scholarly journals Hepatorenal Effects of Silver Nanoparticles in In-Vivo Postnatal Model of Toxicity and in HepG2 Cell Line

2020 ◽  
Vol 17 (Issue 1) ◽  
pp. 54-61
Author(s):  
Mahmoud M. Elalfy ◽  
Mamdouh Abouelmagd ◽  
Eman A. Abdelraheem ◽  
Mona G. El-hadidy
Keyword(s):  
Silver Nanoparticles ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Female Rats ◽  
Hepg2 Cell Line ◽  
Ag Nps ◽  
Hepatic Cells ◽  
Mouse Liver Cell ◽  
Liver And Kidney

Silver nanoparticles (Ag-NPs) had many uses in medicine, household and industry. To better understand the postnatal toxicity of Ag-NPs in lactating female rats and its offspring’s, 18 female rats after delivery were divided into three groups and dams received orally the AG-NPs at doses of 0, 50, 100 ppm daily for 21 days. After the end of treatment, all rats were euthanized and blood and tissues were separated for evaluation of biochemical and histopathology in dams and its pups. The Ag-NPs had no effect on the dam's weight while the reduction of rats’ pups weight was noticed after first week only after the treatment. Notably, Ag-NPs had toxic effects in rat’s pups, as well as its dam with evidence of elevation of liver enzymes, urea, creatinine and reduction of serum protein, albumin and globulin and considered the first report explained the toxicity in the rat’s pups. Moreover, rats' pups revealed histopathological changes in liver and kidney as well as its dams. Notably, the nano-silver is considered cytotoxic for HepG2 cell line as well as mouse liver cell line. In conclusions, the Ag-NPs considered toxic in offspring as well as dams and had immunosuppressive effects in the postnatal model of toxicity as well as cytotoxicity to hepatic cells lines.

scholarly journals The effect of different doses levels of silver nanoparticles (AgNPs) on the kidney and liver in Albino male Rat. Histopathological study

2014 ◽  
Vol 11 (4) ◽  
pp. 1503-1509
Author(s):  
Baghdad Science Journal
Keyword(s):  
Silver Nanoparticles ◽  
Body Weight ◽  
Male Rat ◽  
Central Vein ◽  
Average Particle ◽  
Ag Nps ◽  
Hepatic Cells ◽  
Liver And Kidney ◽  
Collecting Tubules ◽  
Convoluted Tubules

Objective: In this study ,the effects of silver nanoparticles (Ag NPs)were investigated on the liver and kidney tissues. Methodology: The produced nanoparticles have an average particle size of about 30 nm. Eighteen male albino rats were used by dividing them into three groups, each group comprise 6 rats. First group(control group) given food and water like other groups by liberty. Second group was tail injected by (AgNPs) at dose of (0.4 mg/kg. body weight/day). Third group was injected by (AgNPs) at dose of (0.6 mg/kg. body weight/day) for 15 days. All animals were sacrified at the end of experiment. The liver and kidney tissues specimens were fixed in 10% formalin and histological preparations were carried out then stained with H&E. Pathological changes in liver and kidney tissues were showed. Results: Histopathological studies revealed the harmful effect of the silver nanoparticles uses on the liver and kidney rats, second group that treated with Ag NPs (0.4 mg/kg.body.weight/day), kidney sections showed enlargement of collecting tubules, increase in interstitial tissue medulla, necrosis and enlargement in proximal and distal convoluted tubules. Liver showed enlargement of the central vein and degeneration of hepatic cells. Third group that treated with Ag NPs (0.6 mg/kg. body weight/day); kidney sections showed hyperplasia of the interstitial connective tissue of renal medulla with hemorrhages, renal cortex showed, degenerative changes and necrosis of proximal and distal convoluted tubules. Liver section showed congestion and necrosis of hepatic cells. Conclusion: Silver nanoparticles cause damage in liver and kidney tissues. Recommendation: Further study is needed for the effect of Ag NPs on the other tissues.

Preparation of stabilized silver nanoparticles and study of their antimicrobial and cytotoxic activity on the human hepatoma HepG2 cell line

2019 ◽  
Vol 14 (5-6) ◽  
pp. 273-279
Author(s):  
M. A. Ananyan ◽  
A. G. Demchenko ◽  
V. S. Sadykova ◽  
A. V. Lyundup ◽  
T. I. Gromovykh ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Human Hepatoma ◽  
Hepg2 Cell Line ◽  
Hepatoma Hepg2

scholarly journals Characterization of In Vitro 3D Cell Model Developed from Human Hepatocellular Carcinoma (HepG2) Cell Line

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2557
Author(s):  
Martina Štampar ◽  
Barbara Breznik ◽  
Metka Filipič ◽  
Bojana Žegura
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Cell Model ◽  
Human Hepatocellular Carcinoma ◽  
Time Dependent ◽  
Hepg2 Cell Line ◽  
Cell Models

In genetic toxicology, there is a trend against the increased use of in vivo models as highlighted by the 3R strategy, thus encouraging the development and implementation of alternative models. Two-dimensional (2D) hepatic cell models, which are generally used for studying the adverse effects of chemicals and consumer products, are prone to giving misleading results. On the other hand, newly developed hepatic three-dimensional (3D) cell models provide an attractive alternative, which, due to improved cell interactions and a higher level of liver-specific functions, including metabolic enzymes, reflect in vivo conditions more accurately. We developed an in vitro 3D cell model from the human hepatocellular carcinoma (HepG2) cell line. The spheroids were cultured under static conditions and characterised by monitoring their growth, morphology, and cell viability during the time of cultivation. A time-dependent suppression of cell division was observed. Cell cycle analysis showed time-dependent accumulation of cells in the G0/G1 phase. Moreover, time-dependent downregulation of proliferation markers was shown at the mRNA level. Genes encoding hepatic markers, metabolic phase I/II enzymes, were time-dependently deregulated compared to monolayers. New knowledge on the characteristics of the 3D cell model is of great importance for its further development and application in the safety assessment of chemicals, food products, and complex mixtures.

scholarly journals Cytotoxicity of Silver Nanoparticles in Human Embryonic Stem Cell-Derived Fibroblasts and an L-929 Cell Line

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Hui Peng ◽  
Xuehui Zhang ◽  
Yan Wei ◽  
Wentao Liu ◽  
Shenglin Li ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Line ◽  
Human Embryonic Stem Cell ◽  
Cell Model ◽  
Embryonic Stem ◽  
Ag Nps ◽  
P53 Expression

Consensus about the toxicity of silver nanoparticles (Ag-NPs) has not been reached, even though extensive attention has been paid to this issue. This confusion may be due to physicochemical factors of Ag-NPs and the cell model used for biological safety evaluation. In the present study, human embryonic stem cell-derived fibroblasts (EBFs), which have been considered a closer representative of thein vivoresponse, were used as a novel cell model to assess the cytotoxicity of Ag-NPs (~20 nm and~100 nm) in comparison with L-929 fibroblast cell line. Cell proliferation, cell cycle, apoptosis, p53 expression, and cellular uptake were examined. Results showed that Ag-NPs presented higher cytotoxicity to EBF than to L-929. EBF demonstrated a stronger capacity to ingest Ag-NPs, a higher G2/M arrest, and more upgraduated p53 expression after exposed to Ag-NPs for 48 h when compared with L-929. It could be concluded that EBF exhibited a more sensitive response to Ag-NPs compared with L-929 cells, indicating that EBF may be a valid candidate for cytotoxicity screening assays of nanoparticles.

Effect of silver nanoparticles in the induction of apoptosis on human hepatocellular carcinoma (HepG2) cell line

2018 ◽  
Vol 93 ◽  
pp. 465-471 ◽  
Author(s):  
Elham Ahmadian ◽  
Solmaz Maleki Dizaj ◽  
Elaheh Rahimpour ◽  
Amir Hasanzadeh ◽  
Aziz Eftekhari ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Silver Nanoparticles ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Human Hepatocellular Carcinoma ◽  
Hepg2 Cell Line ◽  
Induction Of Apoptosis

Norbornene derived nanocarrier reduces isoniazid mediated liver toxicity: assessment in HepG2 cell line and zebrafish model

RSC Advances ◽  
2016 ◽  
Vol 6 (115) ◽  
pp. 114927-114936 ◽  
Author(s):  
Thangam Anju ◽  
Radhakrishnan Preetha ◽  
Raja Shunmugam ◽  
Shivshankar R. Mane ◽  
Jesu Arockiaraj ◽  
...  
Keyword(s):  
Protective Effect ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Liver Toxicity ◽  
Toxicity Assessment ◽  
Hepg2 Cell Line ◽  
Stimuli Responsive ◽  
Zebrafish Model

The aim of this study was to investigate the protective effect of the stimuli-responsive norbornene-based nanocarrier complex of isoniazid, compared to pure isoniazid, on liver cells, by in vivo and in vitro methods.

HGF ameliorates a high-fat diet-induced fatty liver

2007 ◽  
Vol 293 (1) ◽  
pp. G204-G210 ◽  
Author(s):  
Takashi Kosone ◽  
Hitoshi Takagi ◽  
Norio Horiguchi ◽  
Yasuyo Ariyama ◽  
Toshiyuki Otsuka ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Fatty Liver ◽  
Cell Line ◽  
Hepg2 Cell ◽  
High Fat Diet ◽  
Normal Diet ◽  
Hepg2 Cell Line ◽  
High Fat

Hepatocyte growth factor (HGF) has various effects especially on epithelial cells. However, the precise role of HGF on lipogenesis is still not fully understood. A high-fat diet was administered to HGF transgenic mice and wild-type control mice in vivo. Furthermore, recombinant human HGF (rhHGF) was administered to HepG2 cell line in vitro. We performed an analysis regarding the factors relating to lipid metabolism. An overexpression of HGF dramatically ameliorates a high-fat diet-induced fatty liver. HGF transgenic mice showed an apparently reduced lipid accumulation in the liver. The activation of microsomal triglyceride transfer protein (MTP) and apolipoprotein B (ApoB) accompanying higher triglyceride levels in the serum were found in HGF transgenic mice on a normal diet. Interestingly, this upregulation of the MTP activation became more apparent in the high-fat diet. In addition, the administration of rhHGF stimulated MTP and ApoB expression while reducing reduced the intracellular lipid content in HepG2 cell line. However, this induction of MTP and ApoB by HGF was clearly inhibited by PD98059 (MAPK inhibitor). In conclusion, the data presented in this study indicated that HGF ameliorates a high-fat diet-induced fatty liver via the activation of MTP and ApoB.

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