Blood sampling from the retro-orbital plexus, the saphenous vein and the tail vein in rats: comparative effects on selected behavioural and blood variables

2001 ◽  
Vol 35 (2) ◽  
pp. 131-139 ◽  
Author(s):  
H. van Herck ◽  
V. Baumans ◽  
C. J. W. M. Brandt ◽  
H. A. G. Boere ◽  
A. P. M. Hesp ◽  
...  
Keyword(s):  
Diethyl Ether ◽  
Saphenous Vein ◽  
Blood Sampling ◽  
Halothane Anaesthesia ◽  
Blood Collection ◽  
Sampling Techniques ◽  
Tail Vein ◽  
Ether Anaesthesia ◽  
Urine Production ◽  
Diethyl Ether Anaesthesia

We compared the behaviours of rats, and measured various blood parameters, after three blood sampling techniques: orbital puncture while they were under diethyl-ether anaesthesia, blood collection by tail vein puncture under O2-N 2O-halothane anaesthesia and puncture of the saphenous vein without anaesthesia. Twelve rats were subjected to the three treatments according to a Latin square design. After each treatment, the behaviour of the rats was automatically monitored using the so-called LABORASTM method, which discriminates between grooming, locomotion and inactivity in rats. Based on excitation scores and urine production, it was found that induction of diethyl-ether anaesthesia combined with orbital puncture caused more distress than did the other two blood sampling techniques. The three techniques had no differential effects on the behaviours of grooming, locomotion and inactivity. Collecting 0.5 ml of blood by orbital puncture was ± 7 times faster than doing so by saphenous vein puncture and ± 15 times faster than collecting blood by tail vein puncture while the rats were under O2-N 2O-halothane anaesthesia. The levels of some haematological and plasma variables differed significantly between the three blood collection techniques. These observations may help to select the most appropriate technique of blood sampling with respect to anticipated discomfort in the animals.

Saphenous vein puncture for blood sampling of the mouse, rat, hamster, gerbil, guineapig, ferret and mink

1998 ◽  
Vol 32 (4) ◽  
pp. 364-368 ◽  
Author(s):  
Annelise Hem ◽  
Adrian J. Smith ◽  
Per Solberg
Keyword(s):  
Saphenous Vein ◽  
Blood Sampling ◽  
Blood Collection ◽  
Rapid Sampling

A method is described for blood collection from the lateral saphenous vein. This enables rapid sampling, which if necessary can be repeated from the same site without a need for new puncture wounds. The method is a humane and practical alternative to cardiac and retro-orbital puncture, in species where venepuncture has traditionally been regarded as problematic.

scholarly journals Peripheral vascular changes during diethyl ether anaesthesia

Anaesthesia ◽  
1968 ◽  
Vol 23 (2) ◽  
pp. 203-210 ◽  
Author(s):  
L. McArdle ◽  
G. W. Black ◽  
V. K. N. Unni
Keyword(s):  
Diethyl Ether ◽  
Vascular Changes ◽  
Peripheral Vascular ◽  
Ether Anaesthesia ◽  
Diethyl Ether Anaesthesia

scholarly journals Endocrine stress response in rats subjected to singular orbital puncture while under diethyl-ether anaesthesia

1991 ◽  
Vol 25 (4) ◽  
pp. 325-329 ◽  
Author(s):  
H. Van Herck ◽  
V. Baumans ◽  
S. F. De Boer ◽  
J. Van Der Gugten ◽  
A. B. Van Woerkom ◽  
...  
Keyword(s):  
Stress Response ◽  
Diethyl Ether ◽  
Ether Anaesthesia ◽  
Endocrine Stress Response ◽  
Diethyl Ether Anaesthesia

Comparison of a new device for blood sampling in cats with a vacuum tube collection system—Plasma biochemistry, haematology and practical usage assessment

2007 ◽  
Vol 9 (5) ◽  
pp. 382-386 ◽  
Author(s):  
Brice S Reynolds ◽  
Karine G Boudet ◽  
Mathieu R Faucher ◽  
Claude Germain ◽  
Anne Geffre ◽  
...  
Keyword(s):  
Venous Blood ◽  
Small Diameter ◽  
Plasma Chemistry ◽  
Blood Sampling ◽  
Blood Collection ◽  
Sampling Techniques ◽  
Minimal Risk ◽  
New Device ◽  
Closed Systems ◽  
Serious Injury

Using paediatric devices to collect venous blood from a cephalic vein in cats offers numerous practical advantages over traditional jugular venepuncture and vacuum closed systems: minimal restraint is required; there is minimal risk of serious injury to the cat; the discomfort associated with venepuncture is reduced by the use of small diameter (25 gauge) needles; very small volumes (200 μl) of blood are extracted; and the risk of vein collapse or haematoma is low. The aim of this study was to compare the haematological and plasma chemistry results obtained from six healthy cats using the two sampling techniques. Five plasma biochemical analytes were measured and a complete haematological examination was performed on each specimen. No clinically relevant difference between the two blood sampling techniques was observed for any variable, indicating that paediatric devices provide a useful alternative to vacuum tubes for venous blood collection in the cat.

scholarly journals Histological changes in the orbital region of rats after orbital puncture

1992 ◽  
Vol 26 (1) ◽  
pp. 53-58 ◽  
Author(s):  
H. van Herck ◽  
V. Baumans ◽  
N. R. van der Craats ◽  
A. P. M. Hesp ◽  
G. W. Meijer ◽  
...  
Keyword(s):  
Diethyl Ether ◽  
Random Order ◽  
Harderian Gland ◽  
The Other ◽  
Histological Changes ◽  
Inflammatory Reactions ◽  
Ether Anaesthesia ◽  
Orbital Region ◽  
Diethyl Ether Anaesthesia

To contribute to the assessment of the degree of discomfort in rats after orbital puncture, we have examined the histological changes in the intra-orbital tissues caused by this technique of blood sampling. Orbits were studied from rats euthanized either within 1 min, 4 days, 28 days or 56 days after puncture while under diethyl-ether anaesthesia. The techniques of 2 animal technicians were compared, one using a broken haematocrit capillary and the other using an intact Pasteur's pipette. Non-punctured orbits served as controls. Microscopic slides containing the eye in situ at 2 horizontal levels in the orbital region were examined for 37 parameters; the slides were scored blind and in random order. Orbital puncture caused haemorrhages in the puncture track and, depending on the technique used, also in the periosteum. Four days after puncture, inflammatory reactions were present in the puncture track. Depending on the technique of puncture, these reactions were also seen in the eye muscles and periosteum or in the Harderian gland. Within 4 weeks after puncture, the lesions had healed without detectable scars. The different histological effects of the 2 techniques of orbital puncture are discussed in the light of the characteristics of these techniques.

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