<i>Rice tungro spherical virus</i> resistance into photoperiod-insensitive japonica rice by marker-assisted selection

SUMMARYRice tungro disease (RTD), caused by the simultaneous infection of rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV), is one of the major threats to sustainable rice production in South and Southeast Asia. Transgenic resistance against RTBV has been reported previously using an RNA interference (RNAi) construct (ORF IV of RTBV, placed both in sense and anti-sense orientation under CaMV 35S promoter), in the scented rice line Pusa Basmati-1 (PB-1). This construct was transferred to two high-yielding tungro-susceptible indica rice cultivars (IET4094 and IET4786) from the transgenic PB-1 rice line using back cross breeding till the BC2F3 stage. On challenge inoculation, the progenies (BC2F1) showed mild symptoms of tungro, in contrast to severe symptoms displayed by the recurrent parents. Segregation of the transgene indicated near homozygosity of the plants at the BC2F3 stage, implying that the lines can be used as a valuable resistance source for further breeding against RTD.

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Development of elite breeding lines conferring Bph18 gene-derived resistance to brown planthopper (BPH) by marker-assisted selection and genome-wide background analysis in japonica rice (Oryza sativa L.)

Field Crops Research ◽

10.1016/j.fcr.2010.10.004 ◽

2011 ◽

Vol 120 (2) ◽

pp. 215-222 ◽

Cited By ~ 41

Author(s):

Jung-Pil Suh ◽

Sae-Jun Yang ◽

Ji-Ung Jeung ◽

Alvaro Pamplona ◽

Jeong-Ju Kim ◽

...

Keyword(s):

Oryza Sativa ◽

Marker Assisted Selection ◽

Oryza Sativa L ◽

Brown Planthopper ◽

Japonica Rice ◽

Breeding Lines ◽

Genome Wide ◽

Elite Breeding Lines

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Development of Resistant Gene-Pyramided Japonica Rice for Multiple Biotic Stresses Using Molecular Marker-Assisted Selection

Plant Breeding and Biotechnology ◽

10.9787/pbb.2015.3.4.333 ◽

2015 ◽

Vol 3 (4) ◽

pp. 333-345 ◽

Cited By ~ 11

Author(s):

Jung-Pil Suh ◽

Young-Chan Cho ◽

Yong-Jae Won ◽

Eok-Keun Ahn ◽

Man-Kee Baek ◽

...

Keyword(s):

Molecular Marker ◽

Marker Assisted Selection ◽

Japonica Rice ◽

Biotic Stresses ◽

Resistant Gene

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Near Immunity to Rice Tungro Spherical Virus Achieved in Rice by a Replicase-Mediated Resistance Strategy

Phytopathology ◽

10.1094/phyto.1999.89.11.1022 ◽

1999 ◽

Vol 89 (11) ◽

pp. 1022-1027 ◽

Cited By ~ 30

Author(s):

H. Huet ◽

S. Mahendra ◽

J. Wang ◽

E. Sivamani ◽

C. A. Ong ◽

...

Keyword(s):

Antisense Rna ◽

Rice Tungro Bacilliform Virus ◽

Japonica Rice ◽

Rice Tungro Spherical Virus ◽

Rice Plants ◽

Antisense Orientation ◽

Rice Tungro Disease ◽

Sense Orientation ◽

Moderate Resistance ◽

High Level

Rice tungro disease is caused by rice tungro bacilliform virus (RTBV), which is responsible for the symptoms, and rice tungro spherical virus (RTSV), which assists transmission of both viruses by leafhoppers. Transgenic japonica rice plants (Oryza sativa) were produced containing the RTSV replicase (Rep) gene in the sense or antisense orientation. Over 70% of the plants contained one to five copies of the Rep gene, with integration occurring at a single locus in most cases. Plants producing antisense sequences exhibited significant but moderate resistance to RTSV (60%); accumulation of antisense RNA was substantial, indicating that the protection was not of the homology-dependent type. Plants expressing the full-length Rep gene, as well as a truncated Rep gene, in the (+)-sense orientation were 100% resistant to RTSV even when challenged with a high level of inoculum. Accumulation of viral RNA was low, leading us to conclude that RTSV Rep-mediated resistance is not protein-mediated but is of the cosuppression type. Resistance was effective against geographically distinct RTSV isolates. In addition, RTSV-resistant transgenic rice plants were unable to assist transmission of RTBV. Such transgenic plants could be used in an epidemiological approach to combat the spread of the tungro disease.

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