Surveillance of avian influenza virus type A in semi-scavenging ducks in Bangladesh

Abstract Background Information regarding comparison of the environmental prevalence of avian influenza virus (AIVs), before and after massive poultry vaccinations, is limited. Our study aimed to detect differences in the prevalence of AIVs type A and subtypes H5, H7, and H9 before and after the September 2017 massive poultry vaccination, across different sampling places and types. Methods We collected 55 130 environmental samples from 11 cities in Zhejiang Province (China) between March 2013 and December 2018. Multivariate logistic regression analyses were conducted to determine the prevalence of AIV type A and subtypes H5, H7, and H9 across different sampling places and types, before and after massive poultry vaccination. Results After the vaccination, contamination risk of AIV type A (adjusted odds ratio [aOR] = 1.08; 95% confidence interval [CI], 1.03–1.14) and subtype H9 (aOR = 1.58; 95% CI, 1.48–1.68) increased, and that of subtype H7 (aOR = 0.12; 95% CI, 0.10–0.14) decreased. Statistically significant decreased risk for H7 subtype contamination and increased risk for H9 subtype contamination were observed in backyard poultry flocks, live poultry markets, and slaughtering/processing plants. Swabs from poultry cages and slaughtering tables showed a statistically significant increased risk for H5 subtype contamination. The prevalence of H7 subtype decreased statistically significantly, whereas that of H9 subtype increased across the 5 sample types (poultry cages swabs, slaughtering table swabs, poultry feces, poultry drinking water, and poultry sewage). Conclusions Despite the sharp decrease in H7 subtype prevalence, reduction measures for AIV circulation are still imperative, given the high type A prevalence and the increase in H9 subtype contamination across different sampling places and types.

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Seroepizootiological investigations of animals from Obedska bara locality for presence of Avian influenza virus

Veterinarski glasnik ◽

10.2298/vetgl1006307d ◽

2010 ◽

Vol 64 (5-6) ◽

pp. 307-317

Author(s):

Bosiljka Djuricic ◽

Ana Samokovlija ◽

Zivka Ilic ◽

Dragan Bacic ◽

Sonja Radojicic ◽

...

Keyword(s):

Influenza Virus ◽

Hong Kong ◽

Avian Influenza ◽

Avian Influenza Virus ◽

Influenza A ◽

Migratory Birds ◽

Type A ◽

Influenza Viruses ◽

High Rate ◽

Virus Serotype

The disease caused by Influenza viruses has been well known for a very long time. In the recent period there has been noted an occurrence of pandemics caused by Influenza viruses type A with a high rate of mortality. The ongoing pandemic caused by avian influenza virus serotype H9N9 began in Hong Kong in 1992, and another pandemic caused by serotype H5N1 began in China (Hong Kong) in 1999. The world wide spreading of these viruses occurred due to migratory birds. Avian influenza was confirmed in Serbia in 2007. The goal of this study was to examine whether the avian influenza viruses type A circulate in the region of the Obedska bara marsh, which is a famous resort for many birds in Serbia, as well as many birds migrating from Europe to Africa and vice versa. The samples of blood sera of many animal species (123 samples from fowl, 64 samples from donkeys, 40 samples from horses) were tested by serologic reaction of inhibition of haemmaglutination (IHA) for the presence of antibodies to influenza A subtypes H5N1, H5N2, H5N3, H7N1 and H7N2. Also, the samples of blood sera of experimental chicken exposed to wild life in Obedska bara (sentinel species) were tested. Antibodies to subtypes H5N1, H5N2, H5N3, H7N1 and H7N2 were found in chicken from Dec, Boljevci, Petrovcic and Kupinovo villages but no antibodies were found in blood sera from hams from Dobanovci, Jakovo, Becmen and Surcin villages. From 23 samples from ducks antibodies were detected in 3 samples, and from 22 geese blood sera antibodies were found in 4 samples. From a total of 40 horse blood sera tested one was tested positive, and from 64 donkey sera 17 were positive for the presence of antibodies for avian influenza type A. In blood sera of experimental chicken antibodies were found by subtype H5N1 with corrections with H5N2 and H7N1.

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Seroprevalence and Detection of Avian Influenza Type A in Ducks at Nikli and Bajitpur Upazila of Bangladesh

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v13i1.23750 ◽

2015 ◽

Vol 13 (1) ◽

pp. 11-17 ◽

Cited By ~ 1

Author(s):

MZ Hassan ◽

BC Das ◽

MS Mahmud ◽

MA Amin ◽

MA Yousuf ◽

...

Keyword(s):

Influenza Virus ◽

Avian Influenza ◽

Avian Influenza Virus ◽

Rna Extraction ◽

Age Groups ◽

Type A ◽

Influenza Viruses ◽

M Gene ◽

Long Distance ◽

Influenza Type

Waterfowl are the natural reservoir of avian influenza viruses and ducks may play a role in the maintenance of avian influenza type A. The aim of the present study was to investigate the seroprevalence and detection of avian influenza virus (AIV) type A in duck. This study was carried out during July 2013 to December 2013 on AIV type A from semi-scavenging farm at Nikli and Bajitpur upazila of Kishoregonj district in Bangladesh. A total of 368 blood samples were collected from duck and tested by indirect ELISA for seroprevalence. For detection of AIV type A, The cloacal swabs were collected from 75 duck and subjected to RNA extraction and real time RT-PCR (rRT-PCR) with specific primer and probe for detection of matrix (M) gene. The average seroprevalance of AIV type A in seven different age groups was found to be 90.21%. The highest (25.81 %) seroprevalence was found in 5 months age of birds and the lowest (2.44 %) was found in 12 months age of birds. As regard to area distribution, the average degree of seroprevalence was 93.51% from Nikli had the highest order than Bajitpur (86.88%) upazila of Bangladesh. In case of cloacal sample by using rRTPCR, out of 15 pooling cloacal samples, two pooling samples (13.33%) that contain 10 samples were positive and 13 pooling samples showed negative (86.67%) for AIV type A in duck. It can be concluded that the long distance movement of duck flocks, may influence outbreak of avian influenza virus (AIV) type A among different poultry species in Bangladesh. Therefore, it needs to develop control strategy for future dissemination of AIV in duck population.DOI: http://dx.doi.org/10.3329/bjvm.v13i1.23705Bangl. J. Vet. Med. (2015). 13 (1): 5-9

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Detection of Specific type-A Antigen and Specific Antibody against Avian Influenza virus in Native Duck at Netrokona district of Bangladesh

Microbes and Health ◽

10.3329/mh.v4i2.30561 ◽

2016 ◽

Vol 4 (2) ◽

pp. 5-10

Author(s):

MM Mafizul Islam ◽

Mir Rowshan Akter ◽

Md Mostafizer Rahman ◽

Md Atiqul Haque ◽

Md Karim Uddin ◽

...

Keyword(s):

Influenza Virus ◽

Avian Influenza ◽

Positive Result ◽

Avian Influenza Virus ◽

Antibody Titer ◽

Specific Antibody ◽

Type A ◽

Aged Group ◽

Influenza Type ◽

Test Kit

The present study was conducted on unvaccinated native ducks of different age groups to determine specific antibody titer level against Avian Influenza virus (AIV) by indirect Enzyme Linked Immunosorbent Assay (iELISA) and to detect avian influenza type A virus antigen by rapid AIV antigen test kit at Netrokona district of Bangladesh. This study showed that AIV specific antibody positive cases were 78 out of 90 blood serum samples and the highest antibody titer was 2323 and lowest antibody titer was 256. The total 86.67% sera samples were showed positive result. The study showed that 66.66% sera sample were positive against AIV at 3-4 month of aged group and the highest, lowest and mean antibody titer were 1428, 256 and 906.3 respectively. On the other hand 78% sera sample were positive against AIV at 5-6 month aged group and the highest, lowest and mean antibody titer were 1675 , 451 and 1083.6 respectively. The sera sample collected from 7-8 month aged group showed 88.9% positive and the highest, lowest and mean antibody titer were 1857, 578 and 1285.5 respectively. The sera sample collected from 9-10 month of aged group showed 100% positive against AIV and the highest, lowest and mean antibody titer were 197l, 638 and 1571.5 respectively .The sera sample collected from duck of ?11 month aged group were 100% positive against AIV and the highest, lowest and mean antibody titer were 2323, 1423 and 1813.7 respectively. Tracheal and cloacal swabs from ducks with antibody titer more than 1813.778 were tested for the avian influenza type A antigen by Anigen Rapid AIV Ag test kit. The above sample showed 20% positive result. In conclusion it is evident that Avian influenza virus-specific antibody was successfully detected through commercially available Avian influenza virus antibody test kit (ELISA Kit) and the virus induced a significant antibody titer indicating the affecting virus was absolutely AIV.

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Epidemiology of duck as reservoir of Avian Influenza Virus in Bangladesh

Asian Journal of Medical and Biological Research ◽

10.3329/ajmbr.v4i1.36816 ◽

2018 ◽

Vol 4 (1) ◽

pp. 14-20

Author(s):

Md Zakir Hassan ◽

Md Mamunur Rahman ◽

Bidhan Chandra Das ◽

Md Al Amin ◽

Salma Sultana ◽

...

Keyword(s):

Influenza Virus ◽

Avian Influenza ◽

Blood Serum ◽

Avian Influenza Virus ◽

Real Time ◽

Serum Sample ◽

Prevalence Rate ◽

Type A ◽

Blood Serum Sample ◽

Influenza Type

This research work was conducted to detect the prevalence and incidence of Avian Influenza Virus (AIV) in Duck at hoar area of Kishorganj district in Bangladesh. The study period was July 2013 to December 2013 and the molecular work was done in Central Disease Investigation Laboratory (CDIL), Dhaka. A total number of 736 blood serum sample and 150 cloacal swab sample were collected from asymptomatic semi scavenging duck above 4 month of age. Blood serum sample was tested to detect the prevalence of Avian Influenza Virus A (AIV) specific antibody through indirect Enzyme Linked Immunosorbent Essay (ELISA) and cloacal sample was tested to detect the incidence of AIV through real time Polymerase Chain reaction (PCR). A total number of 736 blood serum sample were tested in which 684 is positive (+ve) of Avian Influenza type A antibody and 52 are negative (-ve) of Avian Influenza type A. The prevalence rate was 92.93%. The tested result shown that prevalence rate was in 4-6 month of age as 93.96%, as 7-9 month of age as 92.92 % and in 10-12 month of age as 91.91%. A total number of 15 pooling sample from 75 cloacal samples was conducted for detection of AIV that shed in environment. After calculating the result through real time Reverse Transcription (RT) - PCR, it was shown that 2 pooling sample was positive (13.33%) for AIV and 13 pooling sample was negative (86.87%) for AIV. So incidence rate was 13.33% for AIV. This duck can transmit the AIV in the surrounding poultry population and clinical outbreaks may occur. After analysis of ELISA & PCR result it was shown that duck act as a natural reservoir of AIV in Bangladesh.Asian J. Med. Biol. Res. March 2018, 4(1): 14-20

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New avian influenza virus A H5N7 identified in ducks in Denmark

Weekly releases (1997–2007) ◽

10.2807/esw.07.39.02300-en ◽

2003 ◽

Vol 7 (39) ◽

Author(s):

◽

Keyword(s):

Influenza Virus ◽

Avian Influenza ◽

Avian Influenza Virus ◽

Influenza A Virus ◽

Virus Type ◽

Influenza A ◽

Influenza Virus A ◽

First Time

A new influenza A virus type, H5N7, has been identified for the first time in ducks in Denmark

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A biosensor based on electroactive dipyrromethene-Cu(II) layer deposited onto gold electrodes for the detection of antibodies against avian influenza virus type H5N1 in hen sera

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-015-8949-y ◽

2015 ◽

Vol 407 (25) ◽

pp. 7807-7814 ◽

Cited By ~ 8

Author(s):

Urszula Jarocka ◽

Róża Sawicka ◽

Anna Stachyra ◽

Anna Góra-Sochacka ◽

Agnieszka Sirko ◽

...

Keyword(s):

Influenza Virus ◽

Avian Influenza ◽

Avian Influenza Virus ◽

Virus Type ◽

Gold Electrodes

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Highly pathogenic avian influenza reported in Russian bird populations

Weekly releases (1997–2007) ◽

10.2807/esw.10.32.02771-en ◽

2005 ◽

Vol 10 (32) ◽

Author(s):

Collective Editorial team

Keyword(s):

Influenza Virus ◽

Avian Influenza ◽

Avian Influenza Virus ◽

Virus Type ◽

Highly Pathogenic Avian Influenza ◽

Infected Bird ◽

Highly Pathogenic ◽

Bird Populations ◽

Pathogenic Avian Influenza ◽

Pathogenic Avian Influenza Virus

The highly pathogenic avian influenza virus type A(H5N1), which continues to infect humans in Vietnam and Indonesia, has been reported to have infected bird populations in Russia

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