scholarly journals Effect of base modifications on structure, thermodynamic stability, and gene silencing activity of short interfering RNA

RNA ◽  
2007 ◽  
Vol 13 (8) ◽  
pp. 1301-1316 ◽  
Author(s):  
K. Sipa ◽  
E. Sochacka ◽  
J. Kazmierczak-Baranska ◽  
M. Maszewska ◽  
M. Janicka ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Thermodynamic Stability ◽  
Short Interfering Rna ◽  
Base Modifications ◽  
Interfering Rna

scholarly journals Short interfering RNA-induced gene silencing is transmitted between cells from the mammalian central nervous system

2006 ◽  
Vol 98 (5) ◽  
pp. 1541-1550 ◽  
Author(s):  
Tian-Yong Zhao ◽  
Shi-Ping Zou ◽  
Yelena V. Alimova ◽  
Guoying Wang ◽  
Kurt F. Hauser ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Gene Silencing ◽  
Short Interfering Rna ◽  
Mammalian Central Nervous System ◽  
Interfering Rna

scholarly journals Suppression of short interfering RNA-mediated gene silencing by the structural proteins of hepatitis C virus

2008 ◽  
Vol 89 (11) ◽  
pp. 2761-2766 ◽  
Author(s):  
Jingmin Ji ◽  
Andrea Glaser ◽  
Marion Wernli ◽  
Jan Martin Berke ◽  
Darius Moradpour ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Gene Silencing ◽  
Fluorescent Protein ◽  
Structural Proteins ◽  
Short Interfering Rna ◽  
Argonaute 2 ◽  
Co Precipitation ◽  
Green Fluorescent ◽  
Interfering Rna

Viruses have evolved strategies to overcome the antiviral effects of the host at different levels. Besides specific defence mechanisms, the host responds to viral infection via the interferon pathway and also by RNA interference (RNAi). However, several viruses have been identified that suppress RNAi. We addressed the question of whether hepatitis C virus (HCV) suppresses RNAi, using cell lines constitutively expressing green fluorescent protein (GFP) and inducibly expressing HCV proteins. It was found that short interfering RNA-mediated GFP gene silencing was inhibited when the entire HCV polyprotein was expressed. Further studies showed that HCV structural proteins, and in particular envelope protein 2 (E2), were responsible for this inhibition. Co-precipitation assays demonstrated that E2 bound to Argonaute-2 (Ago-2), a member of the RNA-induced silencing complex, RISC. Thus, HCV E2 that interacts with Ago-2 is able to suppress RNAi.

scholarly journals Gene silencing of human RAMP2 mediated by short-interfering RNA

2006 ◽  
Author(s):  
Giovanna Albertin ◽  
Maristella Ruggero ◽  
Diego Guidolin ◽  
Gastone Nussdorfer
Keyword(s):  
Gene Silencing ◽  
Short Interfering Rna ◽  
Interfering Rna

scholarly journals Short Interfering RNA Accumulation Correlates with Host Recovery in DNA Virus-Infected Hosts, and Gene Silencing Targets Specific Viral Sequences

2006 ◽  
Vol 80 (2) ◽  
pp. 1064-1064 ◽  
Author(s):  
Padmanabhan Chellappan ◽  
Ramachandran Vanitharani ◽  
Justin Pita ◽  
Claude M. Fauquet
Keyword(s):  
Gene Silencing ◽  
Short Interfering Rna ◽  
Dna Virus ◽  
Rna Accumulation ◽  
Interfering Rna ◽  
Viral Sequences
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