scholarly journals The Effect of Different Levels of Humic Acid Fertilizer on Components of Biological Nitrogen Fixation in Cowpea Cultivars in Ahvaz

2014 ◽  
Vol 5 (2) ◽  
pp. 167-174
Keyword(s):  
Nitrogen Fixation ◽  
Humic Acid ◽  
Biological Nitrogen Fixation ◽  
Cowpea Cultivars ◽  
Biological Nitrogen ◽  
Different Levels

scholarly journals Nitrogen fixating ability of mungbean genotypes under different levels of nitrogen application

2016 ◽  
Vol 41 (1) ◽  
pp. 163-171 ◽  
Author(s):  
MA Razzaque ◽  
MM Haque ◽  
MA Karim ◽  
ARM Solaiman
Keyword(s):  
Nitrogen Fixation ◽  
Seed Yield ◽  
Biological Nitrogen Fixation ◽  
Yield Potential ◽  
Nitrogen Application ◽  
Culture Experiment ◽  
N Application ◽  
Biological Nitrogen ◽  
Different Levels ◽  
Pot Culture

A pot culture experiment was conducted at the Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur during kharif II, 2012 to evaluate the nodulation, biological nitrogen fixation and yield potential of genotypes of mungbean under varying levels of N application. There were 10 mungbean genotypes viz. IPSA 12, GK 27, IPSA 3, IPSA 5, ACC12890055, GK 63, ACC12890053, BU mug 4, BARI Mung 6 and Binamoog 5, each genotype treated with six levels of N (0, 20, 40, 60, 80 and 100 kg N ha-1) . Among the genotypes, the IPSA 12 at 40 kg N ha-1 produced the maximum number of nodules (14.54 plant-1) as well as the highest nitrogen fixation (2.684 mmol C2H4). This resulted in the highest seed yield (14.22 g plant-1). The genotype ACC12890053 recorded the lowest nodulation (6 plant-1), nitrogen fixation (1.134) and seed yield (7.33 g plant-1).Bangladesh J. Agril. Res. 41(1): 163-171, March 2016

scholarly journals BIOLOGICAL NITROGEN FIXATION STABILITY OF COWPEA CULTIVARS WITH TROPICAL SEMI-ARID RHIZOBIAL STRAINS

2021 ◽  
Vol 34 (2) ◽  
pp. 359-369
Author(s):  
AMANDA CORDEIRO DE MELO SOUZA ◽  
THIAGO PONTES LIRA ◽  
ANTONIO FÉLIX DA COSTA ◽  
FELIPE JOSÉ CURY FRACETTO ◽  
GISELLE GOMES MONTEIRO FRACETTO ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
Dry Matter ◽  
Block Design ◽  
Nitrogen Accumulation ◽  
Arid Soils ◽  
Cowpea Cultivars ◽  
Nodule Number ◽  
Biological Nitrogen ◽  
Semi Arid

ABSTRACT Cowpeas (Vigna unguiculata L. Walp) are an economically and socially important legume in northern and north-eastern Brazil and can establish effective symbiosis with nitrogen-fixing bacteria. We evaluated the symbiotic compatibility and efficiency of rhizobial strains from Pernambuco semi-arid soils and determined their symbiotic stability on the IPA-206, BR 17-Gurguéia, and BRS Novaera cultivars, selected for different environments. The experiment was conducted in a greenhouse to evaluate a 3 × 28 factorial arrangement (cultivars selected for different environments × inoculation with the currently recommended strain, uninoculated plants with or without mineral nitrogen, and 25 rhizobial strains from semi-arid soils) in a randomized block design with four replicates. We determined nodule number, shoot and root dry matter, nodule dry matter by nodule number, nitrogen accumulated in the shoot by nodule dry matter, nitrogen content and accumulation in the shoot, relative efficiency of the recommended strain based on nitrogen accumulation, and shoot dry matter. Overall, the cultivars responded differently to different strains and cultivar biological nitrogen fixation potential. Strains G7.85 and BR 3262 showed potential for biological nitrogen fixation. BR 3262 was confirmed to be adequate for inoculation of different cowpea cultivars.

scholarly journals Functional analysis of multiple nifB genes of Paenibacillus strains in synthesis of Mo-, Fe- and V-nitrogenases

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Qin Li ◽  
Haowei Zhang ◽  
Liqun Zhang ◽  
Sanfeng Chen
Keyword(s):  
Functional Analysis ◽  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Biological Nitrogen Fixation ◽  
Deletion Analysis ◽  
Phylogeny Analysis ◽  
Biological Nitrogen

Abstract Background Biological nitrogen fixation is catalyzed by Mo-, V- and Fe-nitrogenases that are encoded by nif, vnf and anf genes, respectively. NifB is the key protein in synthesis of the cofactors of all nitrogenases. Most diazotrophic Paenibacillus strains have only one nifB gene located in a compact nif gene cluster (nifBHDKENX(orf1)hesAnifV). But some Paenibacillus strains have multiple nifB genes and their functions are not known. Results A total of 138 nifB genes are found in the 116 diazotrophic Paenibacillus strains. Phylogeny analysis shows that these nifB genes fall into 4 classes: nifBI class including the genes (named as nifB1 genes) that are the first gene within the compact nif gene cluster, nifBII class including the genes (named as nifB2 genes) that are adjacent to anf or vnf genes, nifBIII class whose members are designated as nifB3 genes and nifBIV class whose members are named as nifB4 genes are scattered on genomes. Functional analysis by complementation of the ∆nifB mutant of P. polymyxa which has only one nifB gene has shown that both nifB1 and nifB2 are active in synthesis of Mo-nitrogenase, while nifB3 and nifB4 genes are not. Deletion analysis also has revealed that nifB1 of Paenibacillus sabinae T27 is involved in synthesis of Mo-nitrogenase, while nifB3 and nifB4 genes are not. Complementation of the P. polymyxa ∆nifBHDK mutant with the four reconstituted operons: nifB1anfHDGK, nifB2anfHDGK, nifB1vnfHDGK and nifB2vnfHDGK, has shown both that nifB1 and nifB2 were able to support synthesis of Fe- or V-nitrogenases. Transcriptional results obtained in the original Paenibacillus strains are consistent with the complementation results. Conclusions The multiple nifB genes of the diazotrophic Paenibacillus strains are divided into 4 classes. The nifB1 located in a compact nif gene cluster (nifBHDKENX(orf1)hesAnifV) and the nifB2 genes being adjacent to nif or anf or vnf genes are active in synthesis of Mo-, Fe and V-nitrogenases, but nifB3 and nifB4 are not. The reconstituted anf system comprising 8 genes (nifBanfHDGK and nifXhesAnifV) and vnf system comprising 10 genes (nifBvnfHDGKEN and nifXhesAnifV) support synthesis of Fe-nitrogenase and V-nitrogenase in Paenibacillus background, respectively.

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