Establishment of the Possible Association between the Presence of Helicobacter Pylori in the Saliva and Gastric Biopsy by Using Polymerase Chain Reaction Technique in Association with Oral Manifestation of Peptic Ulcer Disease

The geographical variation in Helicobacter pylori genotypes is an observed phenomenon. Cytotoxin associated genes A (cagA) and E (cagE), and vacuolating cytotoxin (vacA) genotypes of H. pylori are associated with peptic ulcer disease (PUD). This study compared the distribution of these genotypes in Iranian and Afghani isolates and their association with clinical outcomes. H. pylori infected patients, as proven by positive culture, were recruited prospectively. A total of 70 patients, 55 Iranian (26 men and 29 women, mean age 48 +/- 18 years) and 15 Afghani immigrants (13 men and 2 women, mean age 34.8 +/- 11 years) living in Tehran, Iran were enrolled in this study. DNA was extracted from isolated H. pylori and polymerase chain reaction was carried out to determine the cagA and cagE status and vacA alleles. The number of gastric cancer, peptic ulcer and gastritis cases was 11, 23 and 36, respectively. The cagA positive isolates were more common in Iranian (67%) than Afghani isolates (60%). cagE was positive in 53% of Afghani compared to 51% of Iranian isolates. The most common vacA s-region genotype was s1; 80% in Afghani and 67% in Iranian. The slml was a frequently observed genotype in Afghani strains (53%) while s1m2 (47%) was more common in strains isolated from Iranian patients. There is a difference in the H. pylori strains between Iranian and Afghani groups, for instance Iranian isolates were similar to European isolates while Afghani isolates were similar to isolates from India. However, there was no significant association between cagA, cagE and vacA genotypes and clinical outcomes in Iranian and Afghani patients.

Download Full-text

Usefulness of Helicobacter pylori Detection from Feces Specimens of the Patients with Peptic Ulcer by Polymerase Chain Reaction

Kansenshogaku zasshi ◽

10.11150/kansenshogakuzasshi1970.71.1168 ◽

1997 ◽

Vol 71 (11) ◽

pp. 1168-1171 ◽

Cited By ~ 3

Author(s):

Manabu KUROKAWA ◽

Masayuki MINAMIDE ◽

Masafumi NUKINA ◽

Hisao NAKANISHI ◽

Kanji MIKI ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Chain Reaction ◽

Polymerase Chain

Download Full-text

Molecular Cloning and Sequencing of Helicobacter pylori Urease Genes and Detection of H. pylori Using the Polymerase Chain Reaction Technique

Helicobacter pylori, Gastritis and Peptic Ulcer ◽

10.1007/978-3-642-75315-2_11 ◽

1990 ◽

pp. 74-80

Author(s):

C. L. Clayton ◽

M. J. Pallen ◽

H. Kleanthous ◽

B. W. Wren ◽

S. Tabaqchali

Keyword(s):

Polymerase Chain Reaction ◽

Helicobacter Pylori ◽

Molecular Cloning ◽

Polymerase Chain Reaction Technique ◽

Chain Reaction ◽

Cloning And Sequencing ◽

Polymerase Chain ◽

H Pylori

Download Full-text

Molecular Identification of Helicobacter pylori and IceA Genes Frequency from Dental Plaques Isolated from People Using PCR Method

International Journal of Medical Laboratory ◽

10.18502/ijml.v7i3.4137 ◽

2020 ◽

Author(s):

Zahra Salari ◽

Atefeh Ranjkesh ◽

Emad Behboudi

Keyword(s):

Polymerase Chain Reaction ◽

Helicobacter Pylori ◽

Chain Reaction ◽

Ulcer Disease ◽

Two Samples ◽

Pcr Method ◽

Dental Plaques ◽

Polymerase Chain ◽

H Pylori ◽

Development And Evolution

Background and Aims: Helicobacter pylori (H. pylori) is a gram-negative, microaerophilic, spiral-shaped flagellated bacterium that is urease, catalase and oxidase positive. One of its pathogenicity factors is the iceA gene. H. pylori has recently been recognized as a genetic indicator for the development and evolution of duodenal ulcer disease in the East. This study aimed to determine the presence of this bacterium in gingival plaques in non-endocrine patients in Bojnourd city, and the polymerase chain reaction technique examined the percentage of iceA gene. Materials and Methods: A total of 100 samples of dental plaque were taken and transferred to a tube that has been physiologically placed. After DNA extraction, primer design was performed, and then the polymerase chain reaction was performed for the whole sample. Results: Of 100 samples examined in this study, two samples of H. pylori were positive (2%), and the frequency of the iceA gene of two samples was positive (100%). Conclusion: In the Bojnord city, the frequency of iceA gene in people is high, and the frequency of H. pylori in tooth plaques is low. Also, iceA gene can be considered as an indicator for predicting the contamination and risk of H. pylori infection in the region. To confirm the results, more molecular studies are required in other populations.

Download Full-text