Immunocytochemical component of endocrine cells in pancreatic islets of horses.

In the present study, we investigated types of pancreatic endocrine cells and its respective peptides in the Brazilian sparrow species using immunocytochemistry. The use of polyclonal specific antisera for somatostatin, glucagon, avian pancreatic polypeptide (APP), YY polypeptide (PYY) and insulin, revealed a diversified distribution in the pancreas. All these types of immunoreactive cells were observed in the pancreas with different amounts. Insulin- Immunoreactive cells to (B cells) were most numerous, preferably occupying the central place in the pancreatic islets. Somatostatin, PPA, PYY and glucagon immunoreactive cells occurred in a lower frequency in the periphery of pancreatic islets.

Download Full-text

Proportions of Various Endocrine Cells in the Pancreatic Islets of Wood Mice (Apodemus speciosus)

Anatomia Histologia Embryologia ◽

10.1046/j.1439-0264.1999.00148.x ◽

1999 ◽

Vol 28 (1) ◽

pp. 13-16 ◽

Cited By ~ 14

Author(s):

M. Yukawa ◽

T. Takeuchi ◽

T. Watanabe ◽

S. KITAMURA

Keyword(s):

Pancreatic Islets ◽

Endocrine Cells ◽

Apodemus Speciosus ◽

Wood Mice

Download Full-text

Mutations affecting development of zebrafish digestive organs

Development ◽

10.1242/dev.123.1.321 ◽

1996 ◽

Vol 123 (1) ◽

pp. 321-328 ◽

Cited By ~ 2

Author(s):

M. Pack ◽

L. Solnica-Krezel ◽

J. Malicki ◽

S.C. Neuhauss ◽

A.F. Schier ◽

...

Keyword(s):

Pancreatic Islets ◽

Endocrine Cells ◽

Cell Polarization ◽

Immunoreactive Insulin ◽

Intestinal Epithelial ◽

Carboxypeptidase A ◽

Digestive Organs ◽

Epithelial Development ◽

Pancreatic Exocrine ◽

Anterior Intestine

The zebrafish gastrointestinal system matures in a manner akin to higher vertebrates. We describe nine mutations that perturb development of these organs. Normally, by the fourth day postfertilization the digestive organs are formed, the epithelial cells of the intestine are polarized and express digestive enzymes, the hepatocytes secrete bile, and the pancreatic islets and acini generate immunoreactive insulin and carboxypeptidase A, respectively. Seven mutations cause arrest of intestinal epithelial development after formation of the tube but before cell polarization is completed. These perturb different regions of the intestine. Six preferentially affect foregut, and one the hindgut. In one of the foregut mutations the esophagus does not form. Two mutations cause hepatic degeneration. The pancreas is affected in four mutants, all of which also perturb anterior intestine. The pancreatic exocrine cells are selectively affected in these four mutations. Exocrine precursor cells appear, as identified by GATA-5 expression, but do not differentiate and acini do not form. The pancreatic islets are spared, and endocrine cells mature and synthesize insulin. These gastrointestinal mutations may be informative with regard to patterning and crucial lineage decisions during organogenesis, and may be relevant to diabetes, congenital dysmorphogenesis and disorders of cell proliferation.

Download Full-text

Histological and Histochemical Description of the Pancreas in the Adult Lesser Capybara (Hydrochoerus isthmius)

Revista de Medicina Veterinaria ◽

10.19052/mv.vol1.iss43.9 ◽

2021 ◽

Vol 1 (43) ◽

pp. 17-18

Author(s):

Gabriel Álvarez Otero ◽

Cesar Rojano Bolaño ◽

Juan Carrascal Velásquez

Keyword(s):

Pancreatic Islets ◽

Islets Of Langerhans ◽

Endocrine Cells ◽

Neuroendocrine System ◽

Diffuse Neuroendocrine System ◽

Pancreatic Islets Of Langerhans

The aim of this study was to describe histologically and histochemically the pancreas of three adult Hydrochoerus isthmius, attacked by feral dog in Buenavista, Córdoba, Colombia. A complete necropsy was performed and pancreatic fragments were collected and stored in 10% buffered formalin and Bouin liquid. Then, they were dehydrated, diaphanized and embedded in paraffin, stained with Hematoxylin & Eosin, P.A.S., Gomori trichrome, Grimelius and Masson Fontana modified. The H. isthmius pancreas presented a duodenal mesenteric pattern. The exocrine portion was described as a composite tubuloacinar gland. The endocrine portion of the pancreas was constituted by pancreatic islets of Langerhans and a diffuse neuroendocrine system. The histological and histochemical techniques used allowed us to identify the exocrine and endocrine portion of the organ. It is suggested to complement this study with some special techniques for the identification of specific endocrine cells, such as Beta, Alpha, Delta, Epsilon, PP or Gamma.

Download Full-text