Real-Time PCR Method Using Capturing Oligo-Immobilized PCR Tubes to Determine the Specific Gene for Soybean and Genetically Modified Soybean in Food Matrices

The objective of the study was to determine qualitatively by validated Real Time PCR method the occurrence of genetically modified maize and soybean in commercial food products from the Greek market. 70 independent samples were collected, including products from different categories (i.e. cereal based, biscuits and snacks) which declared either corn or soybean on the labelling. The result of the study indicated that 37.1% of maize and soy products (n=70) displayed in the Greek market have detectable levels of genetically modified maize or soy. These products were identified by specific primers and included common GMΟ detection primers for 35S and NOS terminator. Adequate repeatability and reproducibility was demonstrated for the applied Real Time PCR method, as evaluated by intra- and inter-laboratory tests.

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Collaborative trial validation of a construct-specific real-time PCR method for detection of genetically modified linseed event ‘CDC Triffid’ FP967

European Food Research and Technology ◽

10.1007/s00217-010-1403-7 ◽

2011 ◽

Vol 232 (3) ◽

pp. 557-561 ◽

Cited By ~ 8

Author(s):

Lutz Grohmann ◽

Ulrich Busch ◽

Sven Pecoraro ◽

Norbert Hess ◽

Klaus Pietsch ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Genetically Modified ◽

Collaborative Trial ◽

Pcr Method

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A novel quadruplex real-time PCR method for simultaneous detection of Cry2Aeand two genetically modified cotton events (GHB119 and T304-40)

BMC Biotechnology ◽

10.1186/1472-6750-14-43 ◽

2014 ◽

Vol 14 (1) ◽

Cited By ~ 6

Author(s):

Xiang Li ◽

Xiuxiu Wang ◽

Jielin Yang ◽

Yueming Liu ◽

Yuping He ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Genetically Modified ◽

Simultaneous Detection ◽

Genetically Modified Cotton ◽

Pcr Method

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Development of a Real-time PCR Method Targeting an Unauthorized Genetically Modified Microorganism Producing Alpha-Amylase

Food Analytical Methods ◽

10.1007/s12161-021-02044-x ◽

2021 ◽

Author(s):

Marie-Alice Fraiture ◽

Ugo Marchesi ◽

Daniela Verginelli ◽

Nina Papazova ◽

Nancy H. C. Roosens

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Genetically Modified ◽

Alpha Amylase ◽

Pcr Analysis ◽

Second Line ◽

Fermentation Products ◽

Detection Strategy ◽

Specificity And Sensitivity ◽

Pcr Method

AbstractUsing a recently developed genetically modified microorganisms (GMM) detection strategy, unexpected contaminations of unauthorized GMM in commercialized microbial fermentation products have been reported. A first-line real-time PCR screening analysis was initially performed to determine the presence of key targets frequently found in genetically modified (GM) bacteria. A second-line real-time PCR analysis was subsequently applied to identify specific GMM, including to date a GM Bacillus velezensis producing protease and a GM B. subtilis producing vitamin B2. In this study, an additional real-time PCR method specific to a newly identified GMM producing alpha-amylase was developed to be integrated in such second-line real-time PCR analysis, allowing to strengthen the GMM detection strategy. This method was successfully validated based on the assessment of its specificity and sensitivity performance. In addition, its applicability was confirmed using several food enzyme products commercialized on the market. Finally, via its transfer to an external laboratory, the transferability of the in-house validated method was positively evaluated, allowing its easy implementation in enforcement laboratories.

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