scholarly journals Screening for Isolation of Succinic Acid Producing Microbes

2018 ◽  
Vol 15 (2) ◽  
pp. 327-333 ◽  
Author(s):  
Hema N ◽  
K. P. Sreenath
Keyword(s):  
Organic Acids ◽  
Thin Layer ◽  
Succinic Acid ◽  
Thin Layer Chromatography ◽  
Acid Production ◽  
Colour Change ◽  
Bromocresol Green ◽  
Succinic Acid Production ◽  
Layer Chromatography

Succinic acid has emerged as one of the most competitive bio-based chemicals. Present study intended to isolate potential strains for the production of succinic acid. More than 90 isolates were obtained from various sources using anaerobic techniques. All the strains were screened for succinic acid production through bromocresol green media, thin layer chromatography and HPLC methods. 74 isolates showed colour change in bromocresol green media indicating the production of organic acids, of which 14 isolates showed succinic acid production determined by thin layer chromatography which were quantified through HPLC. The isolates which yielded succinic acid ranged from 0.24g/l (MGC) to 4.69g/l (RFC-P3).

scholarly journals The use of Bacillus subtilis for screening fusaric acid production by Fusarium spp.

2009 ◽  
Vol 27 (No. 3) ◽  
pp. 203-209 ◽  
Author(s):  
A. Šrobárová ◽  
Š. Eged ◽  
J. Teixeira Da Silva ◽  
A. Ritieni ◽  
A. Santini
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Screening Test ◽  
Acid Production ◽  
Fusaric Acid ◽  
Modified Method ◽  
Layer Chromatography

Fusaric acid (FA) is one of the most important secondary metabolites produced by <I>Fusarium oxysporum</I> (Schlecht) (FO), <I>F. solani</I> (Mart.) Appel & Wollenweber, and <I>F. moniliforme</I> Sheldon. It is toxic to humans, many plants, and microorganisms and it enhances the toxicity of fumonisin and trichothecene. A simple and rapid method for fusaric acid (FA) screening in <I>Fusarium</I> isolates was developed. In this study, several strains of <I>Fusarium oxysporum</I> were tested for their ability to produce FA by using a suitable race of <I>Bacillus subtilis</I> as the bioassay. A modified method using small agar blocks with the fungus producing FA was applied in the screening test. FA standard and <I>F. culmorum</I> were used as controls. The experimental <I>F. oxysporum</I> isolates and FA standard produced transparent zones on the plates with <I>Bacillus subtilis</I>. The differences in size of the transparent zones corresponded to the quantity of FA when thin-layer chromatography was used.

Inhibition of succinic acid production in metabolically engineeredEscherichia coliby neutralizing agent, organic acids, and osmolarity

2009 ◽  
Vol 25 (1) ◽  
pp. 116-123 ◽  
Author(s):  
Christian Andersson ◽  
Jonas Helmerius ◽  
David Hodge ◽  
Kris A. Berglund ◽  
Ulrika Rova
Keyword(s):  
Organic Acids ◽  
Succinic Acid ◽  
Acid Production ◽  
Succinic Acid Production ◽  
Neutralizing Agent

Thin-Layer Chromatography of Organic Acids

1973 ◽  
Vol 51 (3) ◽  
pp. 318-320 ◽  
Author(s):  
A. R. Beaudoin ◽  
S. Moorjani ◽  
A. Lemonde
Keyword(s):  
Citric Acid ◽  
Organic Acids ◽  
Thin Layer ◽  
Magnesium Sulfate ◽  
Thin Layer Chromatography ◽  
Citric Acid Cycle ◽  
Acid Cycle ◽  
Clear Separation ◽  
Reproducible Method ◽  
Layer Chromatography

A simple and reproducible method is described for the separation of citric acid cycle intermediates and other related organic acids. The separation is achieved on thin-layer plates (T.L.C.) coated with silica gel H to which magnesium sulfate and sodium carbonate are added. This T.L.C. method ensures clear separation of organic acids. A procedure is also described that permits quantitative recovery of the radioactivity present in citric, glyoxylic, and isocitric acids.

Cyclopiazonic Acid Production by Cultures of Aspergillus and Penicillium Species Isolated from Dried Beans, Corn Meal, Macaroni, and Pecans

1987 ◽  
Vol 70 (1) ◽  
pp. 123-126 ◽  
Author(s):  
Mary W Trucksess ◽  
Philip B Mislivec ◽  
Kathryn Young ◽  
Verneal R Bruce ◽  
Samuel W Page
Keyword(s):  
Thin Layer ◽  
Liquid Medium ◽  
Thin Layer Chromatography ◽  
Acid Production ◽  
Cyclopiazonic Acid ◽  
Corn Meal ◽  
Penicillium Species ◽  
Visual Comparison ◽  
Layer Chromatography ◽  
Dried Foods

Abstract Ninety-five isolates of Aspergillus and Penicillium species from selected dried foods were examined for their ability to produce cyclopiazonic acid (CPA). The isolates were grown in sterile synthetic liquid medium at 28°C for 8 days in the dark. The medium and mold mycelia were then extracted with chloroform. CPA was semiquantitative^ determined by thin layer chromatography through visual comparison with standards. The cultures of A. flavus were also examined for their ability to produce aflatoxin. One A. tamarii and all 13 P. urticae isolates produced CPA, whereas only 19 of the 31 (61%) A. flavus isolates produced CPA, and 6 (19%) A. flavus produced aflatoxin. All 13 P. urticae isolates also produced patulin and griseofulvin. CPA-producing A. flavus was found in all food types but not in all samples. CPA-producing P. urticae was found only in dried beans and macaroni.

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