scholarly journals Studies on Multiplex RT-PCR for Detection of Avian Influenza Virus Type A Group and Specific H5 and H7 Subtypes

2012 ◽  
Vol 1 (1) ◽  
Author(s):  
Huaguang Lu
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Virus Type ◽  
Type A ◽  
Rt Pcr

scholarly journals Surveillance of avian influenza virus type A in semi-scavenging ducks in Bangladesh

2013 ◽  
Vol 9 (1) ◽  
pp. 196 ◽  
Author(s):  
Amina Khatun ◽  
Mohammed Giasuddin ◽  
Kazi Islam ◽  
Sazeda Khanom ◽  
Mohammed Samad ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Virus Type ◽  
Type A

Rapid identification of H5 avian influenza virus in chicken throat swab specimens using microfluidic real-time RT-PCR

2014 ◽  
Vol 6 (8) ◽  
pp. 2628 ◽  
Author(s):  
Ling Zhu ◽  
Cancan Zhu ◽  
Guoqing Deng ◽  
Long Zhang ◽  
Shumi Zhao ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Real Time ◽  
Throat Swab ◽  
Rapid Identification ◽  
Rt Pcr

scholarly journals Avian influenza and Newcastle disease virusindead chickens in markets in Dhaka, Bangladesh in 2011-2012

2017 ◽  
Vol 33 (1) ◽  
pp. 8-15
Author(s):  
LR Barman ◽  
RD Sarker ◽  
BC Das ◽  
EH Chowdhury ◽  
PM Das ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Newcastle Disease ◽  
Rapid Test ◽  
Type A ◽  
Antigen Test ◽  
Rt Pcr ◽  
Test Kit ◽  
Live Bird

A virological survey for avian influenza (AI) and Newcastle disease (ND) was conducted in two selected live bird markets (LBMs), namely Kaptan Bazar and Karwan Bazar in Dhaka city, Bangladesh from August 2011 to July 2012. A total of 513 dead chickens were collected. An immune-chromatographic rapid antigen test for Type A influenza virus and both conventional and real time RT-PCR were used for the detection and characterization of AI and ND viruses. All carcasses were first screened by the rapid antigen test kit and 93 were positive for Type A influenza virus. RT-PCR on a representative number of rapid antigen test positive samples (n = 24) confirmed the presence of Type A influenza virus and mostly H5 influenza virus (22 out of 24 tested samples). Influenza rapid test negative samples (n = 420) were subjected to routine necropsy. Heat stress, suffocation and physical injury were the most common cause of mortality (163 cases), followed by ND, suspected to be the cause of 85 deaths. On molecular investigation of these 85 samples, the presence of ND virus was confirmed in 59 and AI virus in 6; 15 were negative for both ND and AI viruses and 5 were unsuitable for investigation. Among the 59 ND confirmed cases 18 also contained AI virus. In summary, out of 513 carcasses 117 (22.81%) contained AI virus and 59 (11.50%) contained ND virus. Eighteen (3.51%) carcasses contained both AI and ND viruses. The findings suggest that both AI and ND should be considered as major threats to the poultry industry.Bangl. vet. 2016. Vol. 33, No. 1, 8-15

Development of a TaqMan MGB RT-PCR for the rapid detection of H3 subtype avian influenza virus circulating in China

2015 ◽  
Vol 217 ◽  
pp. 64-69 ◽  
Author(s):  
Qiaoyang Teng ◽  
Weixia Shen ◽  
Dawei Yan ◽  
Liping Yan ◽  
Xuesong Li ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Rapid Detection ◽  
Rt Pcr ◽  
H3 Subtype

A FULLY AUTOMATED PROCEDURE FOR THE HIGH THROUGHPUT DETECTION OF AVIAN INFLUENZA VIRUS BY REAL-TIME RT-PCR

2007 ◽  
Vol 2 (s1) ◽  
pp. e13-e13
Author(s):  
Montserrat Agüero ◽  
Elena San Miguel ◽  
Azucena Sánchez ◽  
Concepción Gómez-Tejedor ◽  
Miguel Angel Jiménez-Clavero
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Real Time ◽  
High Throughput ◽  
Rt Pcr ◽  
High Throughput Detection

scholarly journals Prevalence of Newcastle Disease Virus and Avian Influenza Virus (H7N3) in Poultry at Karachi

2020 ◽  
Vol 11 (1) ◽  
pp. 1-6
Author(s):  
Amjad Ali Channa ◽  
Nazeer Hussain Kalhoro ◽  
Zaheer Ahmed Nizamani ◽  
Ayaz Hussain Mangi ◽  
Jamila Soomro
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Avian Influenza Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Virus Isolation ◽  
Economic Losses ◽  
Rt Pcr ◽  
Agar Gel

Background: Poultry is largest and rapidly growing sector of livestock in Pakistan. It is mainly influenced by viral pathogens such as Newcastle Disease Virus (NDV) and Avian Influenza Virus (H7N3). These viruses cause severe disease in poultry and leads to heavy economic losses throughout the world. The outbreaks of these pathogens have been increased in last few decades. Therefore, the study about antigenic prevalence is needed to know about the emergence of these pathogenic viruses, and to get rid of severe ailments associated with reduced poultry production. Objectives: To determine the prevalence of Newcastle Disease Virus (NDV), Avian Influenza Virus (H7N3) and co-infections in poultry flocks at Karachi. Methodology: For detection of NDV and H7N3, a total of 200 tracheal swabs were collected and tested through virus isolation (V.I); the sample with positive virus isolation were tested through agar gel precipitation (AGP) and then the RNA was isolated through TRI Reagent, which was further tested through reverse transcription polymerase chain reaction (RT-PCR). Results: The virus isolation showed that 58% of samples were positive for various viruses. Agar gel precipitation (AGP) revealed that the occurrence of NDV, H7N3 and ND+H7 were 50%, 8% and 38%, respectively. RT-PCR for F and HA gene of NDV and H7N3 confirmed the presence of NDV and H7N3 in the poultry. Conclusion: It is concluded that NDV and H7N3 are circulating in the flocks causing co-infections, therefore it is important to know the field challenge of viruses and to prepare vaccine of circulating serotype of virus to mitigate the rate of infection.

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