staphylococcus epidermidis
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2024 ◽  
Vol 84 ◽  
Author(s):  
R. Ullah ◽  
A. W. Qureshi ◽  
A. Sajid ◽  
I. Khan ◽  
A. Ullah ◽  
...  

Abstract Fish is the main source of animal protein for human diet. The aim of this study was to find out prevalence of pathogenic bacteria of two selected economically important fish of Pakistan namely Mahseer (Tor putitora) and Silver carp (Hypophthalmichthys molitrix). Live fish samples from hatcheries and dead fish samples from different markets of study area were randomly collected. The fish samples were analyzed for isolation, identification and prevalence of bacteria. The isolated bacteria from study fish were identified through biochemical test and about 10 species of pathogenic bacteria were identified including the pathogenic bacteria to human and fish namely, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus iniae, Serratia spp. Citrobacter spp. Stenotrophomonas spp. Bacillus spp. and Salmonella spp. The bacterial percentage frequency of occurrence in Silver carp and Mahseer fish showed Pseudomonas aeruginosa 21.42%, Staphylococcus epidermidis 17.85%, Escherichia coli 11.90%, Staphylococcus aureus 9.52%, Citrobacter spp. 9.52%, Serratia spp. 8.33%, Streptococcus iniae 7.14%, Stenotrophomonas spp. 5.95%, Bacillus spp. 4.76% and Salmonella spp. 3.57%. The study revealed that Fish samples of Mahseer and Silver carp that were collected from markets have found more isolates (10 bacterial species) than did the fresh fish pond samples (03 bacterial species) of hatcheries. The occurrence of pathogenic bacteria in study fish showed risk factor for public health consumers.


Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 487
Author(s):  
Jolanta Polak ◽  
Marcin Grąz ◽  
Kamila Wlizło ◽  
Katarzyna Szałapata ◽  
Justyna Kapral-Piotrowska ◽  
...  

Fungal laccase obtained from a Cerrena unicolor strain was used as an effective biocatalyst for the transformation of 8-anilino-1-naphthalenesulfonic acid into a green-coloured antibacterial compound, which can be considered as both an antimicrobial agent and a textile dye, simultaneously. The process of biosynthesis was performed in buffered solutions containing methanol as a co-solvent, allowing better solubilisation of substrate. The transformation process was optimised in terms of the buffer pH value, laccase activity, and concentrations of the substrate and co-solvent. The crude product obtained exhibited low cytotoxicity, antibacterial properties against Staphylococcus aureus and Staphylococcus epidermidis, and antioxidant properties. Moreover, the synthesised green-coloured compound proved non-allergenic and demonstrated a high efficiency of dyeing wool fibres.


Author(s):  
Vânia Gaio ◽  
Tânia Lima ◽  
Manuel Vilanova ◽  
Nuno Cerca ◽  
Angela França

Staphylococcus epidermidis biofilm cells are characterized by increased antimicrobial tolerance and improved ability to evade host immune system defenses. These features are, in part, due to the presence of viable but non-culturable (VBNC) cells. A previous study identified genes potentially involved in VBNC cells formation in S. epidermidis biofilms, among which SERP1682/1681 raised special interest due to their putative role as a toxin–antitoxin system of the mazEF family. Herein, we constructed an S. epidermidis mutant lacking the mazEF genes homologues and determined their role in (i) VBNC state induction during biofilm formation, (ii) antimicrobial susceptibility, (iii) survival in human blood and plasma, and (iv) activation of immune cells. Our results revealed that mazEF homologue did not affect the proportion of VBNC cells in S. epidermidis 1457, refuting the previous hypothesis that mazEF homologue could be linked with the emergence of VBNC cells in S. epidermidis biofilms. Additionally, mazEF homologue did not seem to influence key virulence factors on this strain, since its deletion did not significantly affect the mutant biofilm formation capacity, antimicrobial tolerance or the response by immune cells. Surprisingly, our data suggest that mazEF does not behave as a toxin–antitoxin system in S. epidermidis strain 1457, since no decrease in the viability and culturability of bacteria was found when only the mazF toxin homologue was being expressed.


Author(s):  
Fernando Oliveira ◽  
Tânia Lima ◽  
Alexandra Correia ◽  
Ana Margarida Silva ◽  
Cristina Soares ◽  
...  

Staphylococcus epidermidis is one of the most important nosocomial pathogens and a major cause of central line-associated bloodstream infections. Once in the bloodstream, this bacterium must surpass severe iron restriction in order to survive and establish infection.


Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 468
Author(s):  
Ewelina Namiecińska ◽  
Magdalena Grazul ◽  
Beata Sadowska ◽  
Marzena Więckowska-Szakiel ◽  
Paweł Hikisz ◽  
...  

To meet the demand for alternatives to commonly used antibiotics, this paper evaluates the antimicrobial potential of arene-ruthenium(II) complexes and their salts, which may be of value in antibacterial treatment. Their antimicrobial activity (MIC, MBC/MFC) was examined in vitro against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Pseudomonas aeruginosa, Proteus vulgaris and Candida albicans and compared with classic antibiotics used as therapeutics. Selected arene-ruthenium(II) complexes were found to have synergistic effects with oxacillin and vancomycin against staphylococci. Their bactericidal effect was found to be associated with cell lysis and the ability to cut microbial DNA. To confirm the safety of the tested arene-ruthenium(II) complexes in vivo, their cytotoxicity was also investigated against normal human foreskin fibroblasts (HFF-1). In addition, the antioxidant and thus pro-health potential of the compounds, i.e., their nonenzymatic antioxidant capacity (NEAC), was determined by two different methods: ferric-TPTZ complex and DPPH assay.


2022 ◽  
Vol 21 (1) ◽  
pp. 44-60
Author(s):  
Amir S.R. Al-Obaidil ◽  
Mohammed J. Alwan ◽  
Bushrah ‘ I. Al-Khaisi

Testes from 700 male goats aged l - 3 years , slaughtered at Baghdad abatteir, were examined during 6 months period. Histological and bacteriological examinations were done when gross lesions were observed. The results revealed that epidedimytis was observed in 21 (3%) of the examined animals ; 16 (2.3%) cases were unilaterally affected and S (0.7%) cases were bilaterally affected. Bacteriological isolations from infected organs included : Corynebacterium ovis (6 isolates) and Actinomyces pyogenes (3 isolates) both constituted the majonty of isolates ; as well as E. coli (4 iso-lates), and Pseudomonas aeruginosa , Staphylococcus epidermidis, Cory. bovis., Staph. aureus, Cory , ulcerams &. Yersinia pseuclotuberculosis ( 2 isolates of each ) and Campylobacter fetus (1 isolate). Histopathological examination showed 6 pathological patterns of epididmytis including acute epididymitis (14.2% of infected cases ) , subacute nomsuppurative epididymilis (9.5% od cases ) , chronic suppurative epididymitisd (28.5% of cases ), chronic non—suppurative epididymitis (19% of cases) and spermatic granuloma (14.2% of cases ).


2022 ◽  
Vol 16 (1) ◽  
pp. 4-17

La sepsis neonatal es una importante causa de morbimortalidad. Se realizó un proceso investigativo con el objetivo de describir la etiología y el patrón de susceptibilidad antimicrobiana de las bacterias aisladas más frecuentemente de los hemocultivos de neonatos con sepsis en el servicio de neonatología del Hospital General Docente de Ambato. La metodología empleada en esta investigación se basó en un estudio descriptivo, transversal y enfoque cuali-cuantitativo, empleando la técnica documental y el reporte de resultados como instrumento. De los 39 pacientes estudiados 64,10% fueron del género masculino. El 23,07% presentaron bajo peso y 33,33% una edad gestacional <37 semanas. El microorganismo más frecuente fue Staphylococcus epidermidis (51,28%) seguido de Escherichia coli (17,94%) y Staphylococcus aureus (15,38%). En relación al perfil de susceptibilidad antimicrobiana S. epidermidis y S. aureus se mostraron sensibles a linezolid y vancomicina en más del 80,00%, y presentaron alta resistencia a oxacilina (80,00 y 83,33%, respectivamente), estas cepas expresaron fenotípicamente el gen mecA. Las enterobacterias aisladas mostraron resistencia a amoxacilina/ácido clavulánico (61,53%), ampicilina/sulbactam (69,23%), ciprofloxacina (61,53%), ceftazidima (30,76%) y cefotaxima (38,46%). Además, cinco cepas de E. coli y Klebsiella pneumoniae eran fenotípicamente productoras de beta lactamasas de espectro extendido. En conclusión, es necesario realizar estudios locales de vigilancia microbiológica en los hospitales, con el fin de identificar los patógenos multirresistentes involucrados en las infecciones neonatales, reconocer los brotes y monitorizar los cambios que ocurren a través del tiempo; los cuales influyen finalmente, en la elección de los tratamientos empíricos.


2022 ◽  
Vol 508 (1) ◽  
Author(s):  
Trần Huy Cường ◽  
Huỳnh Minh Tuấn ◽  
Hoàng Tiến Mỹ ◽  
Lý Khánh Vân

Đặt vấn đề: Staphylococcus epidermidis (S. epidermidis) là tác nhân gây bệnh nhiễm khuẩn cơ hội, thường gặp trên da, niêm mạc của bệnh nhân khi được thực hiện can thiệp thủ thuật ngoại khoa. Các nghiên cứu cho thấy, tình trạng kháng kháng sinh của S. epidermidis ngày một nghiêm trọng. Mục tiêu: xác định tỉ lệ S. epidermidis phân lập được trên vùng da rốn và bẹn ở bệnh nhân ngay trước phẫu thuật và tỉ lệ S. epidermidis đề kháng một số kháng sinh thường dùng. Phương pháp nghiên cứu: từ 218 bệnh nhân tham gia nghiên cứu, lấy bệnh phẩm quệt da bằng tăm bông vô khuẩn tại vùng da rốn hoặc bẹn tại ba thời điểm: sau khi bệnh nhân được tắm và gây mê (lần 1), sau khi điều dưỡng rửa da (lần 2), sau khi phẫu thuật viên sát khuẩn da (lần 3), thu thập được 654 bệnh phẩm. Tiến hành nuôi cấy, định danh bằng bộ trắc nghiện sinh hóa dành cho Staphylococci và hệ thống tự động BD PhoenixTM M50, kháng sinh đồ bằng phương pháp khuếch tán đĩa giấy với 654 bệnh phẩm trên. Kết quả: tỉ lệ S. epidermidis tại vùng da rốn ở bệnh nhân ngay trước phẫu thuật qua ba thời điểm lấy bệnh phẩm lần lượt là: 33,1% (lần 1); 10,2% (lần 2) và 1,8% (lần 3) và tỉ lệ có S. epidermidis tại vùng da bẹn ở bệnh nhân ngay trước phẫu thuật qua ba thời điểm lấy bệnh phẩm lần lượt là: 32,7% (lần 1); 9,6% (lần 2) và 1,9% (lần 3). Tỉ lệ đề kháng kháng sinh của S. epidermidis lần lượt qua ba thời điểm lấy bệnh phẩm: penicillin (83,3%; 95,5% và 100%); erythromycin (72,2%; 86,4% và 100%); oxacillin (58,3%; 59,1% và 75%); trimethoprim-sulfamethoxazole (36,1%; 59,1% và 75%); ciprofloxacin (30,6; 36,4 và 0%); clindamycin (16,7%; 22,7% và 50%); levofloxacin (22,2%; 22,7% và 0%); tetracycline (19,4%; 22,7% và 0%); gentamycin (20,8%; 13,6% và 25%); doxycycline (1,4%; 0% và 0%). Riêng với linezolid: 100% các chủng S. epidermidis phân lập được đều nhạy cảm. Kết luận: tỉ lệ phát hiện S. epidermidis ở vùng da rốn và bẹn sau khi sát khuẩn da lần 3 là 1,8% và 1,9%. S. epidermidis kháng với nhiều loại kháng sinh, từ 75-100% đối với penicillin, erythromycin, oxacillin nhưng còn nhạy cảm với linezolid.


Author(s):  
Livia Pagano ◽  
Foteini Gkartziou ◽  
Stefano Aiello ◽  
Beatrice Simonis ◽  
Francesca Ceccacci ◽  
...  

2021 ◽  
Vol 57 (4) ◽  
pp. e208
Author(s):  
Greta Tavarez-Martínez ◽  
Belén Criado ◽  
M. Coronada Fernández-Calderón ◽  
Edgar Onofre-Bustamante ◽  
Ciro Pérez-Giraldo ◽  
...  

El objetivo de este trabajo ha sido estudiar si los tratamientos térmicos y de conversión química mejoran la biocompatibilidad de la aleación TiAlV y reducen el crecimiento bacteriano. En primer lugar, se modificó la aleación de TiAlV mediante tratamiento térmico a 650 ºC durante 1 hour. Luego, se llevó a cabo la conversión química en una solución de CeCl3 para generar óxido de cerio. Las superficies modificadas se caracterizaron utilizando AFM y SEM-EDX. La adhesión de osteoblastos y la formación de biopelículas microbianas se midieron in vitro con la línea celular de osteoblastos MC3T3-E1 y Staphylococcus epidermidis ATCC 35983, respectivamente. La viabilidad bacteriana se cuantificó a través del contenido en trifosfato de adenosina (ATP) como medida de la actividad metabólica. La morfología y la proliferación en superficies modificadas se analizaron mediante SEM-EDX. Los resultados revelaron que el TiAlV tratado térmicamente mostró una mayor proliferación osteoblástica asociada con una mayor rugosidad y estructura cristalina del rutilo. Las superficies modificadas no causaron efecto bactericida, pero las superficies de TiAlV con ceria mostraron una disminución en la adhesión bacteriana, es decir, menos proliferación bacteriana y por tanto disminución en la colonización bacteriana.


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