scholarly journals Maturation of Theileria sergenti in salivary glands of Haemaphysalis longicornis induced by temperature stimulation.

1990 ◽  
Vol 52 (3) ◽  
pp. 495-501 ◽  
Author(s):  
Nobuhiro SASAKI ◽  
Kiyoshi TAKAHASHI ◽  
Satoshi KAWAMOTO ◽  
Takashi KUROSAWA ◽  
Mitsuo SONODA ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Haemaphysalis Longicornis ◽  
Temperature Stimulation

scholarly journals Development of Babesia ovata in the Salivary Glands of the Nymphai Tick, Haemaphysalis longicornis.

1994 ◽  
Vol 56 (1) ◽  
pp. 207-209 ◽  
Author(s):  
Seiichi HIGUCHI ◽  
Hideki OYA ◽  
Fumio HOSHI ◽  
Seiichi KAWAMURA ◽  
Yoshio YASUDA
Keyword(s):  
Salivary Glands ◽  
Haemaphysalis Longicornis ◽  
Babesia Ovata

scholarly journals A U.S. isolate of Theileria orientalis, Ikeda genotype, is transmitted to cattle by the invasive Asian longhorned tick, Haemaphysalis longicornis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Kelcey D. Dinkel ◽  
David R. Herndon ◽  
Susan M. Noh ◽  
Kevin K. Lahmers ◽  
S. Michelle Todd ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Clinical Signs ◽  
Adult Tick ◽  
Control Measures ◽  
Asia Pacific ◽  
Haemaphysalis Longicornis ◽  
Cattle Industry ◽  
Theileria Orientalis ◽  
And Control ◽  
The U.S

Abstract Background Theileria orientalis is a tick-borne hemoparasite that causes anemia, ill thrift, and death in cattle globally. The Ikeda strain of T.orientalis is more virulent than other strains, leading to severe clinical signs and death of up to 5% of affected animals. Within the Asia–Pacific region, where it affects 25% of Australian cattle, T.orientalis Ikeda has a significant economic impact on the cattle industry. In 2017, T.orientalis Ikeda was detected in a cattle herd in Albermarle County, Virginia, United States. Months earlier, the U.S. was alerted to the invasion of the Asian longhorned tick, Haemaphysalis longicornis, throughout the eastern U.S. Abundant H.longicornis ticks were identified on cattle in the T.orientalis-affected herd in VA, and a subset of ticks from the environment were PCR-positive for T.orientalis Ikeda. A strain of T.orientalis from a previous U.S. outbreak was not transmissible by H.longicornis; however, H.longicornis is the primary tick vector of T.orientalis Ikeda in other regions of the world. Thus, the objective of this study was to determine whether invasive H.longicornis ticks in the U.S. are competent vectors of T.orientalis Ikeda. Methods Nymphal H.longicornis ticks were fed on a splenectomized calf infected with the VA-U.S.-T.orientalis Ikeda strain. After molting, a subset of adult ticks from this cohort were dissected, and salivary glands assayed for T.orientalis Ikeda via qPCR. The remaining adult ticks from the group were allowed to feed on three calves. Calves were subsequently monitored for T.orientalis Ikeda infection via blood smear cytology and PCR. Results After acquisition feeding on a VA-U.S.-T.orientalis Ikeda-infected calf as nymphs, a subset of molted adult tick salivary glands tested positive by qPCR for T.orientalis Ikeda. Adult ticks from the same cohort successfully transmitted T.orientalis Ikeda to 3/3 naïve calves, each of which developed parasitemia reaching 0.4–0.9%. Conclusions Our findings demonstrate that U.S. H.longicornis ticks are competent vectors of the VA-U.S.-T.orientalis Ikeda strain. This data provides important information for the U.S. cattle industry regarding the potential spread of this parasite and the necessity of enhanced surveillance and control measures.

scholarly journals A U.S. Isolate of Theileria Orientalis, Ikeda Genotype, is Transmitted to Cattle by the Invasive Asian Longhorned Tick, Haemaphysalis Longicornis

2020 ◽  
Author(s):  
Kelcey D Dinkel ◽  
David Herndon ◽  
Susan M Noh ◽  
Kevin K Lahmers ◽  
S. Michelle Todd ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Clinical Signs ◽  
Adult Tick ◽  
Control Measures ◽  
Asia Pacific ◽  
Haemaphysalis Longicornis ◽  
Cattle Industry ◽  
Theileria Orientalis ◽  
And Control ◽  
The U.S

Abstract BACKGROUNDTheileria orientalis is a tick-borne hemoparasite that causes anemia, ill thrift, and death in cattle globally. The Ikeda strain of T. orientalis is more virulent than other strains, leading to severe clinical signs and death in less than 5% of affected animals. Within the Asia-Pacific region, where it affects 25% of Australian cattle, T. orientalis Ikeda has a significant economic impact on the cattle industry. In 2017, T. orientalis Ikeda was detected in a cattle herd in Albermarle County, Virginia, United States. Months earlier, the U.S. was alerted to the invasion of the Asian longhorned tick, Haemaphysalis longicornis, throughout the eastern U.S. Abundant H. longicornis ticks were identified on cattle in the T. orientalis-affected herd in VA, and a subset of ticks from the environment were PCR-positive for T. orientalis Ikeda. A strain of T. orientalis from a previous U.S. outbreak was not transmissible by H. longicornis; however, H. longicornis is the primary tick vector of T. orientalis Ikeda in other regions of the world. Thus, the objective of this study was to determine whether invasive H. longicornis ticks in the U.S. are competent vectors of T. orientalis Ikeda. METHODSNymphal H. longicornis ticks were fed on a splenectomized calf infected with the VA-U.S.-T. orientalis Ikeda strain. After molting, a subset of adult ticks from this cohort were dissected, and salivary glands assayed for T. orientalis Ikeda via qPCR. The remaining adult ticks from the group were allowed to feed on three calves. Calves were subsequently monitored for T. orientalis Ikeda infection via blood smear cytology and PCR.RESULTSAfter acquisition feeding on a VA-U.S.-T. orientalis Ikeda-infected calf as nymphs, a subset of molted adult tick salivary glands tested positive by qPCR for T. orientalis Ikeda. Adult ticks from the same cohort successfully transmitted T. orientalis Ikeda to 3/3 naïve calves, each of which developed parasitemia reaching 0.4-0.9%.CONCLUSIONSOur findings demonstrate that U.S. H. longicornis ticks are competent vectors of the VA-U.S.-T. orientalis Ikeda strain. This data provides important information for the U.S. cattle industry regarding the potential spread of this parasite and the necessity of enhanced surveillance and control measures.

scholarly journals Salivary gland proteome analysis of developing adult female Haemaphysalis longicornis ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Shuguang Ren ◽  
Baowen Zhang ◽  
Xiaomin Xue ◽  
Xiaoshuang Wang ◽  
Huaqu Zhao ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Citrate Synthase ◽  
Molecular Motor ◽  
Tca Cycle ◽  
Ixodid Ticks ◽  
Haemaphysalis Longicornis ◽  
Dynamic Changes ◽  
Critical Weight ◽  
Related Proteins

Abstract Background Ticks are notorious blood-feeding arthropods that can spread a variety of deadly diseases. The salivary gland is an important organ for ticks to feed on blood, and this organ begins to develop rapidly when ixodid ticks suck blood. When these ticks reach a critical weight, the salivary glands stop developing and begin to degenerate. The expression levels of a large number of proteins during the development and degeneration of salivary glands change, which regulate the biological functions of the salivary glands. Furthermore, to the best of our knowledge, there are only a few reports on the role of molecular motor and TCA cycle-related proteins in the salivary glands of ticks. Results We used iTRAQ quantitative proteomics to study the dynamic changes in salivary gland proteins in female Haemaphysalis longicornis at four feeding stages: unfed, partially fed, semi-engorged and engorged. Using bioinformatics methods to analyze the dynamic changes of a large number of proteins, we found that molecular motor and TCA cycle-related proteins play an important role in the physiological changes of the salivary glands. The results of RNAi experiments showed that when dynein, kinesin, isocitrate dehydrogenase and citrate synthase were knocked down independently, the weight of the engorged female ticks decreased by 63.5%, 54.9%, 42.6% and 48.6%, respectively, and oviposition amounts decreased by 83.1%, 76.0%, 50.8%, and 55.9%, respectively, and the size of type III acini of females salivary glands decreased by 35.6%, 33.3%, 28.9%, and 20.0%, respectively. Conclusions The results showed that the expression of different types of proteins change in different characteristics in salivary glands during the unfed to engorged process of female ticks. Corresponding expression changes of these proteins at different developmental stages of female ticks are very important to ensure the orderly development of the organ. By analyzing these changes, some proteins, such as molecular motor and TCA cycle-related proteins, were screened and RNAi carried out. When these mRNAs were knocked down, the female ticks cannot develop normally. The research results provide a new protein target for the control of ticks and tick-borne diseases.

scholarly journals A novel defensin-like peptide from salivary glands of the hard tick,Haemaphysalis longicornis

2010 ◽  
pp. NA-NA
Author(s):  
Xiangyun Lu ◽  
Qiaolin Che ◽  
Yi Lv ◽  
Meijuan Wang ◽  
Zekuan Lu ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Hard Tick ◽  
Haemaphysalis Longicornis

scholarly journals Data from expressed sequence tags from the organs and embryos of parthenogenetic Haemaphysalis longicornis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Rika Umemiya-Shirafuji ◽  
Jinlin Zhou ◽  
Min Liao ◽  
Badgar Battsetseg ◽  
Damdinsuren Boldbaatar ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Expressed Sequence Tags ◽  
Tick Species ◽  
Fat Body ◽  
Cdna Libraries ◽  
Haemaphysalis Longicornis ◽  
Data Resource ◽  
Biological Studies ◽  
Wide Range ◽  
Expressed Sequence

Abstract Objectives Haemaphysalis longicornis is the most important tick species in Japan and has a wide range of vector capacity. Due to its veterinary and medical importance, this tick species has been used as a model for tick/vector biological studies. To identify the key molecules associated with physiological processes during blood feeding and embryogenesis, full-length cDNA libraries were constructed using the fat body, hemocytes-containing hemolymph, midgut, ovary and salivary glands of fed females and embryos of the laboratory colony of parthenogenetic H. longicornis. The sequences of cDNA from the salivary glands had been already released. However, the related information is still poor, and the other expressed sequence tags have not yet been deposited. Data description A total of 39,113 expressed sequence tags were obtained and deposited at the DNA DataBank of Japan. There were 7745 sequences from embryos, 7385 from the fat body, 8303 from the hemolymph including hemocytes, 7385 from the midgut, and 8295 from the ovary. The data, including expressed sequence tags from the salivary glands was summarized into Microsoft Excel files. Sharing this data resource with the tick research community will be valuable for the identification of novel genes and advance the progress of tick research.

scholarly journals Identification of Genes Encoding Cement-Like Antigens Expressed in the Salivary Glands of Haemaphysalis longicornis

2006 ◽  
Vol 68 (11) ◽  
pp. 1155-1160 ◽  
Author(s):  
Thasaneeya HARNNOI ◽  
Takeshi SAKAGUCHI ◽  
Xuenan XUAN ◽  
Kozo FUJISAKI
Keyword(s):  
Salivary Glands ◽  
Haemaphysalis Longicornis ◽  
Genes Encoding
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