muscle.

PO-236 Associations between sleep quality of college students and different measures of obesity: A cross-sectional study

Objective The majority studies focused on obesity prevention on physical activity and eating behavior. However, epidemiological studies have shown that sleep duration and sleep quality could be an adjustable risk factor for obesity. The aim of this study was to examine the associations of sleep quality with different measurement of obesity in Chinese university students. Methods A total of 481 college students aged 18-25 years volunteered to participate in this study. Sleep quality was assessed by Pittsburgh Sleep Quality Index （PSQI）questionnaire. International Physical Activity Questionnaire （IPAQ）was used to determine the physical activity, Psychological status was assessed by Self-Rating Depression Scale （SDS） and Self-Rating Anxiety Scale (SAS). Body height, weight and waist circumference are measured by a trained researcher. Body composition was evaluated by a bio-impedance device (InBody 230, South Korea). Independent sample t test was applied to compare the sleep characteristics, physical activity, obesity, depression and anxiety in different gender students. The associations among the dependent variables BMI, body fat percentage, and the independent variables age, sleep quality and sleep durations was examined using Multiple linear regression models. SPSS 22.0 (IMB SPSS Inc) was used for all statistical. Results The BMI (22.9±3.4 vs 21.6±3.2, p＜0.001) of male students were significantly higher than that of female, but the percentage of body fat (18.7±6.9 vs 29.7±7.0, p＜0.001) was lower than that of female. We observed a positive association between sleep quality and body fat percentage (β = 0.166, P = 0.037), and a negative association with age (β = -0.166, P = 0.008) in female students. Sleep quality was associated positively with BMI (β = 0.360, P＜0.001), body fat percentage (β = 0.260, P＜0.001), and age (β = 0.215, P＜0.001) in male students; An inverse correlation between sleep duration and BMI (β = -0.141, P = 0.015), body fat percentage (β = -0.134, P = 0.022) was found, and a positive relationship with anxiety scores (β = 0.331, P＜0.001) in male students. while an inverse relationship was found with WHR (β = -0.236, P = 0.001), waist circumference (β = -0.169, P = 0.007), and a positive association between sleep duration with anxiety scores (β = 0.331, P＜0.001) and depression scores (β = 0.415, P＜0.001) in female students. Conclusions The obesity of male and female students goes up with the increase of total score of sleep quality, anxiety and depression, and goes down with the increase of sleep duration, physical activity time and energy consumption. Male obesity increases with age, but female obesity decreases with age. Among the importance of males' sleep duration and sleep quality in the obesity risk assessment, BMI and body fat percentages are more accurate, while for females, BMI and waist circumference is of no statistical significance.

PO-055 Changes of trace elements in skeletal muscle and serum of rats after exercise-induced injury

Objective This study is to investigate the changes of trace elements (Cu, Fe, Zn, Se, Mg) in serum and skeletal muscle of rats after skeletal muscle injury induced by downhill running, and to find out the change regularity of trace elements in the body after exercise injury. To provide experimental basis for how to use trace elements supplements reasonably. Methods Fifty-four healthy male Sprague-Dawley rats aged 8 weeks were randomly divided into two groups: control group (C, N=6) and exercise group (E, N=48, include: 0 h group, 6 h group, 12 h group, 24 h group, 48 h group, 72 h group, 1- week group and 2- week group). The rats in exercise groups run down a 16°incline at 16m/min for 90 minutes. At the end of the exercise, the rats were killed at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h, 1 week and 2 weeks, respectively. The serum was got from the inferior vena cava blood and diluted by 1% nitric acid. The muscle was got from the right side of the rat's sural which were digested by concentrated nitric acid and 30% hydrogen peroxide in 75℃water bath for 20mins. The content of trace elements in muscle and serum were measured by inductively coupled plasma atomic emission spectrometry (ICP-MS). All the data are analyzed and processed by SPSS22.0 statistical software. Results (1) The contents of trace elements in serum showed: Cu, Zn, Mg, Se decreased immediately after exercise, but the Cu still increased to reach a peak at 24h after decreasing, and after 2 weeks the content of Cu was slightly lower than pre-exercise level. However, the content of Zn did not elevate again, it continued declined to the lowest at 24h which was significantly lower than control group (P < 0.05). And after 2 weeks, Zn did not return to the pre-exercise level. The changes of Mg, Se in serum was not statistically significant. There is no difference between 0h and control groups in content of Fe, after that Fe decreased continually and appeared the least value at 24h, the differences between immediate group and control group were statistically significant (P < 0.05). Fe returned to the pre-exercise level after 2 weeks. (2) The contents of trace elements in muscle showed: Most of trace elements increased to the maximum level at 6 h, after that Mg, Fe, Cu decreased to the lowest value at 72 h which were significant lower than 0h group or 6h group (P < 0. 05). ALL the trace elements were lower than pre-exercise level. There was no statistical difference in the content of Se in muscle. Conclusions (1) The different changes of trace elements in skeletal muscle and serum after exercise injury may be due to the redistribution of trace elements caused by the body adaptability. (2) The most obviously changes of trace element in serum and muscle are Cu and Zn. Both of them did not return to the pre-exercise level after 2 weeks, it suggests that the supplement include Cu and Zn may play an important role in recovering after exercise-induced injury.

PO-086 Exercise enhances cardiac antioxidant capacity in diabetic rats through the Keap1/Nrf2 signaling pathway

Objective To investigate the effects of exercise on the myocardial oxidative stress injury of diabetic rats, and discussed the role of Keap1/Nrf2 signaling pathway in this process Methods Tyep 2 diabetic rat model was established by streptozotocin injection through abdominal cavity and high fat diet. The all the diabetic rats were divided into three groups: control group (NC), diabetes group(T2DM) and diabetes exercise group, NC and T2DM group were kept quiet for 8 weeks, T2DME group was trained for 8 weeks. After the exercise, weight, heart weight and blood were measured. MDA, T-SOD and GSH-PX enzyme were measured by biochemical method. Ho-1, Keap1, Nrf2 gene and protein expression were detected by RT-PCR and WesternBlotting. Results Compared with NC group, the weight of rats in the T2DM group significantly decreased [(528+/-71g vs 362+/-33g), P<0.05], HWI significantly increased [(2.845+/-0.22 vs 3.841+/-0.21, P <0.05], blood glucose was significantly increased [(6.4±3.8 vs 26±7.5mmol/L), P <0.01],T-SOD and GSH-PX activity decreased significantly (P<0.05), Ho-1 protein expression increased (P<0.01), Keap1 and Nrf2 showed no significant changes, and Nrf2 nuclear transposition decreased (P<0.05). Compared with the T2DM group, no significant change in body weight and heart weight in the T2DME group, with significant decrease in HWI[（3.841±0.21 vs 3.235±0.23），P＜0.05], with significant decrease in blood glucose [（26.0±7.5 vs 21.0±6.8），P＜0.05]. Ho-1 gene and protein expression increased significantly（P＜0.05and P＜0.01）, with no significant change of Keap1, while Nrf2 expression increased significantly (P < 0.05), and Nrf2 nuclear transposition increased significantly (P < 0.01). Conclusions Exercise activates the myocardial Keap1/Nrf2 signaling pathway in rats, promotes the expression of downstream antioxidant enzymes, increases cardiac antioxidant capacity, and resists diabetic myocardial oxidative stress injury.

PO-288 Research on Norm Construction and Evaluating Levels of 20-m Running in Preschool Children

Objective The study tested young children's 20-m running in Chengdu, analyzed of the characteristics of change in age and gender, to construct the norm and evaluation system of the 20-m running of young children and provide the basis for assessing children's sports ability and physical fitness. The results can used as one of the children's physical fitness evaluation content. Methods The stratified random sampling method was used to select kindergarten children in from 25 kindergartens in a district of Chengdu, and totally, 3089 children of 3-6 years old were tested．The best scores by two trials were used as the 20-m running performance. Results With the growth of the age, young children's 20 m running is gradually increased. There are significant differences in the mean values of boys and girls aged 3, 4, 5 and 6 (F = 228.696, F = 366.477, P < 0.01). Compared with boys and girls of the same age group, boys are superior to girls, and there is no significant difference between boys and girls aged 6 (F = 0.879, P > 0.05). The differences in other groups were statistically significant (F=0.138, F=0.204, F=0.133, P < 0.01). The percentile 10, 25, 50, 75, 90 values of the 20 m running of young children were recorded, respectively, and according to the statistical percentile method to divide the evaluation grade standards, the test results of the 10th, 25th, 75th and 90th percentage site test results were selected to develop the five-level rating system of children's 20 m running. Conclusions With the growth of the age, young children's 20 m running is gradually increased, and there are significant differences between groups. It is suggested that the flexibility of nervous processes, the coordination of the body, the flexibility of joints and muscles, and the strength and endurance of muscles are gradually enhanced in children. Constructed the norm and five-grade evaluation system of preschool children's 20 m running, and provided the basis for formulating the grade standard of preschool children's physique evaluation in the future.

OR-023 Physical evaluation of 6-7 years old female preselected tennis players

Objective Through testing and analysis the characteristics of body shape，body composition，bone growth and physical fitness，hemoglobin, testosterone of 6-7 years old female preselected tennis players，the study aim was to provide reference bases for the early selection of female tennis players. Methods A total of 75 female preselected tennis players(initial selection by the coaches) aged from 6 to 7 years were came from Hebei, Hubei, Qinghai and Inner Mongolia province, who came to Research Center for Heath related Physical Fitness Evaluation of Guangzhou Sport University for physical fitness test from July 2016 to July 2018. The height, weight, length of upper limbs, length of lower limbs, iliac width, shoulder width, body fat, muscle mass, bone age，bone mass density(BMD), anaerobic power and PWC170, reaction time, vertical jump, grip strength, hemoglobin, testosterone were measured using related instruments and methods, and calculated derived indicators BMI, iliac width/shoulder width. Data were compared with the national standard of physical health of students and/or evaluated by deviation method，and correlation had been analysed among physical parameters. Results 1)The 75 female preselected tennis players’ aged from 6 to 7 years height and weight were 128.10±5.32cm and 25.70±3.87kg，and there are 47 girls height upper medium grade level, 60% of which weight was at a moderate level, their BMI were 15.48±1.50kg/m2，and all in the normal range, iliac width/shoulder width ×100 was 76.52±7.00, 70.7% 0f which was above medium grade level, the upper and lower limbs were 54.28±3.60cm and 71.68±5.26cm, girls’ PBF were 21.03±6.44, muscle weight were 18.94±3.00kg, BMD were 2.04±2.20, and no low bone strength were fund; Anaerobic power of all female preselected tennis players were 135.93±31.65kg.cm, and the values of the PWC170 relative weight were 10.79±2.56kg.m/min.kg, reaction time were 0.628±0.128s, vertical jump were 21.13±4.95cm, the grip of right and left hand were 10.36±2.15kg and 10.06±2.40kg, the physical fitness parameters above in the upper middle class were more girls than the lower middle class; The hemoglobin content was 132.15±8.70g/L, which was above the normal level (110 -160g/L), the serum testosterone concentration was 1.52±1.20umol/L, which was much higher than that of normal girls (0-0.7umol/L). 2) When age was controlled, there was negative correlation between T and PFB, vertical jump and body weight, PWC170 and reaction time(P＜0.05), and there was positive correlation between hemoglobin and muscle weight(P＜0.05), height, and vertical jump(P＜0.01), muscle weight and anaerobic power(P<0.01)，anaerobic power and height，weight，BMI，upper and lower limbs(P<0.01, P<0.01, P<0.01, P<0.05, P<0.01)，PWC170 and vertical jump，the grip of right and left hand(P<0.01, P<0.05, P<0.05), vertical jump and upper, lower limbs, iliac width/shoulder width(P<0.01, P<0.01, P<0.01). Conclusions 75 female aged from 6 to 7 years old preselected tennis players’ body shape, physical fitness, physiological and biochemical function were superior to peers, and in those parameters，there were more people in upper middle grade than lower middle grade. There is a certain correlation between body composition, shape and fitness of female preselected tennis players’ aged from 6 to 7 years.

OR-027 Research about Training Monitoring during Different training periods in Chinese Elite Swimmers

Objective Training monitoring has become an integral component of total athlete training. Systematically monitoring the physiological and biochemical variables related to performance helps coaches and athletes to measure the effectiveness of their training programs and decide how to revise or update those programs, especially in swimming training. The key purpose of this study is to evaluate the physical function characteristics during preparation season and stress response during competition training sessions in 2017, and provides the helpful data for scientific training for the implementation of the preparation process. Methods During the preparation period, the National Swimming Team athletes were planed to screen and test the physical function characteristics. There are 39 male athletes and 37 female athletes to participate in the study. Body composition was assessed with dual energy X-ray (DXA). Anthropometric characteristics were assessed using Anthroscan 3D VITUS body scanner, and pulmonary function test using CHEST portable lung function meter(HI-101). During the competition period, the training load monitoring targets were 2 elite players who participated in XVII World Aquatics Championship in Budapest-2017 and the National Games 2017. The monitoring methods mainly included: blood tests (including Hb, CK, BU, testosterone, cortisol and ferritin etc.) were used to monitor the athlete's fitness functional status, and the Z-score method was used to express the index changes of two athletes; blood lactate was used to monitor the training load of athletes, and urine indexes were used to monitor body fluid balance and fatigue. Results 1. During the preparation period, the weight of male athletes is 78.4±8.2kg, the percentage of body fat is 15.9±2.8%, the weight of female athletes is 64.8±6.6kg, and the percentage of body fat is 24.2±3.5%. The vital capacity(VC) was 6.65±0.87 L for males and 4.86±0.69 L for females, the value of forced vital capacity(FVC) was 4.29±1.33 L for males and 3.43±0.96 L for females, and the mean value of ventilation per minute was 148.1±23.12 L for males and 110.4 ± 19.67 L for females. 2. During the competition preparation period, Z score was used to express the blood indicators of two athletes, before the XVII World Aquatics Championship in Budapest-2017, the Z score of Hb, T, T/C ratio and ferritin were (-0.5, 0, -0.4, 1.1) and (-0.8, -0.1, -1.0, 0), respectively. Before the competition of the National Games, the Z scores were (1.0, 0.3, 0.7, 0.6) and (1.4, 1.0, 0.1, -0.6) respectively. 3. Training load monitoring was carried out using the blood lactate control test, as the training load increased, the athletes' performance improved and the lactate level increased slightly. 4. The urine indicator test is used to observe the athlete's dehydration and recovery. On the second morning after the intensive training day, both athletes were negative for urine protein and with normal urine specific gravity. Conclusions 1. The screen and tests about the physical function characteristics of swimming athletes during preparation period is useful to develop a personalized training plan; 2. Z-score is easy and feasible for the elite swimmers to monitoring physical fitness capabilities, and higher Z-score is related with better athletic performance; 3. Blood lactate control test can be used for the training intensity monitoring of swimmers, athletes show higher levels of lactic acid metabolism and higher athletic performance before the competition.

PO-052 Study on the Expression of Inflammatory Factor, chemotactic factor in the Repair of Skeletal Muscle Contusion in Mic

Objective To investigate the regulation of muscle inflammatory factors and chemotactic factors during the repair of skeletal muscle contusion in mice. Methods Forty C57 male mice were needed. Eight for control group (C, n=8) and thirty-tow for muscle contusion group (S, n=32). Subsequently, their gastrocnemius muscles were harvested at 0d, 1d, 3d, 7d, 14d after injury. Hematoxylinand eosin (HE) stain were used to assess the changes of muscle morphology. In addition, the gene expression of inflammatory factors and chemotactic factors was analyzed by real-time polymerase chain reaction. Results 1、Morphology of skeletal muscles showed signs of regeneration at 3d post injury. The maximumamount of regeneration muscle fibers appeared during one week post contusion. Two weeks post-injury morphology of myofibers nearly recovered to normal. 2、After skeletal muscle injury, macrophage markers (CD68, CD163, CD206), a variety of inflammatory factors (IL-1, IL-6, IL-10) were up-regulated. 3、chemotactic factors (CCL2, CCL3, CCL5, CCL8, CXCL9, CXCL10, CXCL12, mRNA) were up-regulated。 Conclusions After skeletal muscle contusion, the expression of a variety of chemotactic factors is up-regulated, which promotes macrophage infiltration and produces a variety of inflammatory factors. They may be involved in the inflammatory response and regeneration process after skeletal muscle contusion.