scholarly journals Histological and Histochemical Description of the Pancreas in the Adult Lesser Capybara (Hydrochoerus isthmius)

2021 ◽  
Vol 1 (43) ◽  
pp. 17-18
Author(s):  
Gabriel Álvarez Otero ◽  
Cesar Rojano Bolaño ◽  
Juan Carrascal Velásquez

The aim of this study was to describe histologically and histochemically the pancreas of three adult Hydrochoerus isthmius, attacked by feral dog in Buenavista, Córdoba, Colombia. A complete necropsy was performed and pancreatic fragments were collected and stored in 10% buffered formalin and Bouin liquid. Then, they were dehydrated, diaphanized and embedded in paraffin, stained with Hematoxylin & Eosin, P.A.S., Gomori trichrome, Grimelius and Masson Fontana modified. The H. isthmius pancreas presented a duodenal mesenteric pattern. The exocrine portion was described as a composite tubuloacinar gland. The endocrine portion of the pancreas was constituted by pancreatic islets of Langerhans and a diffuse neuroendocrine system. The histological and histochemical techniques used allowed us to identify the exocrine and endocrine portion of the organ. It is suggested to complement this study with some special techniques for the identification of specific endocrine cells, such as Beta, Alpha, Delta, Epsilon, PP or Gamma.

2001 ◽  
Vol 280 (1) ◽  
pp. E92-E102 ◽  
Author(s):  
Brian W. Tobin ◽  
Sandra K. Leeper-Woodford ◽  
Brian B. Hashemi ◽  
Scott M. Smith ◽  
Clarence F. Sams

The present studies were designed to determine effects of a microgravity model system upon lipopolysaccharide (LPS)-stimulated tumor necrosis factor-α (TNF-α) activity and indexes of insulin and fuel homeostasis of pancreatic islets of Langerhans. Islets (1,726 ± 117, 150 islet equivalent units) from Wistar-Furth rats were treated as 1) high aspect ratio vessel (HARV) cell culture, 2) HARV plus LPS, 3) static culture, and 4) static culture plus LPS. TNF-α (L929 cytotoxicity assay) was significantly increased in LPS-induced HARV and static cultures; yet the increase was more pronounced in the static culture group ( P < 0.05). A decrease in insulin concentration was demonstrated in the LPS-stimulated HARV culture ( P< 0.05). We observed a greater glucose concentration and increased disappearance of arginine in islets cultured in HARVs. Although nitrogenous compound analysis indicated a ubiquitous reliance on glutamine in all experimental groups, arginine was converted to ornithine at a twofold greater rate in the islets cultured in the HARV microgravity model system ( P < 0.05). These studies demonstrate alterations in LPS-induced TNF-α production of pancreatic islets of Langerhans, favoring a lesser TNF activity in the HARV. These alterations in fuel homeostasis may be promulgated by gravity-averaged cell culture methods or by three-dimensional cell assembly.


Metabolism ◽  
1982 ◽  
Vol 31 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Jack Noel ◽  
Alexander Rabinovitch ◽  
Les Olson ◽  
George Kyriakides ◽  
Joshua Miller ◽  
...  

1987 ◽  
Vol 44 (1) ◽  
pp. 75-82 ◽  
Author(s):  
GARTH L. WARNOCK ◽  
DEREK W. R. GRAY ◽  
PHILIP MCSHANE ◽  
MAUREEN PETERS ◽  
PETER J. MORRIS

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