Comparison of Four Enzyme Immunoassays With a Western Blot Assay for the Determination of Type-Specific Antibodies to Herpes Simplex Virus

2001 ◽  
Vol 115 (2) ◽  
pp. 272-277 ◽  
Author(s):  
Thomas B. Martins ◽  
Ryan D. Woolstenhulme ◽  
Troy D. Jaskowski ◽  
Harry R. Hill ◽  
Christine M. Litwin
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Western Blot ◽  
Western Blot Assay ◽  
Specific Antibodies ◽  
Enzyme Immunoassays ◽  
Simplex Virus

The glycoproteins C and G are equivalent target antigens for the determination of herpes simplex virus type 1-specific antibodies

2010 ◽  
Vol 166 (1-2) ◽  
pp. 42-47 ◽  
Author(s):  
Thomas Scheper ◽  
Sandra Saschenbrecker ◽  
Katja Steinhagen ◽  
Andreas Sauerbrei ◽  
Waltraud Suer ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Specific Antibodies ◽  
Simplex Virus

scholarly journals Performance of the Euroline Western blot assay in the detection of herpes simplex virus type 2 antibody in Uganda, China and the USA

2011 ◽  
Vol 22 (6) ◽  
pp. 342-344 ◽  
Author(s):  
J D Neal ◽  
A A R Tobian ◽  
O Laeyendecker ◽  
T D Ngo ◽  
A D Redd ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Western Blot ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Western Blot Assay ◽  
The Usa ◽  
Simplex Virus

scholarly journals Limits in Reliability of Glycoprotein G-Based Type-Specific Serologic Assays for Herpes Simplex Virus Types 1 and 2

1999 ◽  
Vol 37 (2) ◽  
pp. 376-379 ◽  
Author(s):  
D. Scott Schmid ◽  
Denise R. Brown ◽  
Rosane Nisenbaum ◽  
Rae Lyn Burke ◽  
D’Anna Alexander ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Western Blot ◽  
Validation Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Western Blot Assay ◽  
Cross Sectional ◽  
Glycoprotein G ◽  
Simplex Virus ◽  
Hsv 1

Type-specific serologic assays for herpes simplex virus (HSV) types 1 and 2 based on glycoprotein G-1 (gG-1) (HSV-1) and gG-2 (HSV-2) discriminate between antibodies against HSV-1 and HSV-2. We previously developed a Western blot assay using gG-1 and gG-2 expressed in baculovirus, performed extensive validation studies, and determined that it was both sensitive and specific for type-specific detection of HSV antibody. Here we report that, among a cohort of Thai military recruits, the serostatus of some individuals changed from positive to negative over time (6.6% among those ever positive for HSV-1, and 14.9% among those ever positive for HSV-2). We tested a subset of these specimens in three other gG-based assays: an enzyme-linked immunosorbent assay, an immunoblot strip assay, and a Western blot assay. Positive-to-negative shifts occurred in every assay; the frequency of the shifts ranged from 6.1% to 21.2% of the specimen sets tested. There was only limited agreement among the assays concerning which individuals lost reactivity. This inaccuracy, exhibited by all of the assay protocols, was not predicted by validation studies employing specimens from cross-sectional studies and was most pronounced in HSV-2 testing. This argues for the inclusion of serial blood specimens in serologic assay validation procedures.

scholarly journals Evaluation of Three Glycoprotein G2-Based Enzyme Immunoassays for Detection of Antibodies to Herpes Simplex Virus Type 2 in Human Sera

1999 ◽  
Vol 37 (5) ◽  
pp. 1242-1246 ◽  
Author(s):  
Anna Maria Eis-Hübinger ◽  
Martin Däumer ◽  
Bertfried Matz ◽  
Karl Eduard Schneweis
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Enzyme Immunoassays ◽  
Glycoprotein G ◽  
Positive Reactivity ◽  
Human Sera ◽  
Specific Igg ◽  
Simplex Virus

Three new glycoprotein G-based enzyme immunoassays (ETI-HSVK-G 2, Sorin Diagnostics Biomedica [assay A]; HSV Type 2 Specific IgG ELISA, Gull Laboratories, Inc. [assay B]; Cobas Core HSV-2 IgG EIA, Roche [assay C]) for the detection of herpes simplex virus (HSV) type 2 (HSV-2)-specific antibodies were evaluated. By testing sera from 25 individuals with culture-proven HSV-2 infection, the assays showed a sensitivity of 96%. The specificities, evaluated with sera from 70 HSV antibody-negative children, 75 HSV antibody-positive children, and 69 HSV antibody-negative adults, were 100% for assay A, 96.2% for assay B, and 97.8% for assay C, respectively. Discrepant results by any of the three assays, i.e., reactivity of a specimen in only one or two assays, occurred with similar frequencies for HSV-seronegative individuals as well as HSV-seropositive children and adults. For sera with discrepant results, the positive reactivity was mostly low. Thus, for determination of the prevalence of HSV-2 antibodies, only concordantly positive results were considered. On the basis of the results obtained with sera from 41 adults with culture-proven HSV-1 infection and from 173 HSV-antibody-positive pregnant women, the HSV-2 seroprevalence was 9.8%. The results show that the new glycoprotein G2-based enzyme immunoassays are useful tools for the detection of type-specific HSV-2 antibodies. However, if only one assay is performed, careful interpretation of the results is indicated, especially if the exhibited reactivity is low, and for determination of the definitive HSV-2 serostatus, confirmatory assays may still be necessary.

scholarly journals Cervicovaginal Neutralizing Antibodies to Herpes Simplex Virus (HSV) in Women Seropositive for HSV Types 1 and 2

2003 ◽  
Vol 10 (3) ◽  
pp. 388-393 ◽  
Author(s):  
Francois-Xavier Mbopi-Kéou ◽  
Laurent Bélec ◽  
Julie Dalessio ◽  
Jérôme Legoff ◽  
Gérard Grésenguet ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Neutralizing Antibodies ◽  
Female Genital Tract ◽  
African Women ◽  
Specific Antibodies ◽  
Neutralizing Activity ◽  
Simplex Virus ◽  
Hiv Negative ◽  
Hsv 1

ABSTRACT Antibodies to herpes simplex virus type 1 (HSV-1) and HSV-2 of the immunoglobulin G (IgG) and IgA isotypes were detected in the cervicovaginal secretions (CVS) of 77 HSV-1- and HSV-2-seropositive but clinically asymptomatic African women by type-specific enhanced chemiluminescence Western blotting (ECL-WB). Of the 77 subjects, 34 were HIV negative, shedding HSV-2 DNA in their genital secretions; 20 were HIV positive, shedding HSV-2 DNA; and 23 were HIV negative, not shedding HSV-2 DNA. HSV-specific IgG was detected in CVS of nearly 70% of the women studied. HSV-specific IgA was found in CVS of 50% of the women studied. The distribution of CVS HSV-specific antibodies to each HSV type was highly heterogeneous, with a slight predominance of detectable IgG to HSV-1 (59%) over IgG to HSV-2 (41%), whereas the frequency of detectable IgA to HSV-1 (39%) was similar to that of IgA to HSV-2 (36%). The presence of detectable HSV-specific antibodies was inversely associated with HSV-2 DNA genital asymptomatic shedding but was not affected by HIV seropositivity. In addition, 13 of 77 (17%) CVS samples showed neutralizing activity against HSV-2, as assessed by an HSV-2 in vitro infectivity reduction assay. Neutralizing activity in CVS was associated with the presence of IgG and/or IgA antibodies to HSV-1 and/or to HSV-2 by ECL-WB. Among women whose CVS showed HSV-2-neutralizing activity, the specific activity of HSV-specific neutralizing antibodies was substantially (fivefold) higher in HSV-2 DNA shedders than in nonshedders. In conclusion, HSV-specific antibodies are frequently detected in CVS of asymptomatic African women seropositive for HSV-1 and HSV-2. A subset of these women had functional neutralizing activity against HSV-2 in their CVS. The origin of these antibodies and their role in HSV-2 disease of the female genital tract remain to be determined.

Epidemiology of herpes simplex virus in children by detection of specific antibodies in saliva

2001 ◽  
Vol 20 (3) ◽  
pp. 265-272 ◽  
Author(s):  
VIRGINIE MASSEREY SPICHER ◽  
PAUL BOUVIER ◽  
SUSANNA E. SCHLEGEL-HAUETER ◽  
ALFREDO MORABIA ◽  
CLAIRE-ANNE SIEGRIST
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Specific Antibodies ◽  
Simplex Virus

scholarly journals Genetic and Environmental Determination of Herpes Simplex Virus Type 1 Penetration into Non-permissive Rat XC Cells

1987 ◽  
Vol 68 (9) ◽  
pp. 2455-2459 ◽  
Author(s):  
I. Machuca ◽  
A. L. Epstein ◽  
Y. Michael ◽  
B. Jacquemont
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Simplex Virus
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