scholarly journals The use of Bacillus subtilis for screening fusaric acid production by Fusarium spp.

2009 ◽  
Vol 27 (No. 3) ◽  
pp. 203-209 ◽  
Author(s):  
A. Šrobárová ◽  
Š. Eged ◽  
J. Teixeira Da Silva ◽  
A. Ritieni ◽  
A. Santini
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Screening Test ◽  
Acid Production ◽  
Fusaric Acid ◽  
Modified Method ◽  
Layer Chromatography

Fusaric acid (FA) is one of the most important secondary metabolites produced by <I>Fusarium oxysporum</I> (Schlecht) (FO), <I>F. solani</I> (Mart.) Appel & Wollenweber, and <I>F. moniliforme</I> Sheldon. It is toxic to humans, many plants, and microorganisms and it enhances the toxicity of fumonisin and trichothecene. A simple and rapid method for fusaric acid (FA) screening in <I>Fusarium</I> isolates was developed. In this study, several strains of <I>Fusarium oxysporum</I> were tested for their ability to produce FA by using a suitable race of <I>Bacillus subtilis</I> as the bioassay. A modified method using small agar blocks with the fungus producing FA was applied in the screening test. FA standard and <I>F. culmorum</I> were used as controls. The experimental <I>F. oxysporum</I> isolates and FA standard produced transparent zones on the plates with <I>Bacillus subtilis</I>. The differences in size of the transparent zones corresponded to the quantity of FA when thin-layer chromatography was used.

scholarly journals Secondary Metabolites Production by Actinomycetes and their Antifungal Activity

2017 ◽  
Vol 3 (4) ◽  
pp. 256 ◽  
Author(s):  
Tirta Kumala Dewi ◽  
Dwi Agustiani ◽  
Sarjiya Antonius
Keyword(s):  
Antifungal Activity ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Wide Range ◽  
Layer Chromatography

<p class="Els-Abstract-text">Wilt desease of banana caused by <em>Fusarium oxysporum</em> f.sp. <em>cubense</em> (FOC) is one of the most destructive deseases of banana in the tropics. Actinomycetes are the most economically and biotechnologically valuable prokaryotes able to produce wide range of bioactive secondary metabolites. The aims of the present study are to isolate and screen the actinomycetes with high potential ability to produce secondary metabolites that have inhibitory activity against plant pathogenic fungi, <em>Fusarium oxysporum</em> f. sp. <em>cubense</em>. Two isolates from Lampung and Cianjur showed activity against fungi. The isolates designed as L.3.1 and CiIA5b. The metabolites from potent stain was produced by extraction of culture filtrate with ethyl acetate : methanol (4:1), it was tested for their antifungal activity by well diffusion method. Evidence for in vitro antibiosis of L.3.1 and CiIA5b isolates was demonstrated by the zone of fungal-growth inhibition. Production of secondary metabolites was analysis by thin layer chromatography (TLC) and bioautography assays. In this study, the metabolites from L.3.1 and CiIA5b have showed good antifungal activity.</p><div><p class="Els-keywords"><em> </em></p><p class="Els-keywords"><strong>Keywords:</strong> Actinomycetes; antifungal activity; bioautography; secondary metabolites; thin layer chromatography.</p></div>

scholarly journals Screening for Isolation of Succinic Acid Producing Microbes

2018 ◽  
Vol 15 (2) ◽  
pp. 327-333 ◽  
Author(s):  
Hema N ◽  
K. P. Sreenath
Keyword(s):  
Organic Acids ◽  
Thin Layer ◽  
Succinic Acid ◽  
Thin Layer Chromatography ◽  
Acid Production ◽  
Colour Change ◽  
Bromocresol Green ◽  
Succinic Acid Production ◽  
Layer Chromatography

Succinic acid has emerged as one of the most competitive bio-based chemicals. Present study intended to isolate potential strains for the production of succinic acid. More than 90 isolates were obtained from various sources using anaerobic techniques. All the strains were screened for succinic acid production through bromocresol green media, thin layer chromatography and HPLC methods. 74 isolates showed colour change in bromocresol green media indicating the production of organic acids, of which 14 isolates showed succinic acid production determined by thin layer chromatography which were quantified through HPLC. The isolates which yielded succinic acid ranged from 0.24g/l (MGC) to 4.69g/l (RFC-P3).

scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN MAHONI (Swietenia mahagoni (L.) Jacq.) TERHADAP LARVA Aedes aegypti L.

2017 ◽  
Vol 4 (2) ◽  
pp. 23
Author(s):  
Tisa Rizkika Nur Amelia
Keyword(s):  
Secondary Metabolites ◽  
Aedes Aegypti ◽  
Thin Layer ◽  
Key Words ◽  
Thin Layer Chromatography ◽  
Ethanolic Extract ◽  
Botanical Insecticide ◽  
Flavonoid Compounds ◽  
Layer Chromatography ◽  
Swietenia Mahagoni

<p>The aim of the research were to evaluate the efficacy of botanical insecticide of <em>S. mahagoni </em>leaves extracts against larvae of <em>Ae. aegypti,</em> based on concentration of the leaves <em>S. mahagoni </em>extract, and in additional to determine secondary metabolites compounds of <em>S. mahagoni </em>leaves extract. The extraction of <em>S. mahagoni </em>leaves was done by using ethanol solvents and than was analyzed by using Thin Layer Chromatography. The result indicated that ethanolic extract of <em>S. mahagoni </em>leaf contained alkaloid, tannin, saponin, terpenoid, and flavonoid compounds. The value of LC<sub>50 </sub>and LC<sub>90</sub> calculation showed that LC<sub>50</sub> of ethanolic extract over second and third instar larvae respectively were 488 ppm and 644 ppm. However the value of LC<sub>90</sub> of both instar larvae were 732 ppm and 797 ppm. Based on the above result, it can be concluded that ethanolic extract of <em>S. mahagoni </em>leaf was effective against larvae of <em>Ae .aegypti</em>.</p><p>Key words: <em>Ae. aegypti</em>, <em>S. mahagoni</em>, botanical insecticide</p>

TELAAH FITOKIMIA KULIT KACANG TANAH (Arachis hypogaea, L).

2021 ◽  
Vol 10 (1) ◽  
pp. 82-91
Author(s):  
Rizki Nisfi Ramdhini ◽  
Isna Mulyani ◽  
Syaikhul Aziz
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Waste Product ◽  
Phytochemical Screening ◽  
Medicinal Products ◽  
Processing Industry ◽  
Arachis Hypogaea L ◽  
Tlc Analysis ◽  
Layer Chromatography

Peanut peel are a waste product of the peanut processing industry with little commercial value. Some of studies have been conducted indicating peanut peel can be beneficial as a source for traditional medicinal products since it is also rich of antioxidants. The aim of this research was to identify the content of secondary metabolites on the peanut peel. The method used was maseration with 96% ethanol. Phytochemical screening and assaying were performed using thin layer chromatography (TLC) method. The results of TLC analysis showed that the secondary metabolites in peanut peel were positive for flavonoids, alkaloids, tannins and quinon. Keywords: Peanut peel, Phytochemical, Thin-Layer chromatography (TLC)

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