scholarly journals Histological changes in the orbital region of rats after orbital puncture

1992 ◽  
Vol 26 (1) ◽  
pp. 53-58 ◽  
Author(s):  
H. van Herck ◽  
V. Baumans ◽  
N. R. van der Craats ◽  
A. P. M. Hesp ◽  
G. W. Meijer ◽  
...  
Keyword(s):  
Diethyl Ether ◽  
Random Order ◽  
Harderian Gland ◽  
The Other ◽  
Histological Changes ◽  
Inflammatory Reactions ◽  
Ether Anaesthesia ◽  
Orbital Region ◽  
Diethyl Ether Anaesthesia

To contribute to the assessment of the degree of discomfort in rats after orbital puncture, we have examined the histological changes in the intra-orbital tissues caused by this technique of blood sampling. Orbits were studied from rats euthanized either within 1 min, 4 days, 28 days or 56 days after puncture while under diethyl-ether anaesthesia. The techniques of 2 animal technicians were compared, one using a broken haematocrit capillary and the other using an intact Pasteur's pipette. Non-punctured orbits served as controls. Microscopic slides containing the eye in situ at 2 horizontal levels in the orbital region were examined for 37 parameters; the slides were scored blind and in random order. Orbital puncture caused haemorrhages in the puncture track and, depending on the technique used, also in the periosteum. Four days after puncture, inflammatory reactions were present in the puncture track. Depending on the technique of puncture, these reactions were also seen in the eye muscles and periosteum or in the Harderian gland. Within 4 weeks after puncture, the lesions had healed without detectable scars. The different histological effects of the 2 techniques of orbital puncture are discussed in the light of the characteristics of these techniques.

Physiopathogenesis of subdural hematomas

1976 ◽  
Vol 45 (4) ◽  
pp. 393-397 ◽  
Author(s):  
David Glover ◽  
Enrique L. Labadie
Keyword(s):  
Subcutaneous Injection ◽  
The Other ◽  
Content Type ◽  
Inflammatory Reactions ◽  
Subdural Hematomas ◽  
Blood Clots ◽  
Initial Decrease ◽  
Fine Print ◽  
Made In

✓ A previously described experimental hematoma model, achieved by the subcutaneous injection of 12 ml of autologous hemolyzed blood clotted in situ, was made in 33 rats. Seventeen animals served as controls; the other 16 received daily intramuscular injections of dexamethasone. After an initial decrease in size, 47% of the lesions in the control animals enlarged to a mean weight of 12.1 ± 2.5 gm, while the lesions in the 16 steroid-treated rats weighed 3.2 ± 0.70 gm (p < 0.01). Histologically, lesions from the steroid group showed absence of neomembrane formation. These data offer further support to the theory that the neomembrane development and subsequent enlargement of subdural hematomas is due to inflammatory reactions of tissues in contact with large blood clots.

scholarly journals Peripheral vascular changes during diethyl ether anaesthesia

Anaesthesia ◽  
1968 ◽  
Vol 23 (2) ◽  
pp. 203-210 ◽  
Author(s):  
L. McArdle ◽  
G. W. Black ◽  
V. K. N. Unni
Keyword(s):  
Diethyl Ether ◽  
Vascular Changes ◽  
Peripheral Vascular ◽  
Ether Anaesthesia ◽  
Diethyl Ether Anaesthesia

scholarly journals Endocrine stress response in rats subjected to singular orbital puncture while under diethyl-ether anaesthesia

1991 ◽  
Vol 25 (4) ◽  
pp. 325-329 ◽  
Author(s):  
H. Van Herck ◽  
V. Baumans ◽  
S. F. De Boer ◽  
J. Van Der Gugten ◽  
A. B. Van Woerkom ◽  
...  
Keyword(s):  
Stress Response ◽  
Diethyl Ether ◽  
Ether Anaesthesia ◽  
Endocrine Stress Response ◽  
Diethyl Ether Anaesthesia

Blood sampling from the retro-orbital plexus, the saphenous vein and the tail vein in rats: comparative effects on selected behavioural and blood variables

2001 ◽  
Vol 35 (2) ◽  
pp. 131-139 ◽  
Author(s):  
H. van Herck ◽  
V. Baumans ◽  
C. J. W. M. Brandt ◽  
H. A. G. Boere ◽  
A. P. M. Hesp ◽  
...  
Keyword(s):  
Diethyl Ether ◽  
Saphenous Vein ◽  
Blood Sampling ◽  
Halothane Anaesthesia ◽  
Blood Collection ◽  
Sampling Techniques ◽  
Tail Vein ◽  
Ether Anaesthesia ◽  
Urine Production ◽  
Diethyl Ether Anaesthesia

We compared the behaviours of rats, and measured various blood parameters, after three blood sampling techniques: orbital puncture while they were under diethyl-ether anaesthesia, blood collection by tail vein puncture under O2-N 2O-halothane anaesthesia and puncture of the saphenous vein without anaesthesia. Twelve rats were subjected to the three treatments according to a Latin square design. After each treatment, the behaviour of the rats was automatically monitored using the so-called LABORASTM method, which discriminates between grooming, locomotion and inactivity in rats. Based on excitation scores and urine production, it was found that induction of diethyl-ether anaesthesia combined with orbital puncture caused more distress than did the other two blood sampling techniques. The three techniques had no differential effects on the behaviours of grooming, locomotion and inactivity. Collecting 0.5 ml of blood by orbital puncture was ± 7 times faster than doing so by saphenous vein puncture and ± 15 times faster than collecting blood by tail vein puncture while the rats were under O2-N 2O-halothane anaesthesia. The levels of some haematological and plasma variables differed significantly between the three blood collection techniques. These observations may help to select the most appropriate technique of blood sampling with respect to anticipated discomfort in the animals.

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