Molecular Markers Mapped around the High Shoot Regeneration Capacity Gene Rg-2 in Lycopersicon chilense.

AbstractIt is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.

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The effect of carbon source on plant regeneration in tomato

Horticultural Science ◽

10.17221/3759-hortsci ◽

2011 ◽

Vol 32 (No. 1) ◽

pp. 6-8 ◽

Cited By ~ 2

Author(s):

J. Gubiš ◽

Z. Lajchová ◽

L. Klčová

Keyword(s):

Plant Regeneration ◽

Carbon Source ◽

Shoot Regeneration ◽

Regeneration Capacity ◽

Hypocotyl Explants ◽

Cotyledon Explants ◽

Tomato Cultivars ◽

Number Of Shoots

 The effect of different tomato cultivars and different sugar types (sucrose, glucose and maltose) and concentrations (1.0, 2.0 and 3.0%) on shoot regeneration from aseptically grown hypocotyl and cotyledon explants were studied. Among sugar types, sucrose at a concentration of 3.0% induced the highest number of shoots from both types of explants. In hypocotyl explants, cv. Premium showed the best regeneration capacity (0.23 shoots per explant), and in cotyledon explants, cv. Hana produced the maximal number of shoots (0.43 or 0.37 for media with 2.0% or 3.0% sucrose, respectively).  

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Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

Frontiers in Plant Science ◽

10.3389/fpls.2021.797721 ◽

2021 ◽

Vol 12 ◽

Author(s):

Denis Okello ◽

Sungyu Yang ◽

Richard Komakech ◽

Yuseong Chung ◽

Endang Rahmat ◽

...

Keyword(s):

Medicinal Plant ◽

Shoot Regeneration ◽

Callus Induction ◽

Developmental Stages ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Regeneration Capacity ◽

Root Explants ◽

Indirect Regeneration

The medicinal plant, Aspilia africana, has been traditionally used in several African countries to treat many diseases such as tuberculosis, cough, inflammation, malaria, osteoporosis, and diabetes. In this study, we developed a protocol for in vitro propagation of A. africana using indirect shoot organogenesis from leaf and root explants of in vitro-grown seedlings and assessed the tissues at different developmental stages. The highest callus induction (91.9 ± 2.96%) from leaf explants was in the Murashige and Skoog (MS) medium augmented with 1.0 mg/L 6-Benzylaminopurine (BAP) and 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) while from root explants, the highest callus induction (92.6 ± 2.80%) was in the same plant tissue culture medium augmented with 0.5 mg/L BAP and 1.0 mg/L 2,4-D. The best shoot regeneration capacity from leaf-derived calli (i.e., 80.0 ± 6.23% regeneration percentage and 12.0 ± 6.23 shoots per callus) was obtained in medium augmented with 1.0 mg/L BAP and 0.05 mg/L α-Naphthaleneacetic acid (NAA); the best regeneration capacity for root-derived calli (i.e., 86.7 ± 6.24% shoot regeneration percentage and 14.7 ± 1.11 shoots per callus) was obtained in the MS medium augmented with 1.0 mg/L BAP, 0.05 mg/L NAA, and 0.1 mg/L Thidiazuron (TDZ). Regenerated plantlets developed a robust root system in 1/2 MS medium augmented with 0.1 mg/L NAA and had a survival rate of 93.6% at acclimatization. The in vitro regenerated stem tissue was fully differentiated, while the young leaf tissue consisted of largely unorganized and poorly differentiated cells with large intercellular airspaces typical of in vitro leaf tissues. Our study established a protocol for the indirect regeneration of A. africana and offers a basis for its domestication, large-scale multiplication, and germplasm preservation. To the best of our knowledge, this is the first study to develop an indirect regeneration protocol for A. africana and conduct anatomical assessment through the different stages of development from callus to a fully developed plantlet.

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IMPROVED PLANT REGENERATION OF SOLANUM AND LYCOPERSICON GENOTYPES FROM LONG-TERM CALLUS CULTURE.

HortScience ◽

10.21273/hortsci.25.9.1121b ◽

1990 ◽

Vol 25 (9) ◽

pp. 1121b-1121

Author(s):

Chang-Yeon Yu ◽

John Masiunas

Keyword(s):

Plant Regeneration ◽

Shoot Regeneration ◽

Callus Cultures ◽

Solanum Nigrum ◽

Regeneration Capacity ◽

Regeneration Medium ◽

Solanum Ptycanthum ◽

Shoot Differentiation ◽

Solanum Nigrum L

Repeated callus sub-culture reduce the regeneration capacity in many species. Our studies determined the effect of genotype and medium on regeneration of several Solanum and Lycopersicon genotypes from long-term callus cultures. In the first study, 13 genotypes were transferred to regeneration medium, including: Murashige and Skoog plus Gamborg Vitamins (MG); Murashige and Skoog (MS); Gamborg (GM); and white (WM). The greatest shoot regeneration was on the MG medium, containing the highest levels of thiamine. Shoot differentiation was greatest with 0.2 mg/l IAA and 2 mg/l BA. No plants were regenerated on GM or WM medium. In a second study, the effect of thiamine (0 to 200 mg/l) on shoot regeneration of the L. peruvianum genotypes PI199380, PI126945, PI251301, and PI128652, along with Solanum ptycanthum, Solanum nigrum, and L. esculentum `Diego' was evaluated. Shoot regeneration of Solanum ptycanthum, Solanum nigrum, L. peruvianum PI 199380 and PI25301 was best with 20 mg/l of thiamine.

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Florigen Governs Shoot Regeneration

10.21203/rs.3.rs-450479/v1 ◽

2021 ◽

Author(s):

Yaarit Kutsher ◽

Michal Fisler ◽

Adi DORON-FAIGENBOIM ◽

Moshe Reuveni

Keyword(s):

Nitric Oxide ◽

Shoot Regeneration ◽

Ectopic Expression ◽

Flowering Plants ◽

Regeneration Capacity ◽

No Donor ◽

Tobacco Leaves ◽

Age Related ◽

Delayed Flowering

Abstract It is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.

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