8F-08 Determination of blood coagulation factors during in vitro antithrombogenic testing for a centrifugal blood pump

The influence of blood coagulation factors in pat. with acute respiratory insufficiency of adults, especially of the so called “pancreatitis lungs” is still unknown. In order to find out the effect of elastase, possibly activated by trypsin in pat. with acute pancreatitis, on blood coagulation factors, we performed some studies. In vitro elastase induces in plasma and blood in correlation to the dosages Enhancement of thrombingeneration in the TGT, a shortening of PTT, Thrombin time and of r- and k-time in the TEG, a loss of fibrinogen and an increase of fibrinmono-mercomplexes. In another study, elastase (960 U/ kg b.w.) was injected intravenously in rats. 30 min. later there was found a loss of fibrinogen, number of platelets, Prothrombin and a prolongation of PTT and Thrombin time and an increase of fibrinomonomercomplexes, especially in these rats, which received beside elastase Kalikreininhibitors or antifibrinolytic drugs. After repeated injections (3 times within 30 h) we found histomorpholgically thrombi as well as bleeding complications. In another study we performed (150 min) an infusion of elastase (333 U/kg b.w./h) to 9 pigs. We determined a loss of fibrinogen of platelets, of F. II, F. VII and F. XIII, a prolongation of PTT. F. VIII and F. V remained within the normal range But there was found an enhancement of Thrombin generation in the TGT, too. Compariening the results of blood coagulation tests and of histomorphological findings, elastase induced a DIC. We have to discuss their influence on ARIA and “Pancreatic lungs”.

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Inhibitors of blood coagulation factors Xa and IIa synergize to reduce thrombus weight and thrombin generation in vivo and in vitro

Journal of Thrombosis and Haemostasis ◽

10.1111/j.1538-7836.2006.01830.x ◽

2006 ◽

Vol 4 (4) ◽

pp. 834-841 ◽

Cited By ~ 14

Author(s):

W. R. GOULD ◽

T. B. MCCLANAHAN ◽

K. M. WELCH ◽

S. M. BAXI ◽

K. SAIYA-CORK ◽

...

Keyword(s):

Blood Coagulation ◽

Thrombin Generation ◽

Coagulation Factors ◽

Blood Coagulation Factors

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Natural Oestrogen in the Female Climacteric - Influence on Blood Coagulation and Fibrinolysis

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648686 ◽

1978 ◽

Vol 40 (02) ◽

pp. 532-541 ◽

Cited By ~ 2

Author(s):

Anders Lagrelius ◽

Nils-Olov Lunell ◽

Margareta Blombäck

Keyword(s):

Blood Coagulation ◽

Antithrombin Iii ◽

Coagulation Factors ◽

Control Group ◽

Blood Coagulation Factors ◽

Excessive Bleeding ◽

Oestrone Sulphate ◽

Menopausal Women ◽

History Of ◽

Coagulation And Fibrinolysis

SummaryThe aim of the present study was to investigate the effect on blood coagulation and fibrinolysis of a natural oestrogen preparation, piperazine oestrone sulphate, prospectively in menopausal women. Scopolamine was given to the control group.The women were investigated before and during treatment with regard to factors VIII, VII, X, V, fibrinopeptide A, antithrombin III, plasminogen, rapid antiplasmin and α1-antitrypsin. There was no significant change towards hypercoagulability or decreased fibrinolysis in any group. In the oestrogen group, however, a tendency towards an increased level of plasminogen and a decreased level of antiplasmin was demonstrated. In the scopolamine group there was an unexpected fall in factors X and V and also in plasminogen and α1,-antitrypsin. A low level of some blood coagulation factors in some of the women before treatment is somewhat astonishing; none of them had any history of excessive bleeding.

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Factor II Related Antigen and Antithrombin III Levels as Indicators of Liver Failure in Consumption Coagulopathy

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657171 ◽

1982 ◽

Vol 47 (03) ◽

pp. 218-220 ◽

Cited By ~ 13

Author(s):

P Sié ◽

E Letrenne ◽

C Caranobe ◽

M Genestal ◽

B Cathala ◽

...

Keyword(s):

Liver Failure ◽

Antithrombin Iii ◽

Coagulation Factors ◽

Consumption Coagulopathy ◽

Blood Coagulation Factors ◽

Factor Ii ◽

At Iii ◽

Group A ◽

Group B

SummaryIn order to detect impaired synthesis of blood coagulation factors associated to consumption coagulopathy, a simultaneous evaluation of factor II-related antigen (II rAg) and of antithrombin III (AT III) was carried out in 16 patients affected with severe defibrination. An in vitro preliminary study on plasma and serum demonstrated that the levels of II rAg and of AT III, assessed by the Laurell technique with Behring antisera, were not reduced by the coagulation process. The patients were, a posteriori, classified into two groups according to the absence (group A) or the presence (group B) of factors predisposing to liver failure such as metastasis, cirrhosis, and prolonged shock. II rAg and AT III levels are significantly correlated; they are in the normal range in group A but reduced in group B. Thus II rAg or AT III level determinations are useful markers in the detection of liver failure associated to the consumption phenomenon. These results also suggest that part of the decreased AT III levels reported in severe cases of disseminated intravascular coagulation may be the consequence of an associated liver failure.

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STEROIDSTRUKTUR UND LEBERTOXIZITÄT

Acta Endocrinologica ◽

10.1530/acta.0.0540073 ◽

1967 ◽

Vol 54 (1) ◽

pp. 73-84 ◽

Cited By ~ 7

Author(s):

H. L. Krüskemper ◽

G. Noell

Keyword(s):

Alkaline Phosphatase ◽

Liver Function ◽

Blood Coagulation ◽

Electrophoretic Pattern ◽

Coagulation Factors ◽

Blood Coagulation Factors ◽

Methyl Group ◽

Liver Functions ◽

Male Subjects ◽

Androstane Derivatives

ABSTRACT In male subjects investigations have been carried out regarding the effect of C1- and C17-methylated androstane derivatives (20 mg per day, orally, two weeks) on liver functions (parameters: activities of GPT, GOT, alkaline phosphatase and cholinesterase in serum; electrophoretic pattern; blood coagulation factors V, VII, X and prothrombin; BSP-retention). In addition to the well known hepatotropic action of 17α-alkylated C-19-steroids a quasi-axial 1α-methyl configuration (in 1α-methylandrost-2-en-17β-ol) definitely increased BSP-retention and several coagulation factors. These steroid effects decreased gradually when a methyl group was introduced in C1 equatorially (1-methylandrost-1-en-17β-ol-3-one) or quasi-equatorially (1β-methylandrost-2-en-17β-ol), the latter compound completely lacking from any influence on parameters of liver function under investigation.

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Localization of the structural difference between bovine blood coagulation factors X1 and X2 to tyrosine 18 in the activation peptide.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)35614-4 ◽

1986 ◽

Vol 261 (9) ◽

pp. 4008-4014 ◽

Cited By ~ 2

Author(s):

T Morita ◽

C M Jackson

Keyword(s):

Blood Coagulation ◽

Structural Difference ◽

Coagulation Factors ◽

Bovine Blood ◽

Blood Coagulation Factors ◽

Activation Peptide

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EXPRESSION IN ONTOGENESIS OF HUMAN BLOOD COAGULATION FACTORS

10.1055/s-0038-1644610 ◽

1987 ◽

Author(s):

H J Hassan ◽

A Leonardi ◽

C Chelucci ◽

R Guerriero ◽

P M Mannucci ◽

...

Keyword(s):

Blood Coagulation ◽

Protein C ◽

Antithrombin Iii ◽

Liver Homogenate ◽

Coagulation Factor ◽

Coagulation Factors ◽

Factor Ix ◽

Adult Liver ◽

Blood Coagulation Factors ◽

Guanidine Isothiocyanate

We have analyzed the expression of several blood coagulation factors (IX, VIII, X, fibrinogen chains) and inhibitors (antithrombin III, protein C) in human embryonic and fetal livers, obtained from legal abortions at 6-11 week post-conception. The age was established by morphologic staging and particularly crown-rump lenght measurement.Total cellular RNA was isolated from partially purified hepatocytes or total liver homogenate using the guanidine isothiocyanate method. Poly(A)+ RNA was selected by oligodT cellulose chromatography. The size and the number of the embryonic and fetal transcripts are equivalent to those observed in adult liver, as evaluated by Northern blot analysis of total or poly(A)+ RNA hybridized to human cDNA probes.The level of coagulation factor transcripts in embryonic and fetal liver was evaluated by dot hybridization of total RNA (0.5-10 ug), as compared to RNA extracted from normal adult liver biopsies. The expression of blood coagulation factors in embryos is generally reduced for all factors, but at a different degree. In 5-11 wk liver, the level of factor IX is 5-10% of that observed in adults, while fibrinogen, protein C, antithrombin III RNA level rises from 25 to 50% and factor X is expressed at a level comparable to that observed in adult liver.We conclude that during these stages of development blood coagulation factors are expressed according to three different time, curves, possibly due to the effect of different types of regulatory mechanisms.

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