Antibiofilm activity and cytotoxicity of silk sericin against Streptococcus mutans bacteria in biofilm: an in vitro study

Objective: To investigate the potential of sericin extracted by different methods to inhibit biofilm formation (prevention) and disrupt already formed biofilm (treatment). Method: In this in vitro study, sericin was extracted by heat, acid, alkali and urea. Streptococcus mutans bacteria were cultivated in the presence of various concentrations of sericin to evaluate antibiofilm formation using cell density assay (inhibition effect before biofilm formed). Conversely, various concentrations of sericin were added to a biofilm already formed by Streptococcus mutans bacteria, and the viability of bacteria assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (disruption effects after biofilm formed). Structures of extracted sericin were evaluated using circular dichroism and Fourier-transform infrared spectrometer. Results: The urea-extracted sericin at all concentrations (12.5mg/ml, 25mg/ml, 50mg/ml and 100mg/ml) showed the highest potential antibiofilm activity in terms of both inhibition and disruption effects, compared with sericin extracted by heat, acid or alkali. The heat-extracted and acid-extracted sericin were found to reduce the biofilm formation dose-dependently, while the alkali-extracted sericin did not show either inhibition or disruption effect on the bacterial biofilm. The urea-extracted sericin also killed the bacteria residing within the biofilm, possibly due to its modified structure which may destabilise the bacterial cell wall, leading to membrane disintegration and, finally, cell death. Conclusion: Our results demostrated the antibiofilm activity of sericin. This could form the basis of further research on the mechanism and application of sericin as a novel antibiofilm agent.

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THE EFFECT OF CURCUMA XANTHORRHIZA - ETHANOL EXTRACT TO BIOFILM FORMATION OF STREPTOCOCCUS MUTANS AND AGGREGATIBACTER ACTINOMYCETEMCOMITANS (DENTAL BIOFILM RESEARCH: IN VITRO STUDY)

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2017.v9s1.14_16 ◽

2017 ◽

Vol 9 ◽

pp. 20

Author(s):

Fidhianissa . . ◽

Hedijanti Joenoes ◽

Ariadna A Djais

Keyword(s):

Streptococcus Mutans ◽

Biofilm Formation ◽

In Vitro Study ◽

Ethanol Extract ◽

Single Species ◽

Vitro Study ◽

Bacterial Suspension ◽

Dental Biofilm ◽

Curcuma Xanthorrhiza

Objective: This in vitro study aimed to analyze the mass ratio of single- and dual-species Streptococcus mutans and Aggregatibacteractinomycetemcomitans biofilm after exposure to Curcuma xanthorrhiza ethanol extract (Xan).Methods: A bacterial suspension in brain heart infusion medium, enriched with 0.2% sucrose, was exposed to the Xan, incubated for 18 hrs, andanalyzed using a crystal violet assay.Results: This research concluded that the minimum inhibitory concentration of ethanol-temulawak extract against S. mutans was 5%, while theminimum bactericidal concentration was 15%.Conclusions: Xan prevented biofilm formation of single-species S. mutans and dual-species S. mutans and A. actinomycetemcomitans more effectivelythan it did single-species A. actinomycetemcomitans.

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The effect of photodynamic therapy by gold nanoparticles on Streptococcus mutans and biofilm formation: an in vitro study

Lasers in Medical Science ◽

10.1007/s10103-021-03422-x ◽

2021 ◽

Author(s):

Fatemeh Lavaee ◽

Mohammad Motamedifar ◽

Ghazal Rafiee

Keyword(s):

Gold Nanoparticles ◽

Photodynamic Therapy ◽

Streptococcus Mutans ◽

Biofilm Formation ◽

In Vitro Study ◽

Vitro Study

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Triterpene Derivatives as Relevant Scaffold for New Antibiofilm Drugs

Biomolecules ◽

10.3390/biom9020058 ◽

2019 ◽

Vol 9 (2) ◽

pp. 58 ◽

Cited By ~ 9

Author(s):

Gloria Narjara Santos da Silva ◽

Muriel Primon-Barros ◽

Alexandre José Macedo ◽

Simone Cristina Baggio Gnoatto

Keyword(s):

Staphylococcus Epidermidis ◽

Biofilm Formation ◽

Mammalian Cells ◽

Antibacterial Effect ◽

Bacterial Biofilm ◽

Antibiofilm Activity ◽

Vero Cell Line ◽

Diphenyltetrazolium Bromide ◽

Green Monkey

New medicines for the treatment of bacterial biofilm formation are required. For thisreason, this study shows the in vitro activity of betulinic acid (BA), ursolic acid (UA) and their twentyderivatives against planktonic and biofilm cells (gram-positive bacterial pathogens: Enterococcusfaecalis, Staphylococcus aureus and Staphylococcus epidermidis). We evaluated the antibiofilm activity(through the crystal violet method), as well as the antibacterial activity via absorbance (OD600) atconcentrations of 5, 25 and 100 μM. Likewise, the cytotoxicity of all compounds was evaluated on akidney African green monkey (VERO) cell line at the same concentration, by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) methodology. We verified for the first timewhether different groups at carbon 3 (C-3) of triterpenes may interfere in the antibiofilm activity withminimal or no antibacterial effect. After the screening of 22 compounds at three distinctconcentrations, we found antibiofilm activity for eight distinct derivatives without antibiotic effect.In particular, the derivative 2f, with an isopentanoyl ester at position C-3, was an antibiofilm activityagainst S. aureus without any effect upon mammalian cells.

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