Typing of Avian Infectious Bronchitis Virus Isolates in Hiroshima Prefecture by RT-PCR and Restriction Enzyme Analysis

1998 ◽  
Vol 51 (12) ◽  
pp. 718-721 ◽  
Author(s):  
Hideyuki YAMASHITA ◽  
Zhifeng LIN
Keyword(s):  
Restriction Enzyme ◽  
Infectious Bronchitis Virus ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Avian Infectious Bronchitis Virus ◽  
Rt Pcr ◽  
Infectious Bronchitis ◽  
Hiroshima Prefecture ◽  
Virus Isolates

Comparative Evaluation of In Vitro and In Vivo Assays for the Detection of Avian Infectious Bronchitis Virus as a Contaminant of Live Poultry Vaccines

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis
Keyword(s):  
Infectious Bronchitis Virus ◽  
Serological Response ◽  
Avian Infectious Bronchitis Virus ◽  
Animal Testing ◽  
Rt Pcr ◽  
Pcr Assay ◽  
In Vivo Test ◽  
Infectious Bronchitis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.

Genetic Diversity of Avian Infectious Bronchitis Virus Isolates in Korea Between 2003 and 2006

2008 ◽  
Vol 52 (2) ◽  
pp. 332-337 ◽  
Author(s):  
Eun-Kyoung Lee ◽  
Woo-Jin Jeon ◽  
Youn-Jeong Lee ◽  
Ok-Mi Jeong ◽  
Jun-Gu Choi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Infectious Bronchitis Virus ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Virus Isolates

scholarly journals Genetic grouping of avian infectious bronchitis virus isolated in Brazil based on RT-PCR/RFLP analysis of the S1 gene

2008 ◽  
Vol 28 (3) ◽  
pp. 190-194 ◽  
Author(s):  
Maria de Fátima S. Montassier ◽  
Liana Brentano ◽  
Hélio J. Montassier ◽  
Leonardo J. Richtzenhain
Keyword(s):  
Infectious Bronchitis Virus ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Avian Infectious Bronchitis Virus ◽  
Rt Pcr ◽  
Infectious Bronchitis ◽  
Glycoprotein Gene ◽  
Pcr Rflp ◽  
Commercial Poultry ◽  
Chicken Eggs

Twelve Brazilian isolates and one reference vaccine strain of avian infectious bronchitis virus (IBV) were propagated in embryonating chicken eggs. The entire S1 glycoprotein gene of these viruses was analysed by reverse-transcriptase-polymerase chain reaction and restriction fragment length polymorphism (RT-PCR-RFLP), using the restriction enzymes HaeIII, XcmI and BstyI. The RFLP patterns led to the classification of these isolates into five distinct genotypes: A, B, C, D and Massachusetts. Five of twelve isolates were grouped in Massachusetts genotype and the remaining seven viruses were classified into four distinct genotypes: A (2), B (2), C (2) or D (1). Such genotyping classification agreed with previous immunological analysis for most of these viruses, highlighting the occurrence of a relevant variability among the IBV strains that are circulating in Brazilian commercial poultry flocks.

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