scholarly journals p53 Phosphorylation in Mouse Skin and In vitro Human Skin Model by High-dose-radiation Exposure

2005 ◽  
Vol 46 (4) ◽  
pp. 461-468 ◽  
Author(s):  
Manabu KOIKE ◽  
Jun SUGASAWA ◽  
Aki KOIKE ◽  
Yoko KOHNO
Keyword(s):  
Radiation Exposure ◽  
Human Skin ◽  
Mouse Skin ◽  
High Dose ◽  
Skin Model ◽  
Dose Radiation

Evaluation of Cutaneous Damage and Repair Following Acute Solar Ultraviolet Radiation Exposure: Experiments with an In Vitro Reconstructed Human Skin Model and Excised Human Skin

2015 ◽  
Vol 1 (2) ◽  
pp. 109-117 ◽  
Author(s):  
Alexander J. Armento ◽  
Jonathan Oldach ◽  
Gina Stolper ◽  
Maxwell Li ◽  
Michael A. Bachelor ◽  
...  
Keyword(s):  
Ultraviolet Radiation ◽  
Radiation Exposure ◽  
Human Skin ◽  
Skin Model ◽  
Solar Ultraviolet Radiation ◽  
Ultraviolet Radiation Exposure ◽  
Reconstructed Human Skin ◽  
Solar Ultraviolet ◽  
Cutaneous Damage

scholarly journals Development and Evaluation of a Topical Anti-Inflammatory Preparation Containing Dodonaea polyandra Extract

2015 ◽  
Vol 18 (4) ◽  
pp. 578 ◽  
Author(s):  
Bradley S Simpson ◽  
Xianling Luo ◽  
Jiping Wang ◽  
Yunmei Song ◽  
David Claudie ◽  
...  
Keyword(s):  
Phase Separation ◽  
Human Skin ◽  
Dosage Form ◽  
Interleukin 1 ◽  
In Vitro Release ◽  
Skin Inflammation ◽  
Skin Model ◽  
Inflammation Model ◽  
Anti Inflammatory

Purpose: We have previously reported that the Australian Northern Kaanju (Kuuku I’yu) medicinal plant Dodonaea polyandra has anti-inflammatory activity. This is attributed largely to the presence of clerodane diterpenoids contained within the leaf resin. We envisaged developing a topical preparation to treat indications relating to skin inflammation. However, it was unknown whether the resin could be incorporated into a suitable dosage form while retaining the therapeutic value demonstrated in previous work. Therefore, the following study was undertaken to assess parameters of safety and efficacy for a prototype formulation containing the leaf resin extracted from D. polyandra. Methods: Using the assessment criteria of optimum appearance, tactile feeling, spreadability and odour, 78 different formulations were developed. Formulation stability was assessed using a centrifugal test with preparations displaying phase separation further modified or re-formulated. A prototype formulation containing 5% w/w plant resin was selected and subjected to in vitro release studies. This was quantified through HPLC analysis using two major bioactive diterpenoids as reference. The prototype formulation was tested for efficacy in a TPA-induced acute murine skin inflammation model as well as a 3D human skin model for irritancy/toxicity (Epiderm™). Results: The prototype resin cream was a chartreuse-coloured homogenous semisolid preparation that was readily spreadable upon contact with skin with no sensation of tackiness, residual greasiness, or irritation. The optimized cream showed no phase separation after 30 min centrifugation at 825 g. In the TPA-induced inflammation model, the resin formulation significantly reduced ear thickness and interleukin-1 beta levels in mouse ear tissue. The 5% w/w resin cream formulation showed no irritancy in a 3D human skin model. Conclusions: Our results demonstrate that bioactive resin from D. polyandra can be formulated into a stable and non-irritant semi-solid dosage form and reduce parameters of acute skin inflammation in vivo. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Percutaneous absorption and distribution of organophosphates (chlorpyrifos and dichlorvos) following dermal exposure and decontamination scenarios using in vitro human skin model

2014 ◽  
Vol 229 (1) ◽  
pp. 66-72 ◽  
Author(s):  
C.A. Moore ◽  
S.C. Wilkinson ◽  
P.G. Blain ◽  
M. Dunn ◽  
G.A. Aust ◽  
...  
Keyword(s):  
Human Skin ◽  
Percutaneous Absorption ◽  
Dermal Exposure ◽  
Skin Model

An In Vitro Human Skin Model for Assaying Topical Drugs against Dermatophytic Fungi

Skin Models ◽  
1986 ◽  
pp. 257-263 ◽  
Author(s):  
A. M. Kligman ◽  
K. J. McGinley ◽  
A. Foglia
Keyword(s):  
Human Skin ◽  
Skin Model ◽  
Topical Drugs ◽  
Dermatophytic Fungi

Cosmetic efficacy claims in vitro using a three-dimensional human skin model

2001 ◽  
Vol 23 (5) ◽  
pp. 309-318 ◽  
Author(s):  
K. Schlotmann ◽  
M. Kaeten ◽  
A. F. Black ◽  
O. Damour ◽  
M. Waldmann-Laue ◽  
...  
Keyword(s):  
Human Skin ◽  
Three Dimensional ◽  
Skin Model

scholarly journals Effect of Skin Model on In Vitro Performance of an Adhesive Dermally Applied Microarray Coated with Zolmitriptan

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Mahmoud Ameri ◽  
Hayley Lewis ◽  
Paul Lehman
Keyword(s):  
Human Skin ◽  
Ex Vivo ◽  
Full Thickness ◽  
Artificial Membrane ◽  
Skin Model ◽  
Hydrophobic Membrane ◽  
Thickness Skin ◽  
In Vitro Performance

Franz cell studies, utilizing different human skin and an artificial membrane, evaluating the influence of skin model on permeation of zolmitriptan coated on an array of titanium microprojections, were evaluated. Full thickness and dermatomed ex vivo human skin, as well as a synthetic hydrophobic membrane (Strat-M®), were assessed. It was found that the choice of model demonstrated different absorption kinetics for the permeation of zolmitriptan. For the synthetic membrane only 11% of the zolmitriptan coated dose permeated into the receptor media, whilst for the dermatomed skin 85% permeated into the receptor. The permeation of zolmitriptan through full thickness skin had a significantly different absorption profile and time to maximum flux in comparison to the dermatomed skin and synthetic model. On the basis of these results dermatomed skin may be a better estimate of in vivo performance of drug-coated metallic microprojections.

In vitro skin corrosion: Human skin model test – A validation study

2012 ◽  
Vol 26 (6) ◽  
pp. 1072-1074 ◽  
Author(s):  
Gajanan Rajpal Deshmukh ◽  
Kuntrapakam Hema Kumar ◽  
Poojari Venkata Suresh Reddy ◽  
Boddapati Srinivasa Rao
Keyword(s):  
Human Skin ◽  
Model Test ◽  
Validation Study ◽  
Skin Model

Development of genotoxicity test procedures with Episkin®, a reconstructed human skin model: Towards new tools for in vitro risk assessment of dermally applied compounds?

2006 ◽  
Vol 606 (1-2) ◽  
pp. 39-51 ◽  
Author(s):  
Nicole Flamand ◽  
Laurent Marrot ◽  
Jean-Philippe Belaidi ◽  
Linda Bourouf ◽  
Emilie Dourille ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Human Skin ◽  
Skin Model ◽  
Test Procedures ◽  
Genotoxicity Test ◽  
Reconstructed Human Skin
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