scholarly journals Kinetics of the heat insolubilisation of soybean 11S protein in a phosphate buffer system.

1988 ◽  
Vol 52 (8) ◽  
pp. 2095-2096 ◽  
Author(s):  
Ken WATANABE
Keyword(s):  
Phosphate Buffer ◽  
Buffer System ◽  
Kinetics Of

Kinetics of the Heat Insolubilisation of Soybean 11S Protein in a Phosphate Buffer System

1988 ◽  
Vol 52 (8) ◽  
pp. 2095-2096
Author(s):  
Ken Watanabe
Keyword(s):  
Phosphate Buffer ◽  
Buffer System ◽  
Kinetics Of

Viral inactivation by potassium ferrate

1995 ◽  
Vol 31 (5-6) ◽  
pp. 165-168 ◽  
Author(s):  
Futaba Kazama
Keyword(s):  
Phosphate Buffer ◽  
Potassium Ferrate ◽  
Sodium Thiosulphate ◽  
Viral Inactivation ◽  
Complete Decomposition ◽  
Kinetics Of ◽  
Oxidative Intermediate

The kinetics of inactivation by potassium ferrate were studied using a bacteriophage, F-specific RNA-coliphage Qβ as a viral model. The inactivation appeared to be expressed by Hom's model in phosphate buffer at pH 6, 7, and 8. The rate of inactivation depended on pH; the lower pH, the faster inactivation observed. To consider the mechanism by which ferrate caused inactivation, the efficiency of inactivation was checked after ferrate decomposition in buffer. Effective inactivation following Hom's model was also observed after the complete decomposition of ferrate ion; however, the efficiency of that inactivation disappeared by the addition of sodium thiosulphate, suggesting that rather long-lived oxidative intermediate was generated by the decomposition of ferrate ion. The intermediate might take part in the inactivation.

[99mTc]Tc-Phosphate-buffer system as a potential tracer for bone imaging

2021 ◽  
Author(s):  
Nara Caroline Pereira ◽  
Juliana de Oliveira Silva ◽  
Frederico B. De Sousa ◽  
Sued Eustáquio Mendes Miranda ◽  
Daniel Crístian Ferreira Soares ◽  
...  
Keyword(s):  
Phosphate Buffer ◽  
Buffer System ◽  
Bone Imaging

Effect of phosphate buffer on the kinetics of glycation of proteins

2005 ◽  
Vol 18 (2) ◽  
pp. 183-186 ◽  
Author(s):  
Herminia Gil ◽  
Daniel Salcedo ◽  
Rom�n Romero
Keyword(s):  
Phosphate Buffer ◽  
Kinetics Of

Kinetics of exsheathment of infective ovine and bovine strongylid larvae in vivo and in vitro

Parasitology ◽  
2002 ◽  
Vol 125 (1) ◽  
pp. 65-70 ◽  
Author(s):  
H. HERTZBERG ◽  
U. HUWYLER ◽  
L. KOHLER ◽  
St REHBEIN ◽  
M. WANNER
Keyword(s):  
Haemonchus Contortus ◽  
Buffer System ◽  
Bicarbonate Concentration ◽  
Infective Larvae ◽  
Nylon Mesh ◽  
Mesh Bags ◽  
Sheep Rumen ◽  
Kinetics Of

The aim of the study was to investigate the longitudinal changes of exsheathment of ovine and bovine 3rd-stage strongylid larvae in an artificial rumen (RUSITEC) and to compare the results with in vivo data obtained from rumen-fistulated sheep. Infective larvae were incubated in nylon mesh bags in the sheep rumen or the RUSITEC apparatus for periods of 1, 6 and 12 h, respectively. The 12 h exsheathment rates in the rumen and the RUSITEC apparatus (in parentheses) were as follows: Haemonchus contortus: 100% (100%), Ostertagia circumcincta: 100% (76%), O. leptospicularis: 100% (100%), O. ostertagi: 53% (59%), Trichostrongylus axei: 100% (100%), T. colubriformis: 37% (36%), Cooperia curticei: 94% (76%), C. oncophora: 95% (89%), Nematodirus filicollis: 0% (N.D.), N. spathiger: 11% (15%), N. battus: 7% (5%), Oesophagostomum venulosum: 17% (9%), Chabertia ovina: 7% (2%), Dictyocaulus filaria: 1% (N.D.). Larvae of Nematodirus spp. and T. colubriformis showed a quick rise of the exsheathment rate 2 h after transfer into the abomasum. These results confirm that exsheathment generally occurs in the part of the gastrointestinal tract immediately anterior to the habitat of the adult parasite. The overall similar course of exsheathment in both systems indicates that the essential stimuli for exsheathment were generated and maintained under in vitro conditions of the artificial rumen. In both systems, the bicarbonate concentration and the pH reflected a similar status of the H2CO3/HCO buffer system, which is known to provide the essential stimuli for larval exsheathment of the abomasal species. These results give evidence that the RUSITEC system represents a valid system for studying the kinetics of exsheathment of strongylid nematodes under in vitro conditions. For 7 of the species investigated the obtained results represent the first data on larval exsheathment in vivo and in vitro.

Open-chain nitrogen compounds. Part XV. A kinetic study of the hydrolysis of 1-aryl-3-aryloxymethyl-3-methyltriazenes and related triazenes

1992 ◽  
Vol 70 (8) ◽  
pp. 2224-2233 ◽  
Author(s):  
Keith Vaughan ◽  
Donald L. Hooper ◽  
Marcus P. Merrin
Keyword(s):  
Rate Constant ◽  
Order Rate Constant ◽  
Buffer System ◽  
Open Chain ◽  
Nucleophilic Displacement ◽  
Ether Oxygen ◽  
Rate Of Hydrolysis ◽  
Electron Withdrawing Group ◽  
Ph Range ◽  
Kinetics Of

The kinetics of hydyrolysis of a series of 1-aryl-3-aryloxymethyl-3-methyltriazenes, Ar-N=N-NMe-CH2OAr′, was studied over the pH range 2–7.5. Reactions were followed by the change in UV absorbance spectra of the triazenes. The aryloxymethyltriazenes decompose more slowly at pH 7.5 than the hydroxymethyltriazenes, Ar-N=NMe-CH2OH; the hydrolysis is favoured by the presence of an electron-withdrawing group in Ar′. A mixed isopropanol/buffer system was developed in order to improve solubility of the aryloxymethyl triazenes. Lowering the pH caused an increase in the rate of hydrolysis and under strongly acidic conditions an electron-withdrawing group in Ar′ actually slows down the reaction. A Hammett plot of the pseudo-first-order rate constant, kobs, is curved, indicating that two or more mechanisms operate simultaneously and that the contribution of each mechanism is substituent-dependent. A plot of kobs vs. [buffer] is linear; the slope of the plot affords the rate constant, kb for the buffer-catalyzed reaction for each substituent. A Hammett plot of kb vs. σ is linear with ρ = +0.55, suggesting that the buffer-catalyzed reaction involves nucleophilic displacement of the phenoxy group by the buffer anion. Further analysis afforded the specific acid-catalyzed rate constants, [Formula: see text], for each substituent; this component of the reaction has a negative ρ, consistent with a mechanism involving protonation at the ether oxygen. The postulation that specific acid catalysis is a component of the reaction mechanism was confirmed by the observation of a solvent deuterium isotope effect, 2.28 > kH/kD > 1.60. Only the p-NO2 and p-CN phenyloxymethyltriazenes showed any spontaneous decomposition.

Condition screening and process investigation of aldose transformation in borate-containing acidic phosphate buffer system under microwave irradiation

RSC Advances ◽  
2014 ◽  
Vol 4 (74) ◽  
pp. 39453-39462 ◽  
Author(s):  
Yani Yan ◽  
Qingbin Wu ◽  
Xiao Guo ◽  
Jinhua Lu ◽  
Zhen Hua Li ◽  
...  
Keyword(s):  
Microwave Irradiation ◽  
Phosphate Buffer ◽  
Transformation Process ◽  
Buffer System

The aldose transformation process in borate-containing acidic phosphate buffer system is systematically studied under microwave irradiation.

scholarly journals High-performance capillary electrophoresis of core histones and their acetylated modified derivatives

1992 ◽  
Vol 283 (2) ◽  
pp. 467-471 ◽  
Author(s):  
H Lindner ◽  
W Helliger ◽  
A Dirschlmayer ◽  
M Jaquemar ◽  
B Puschendorf
Keyword(s):  
Capillary Electrophoresis ◽  
Capillary Zone Electrophoresis ◽  
Rat Liver ◽  
Phosphate Buffer ◽  
High Performance ◽  
Buffer System ◽  
Zone Electrophoresis ◽  
Core Histones ◽  
Capillary Zone ◽  
High Performance Capillary Electrophoresis

By using high-performance capillary electrophoresis, we have successfully separated rat liver core histones into several subfractions. Inconvenient interactions of the highly basic proteins with the capillary wall were eliminated by a phosphate buffer system containing 0.03% hydroxyprophylmethylcellulose. Sample amounts of a few nanolitres were analysed within about 20 min. Multiacetylated histones H4 and H3 from induced Friend erythroleukaemic cells prepurified by h.p.l.c. were clearly separated into their non-acetylated and distinct acetylated forms. Our results illustrate that the application of capillary zone electrophoresis on its own or in combination with h.p.l.c. to the analysis of histones provides an important new alternative to traditional gel electrophoreses.

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