Introduction:
Novel Therapeutic Phytochemical studies are based on the use of plants in the production of
drugs. This present study was carried out to the screening of phytochemicals and quantitative analysis, to assess In-vitro
Antioxidant activities of the hydroethanolic leaf extract of Caesalpinia pulcherrima. FT-IR and GC-MS analysis of
hydroethanolic leaf extract of Caesalpinia pulcherrima.
Methods:
The investigation of qualitative and determination of quantitative phytochemical analysis were performed using
standard procedures. The total alkaloid, flavonoid, phenols, and tannin content were determined spectrophotometrically.
DPPH (2,2-Diphenyl-1-picrylhydrazyl) free radical scavenging activity, ABTS (2,2’-azino-bis (3-ethylbenzothiazoline-6-
sulfonic acid) free radical scavenging activity of hydroethanolic leaf extract of Caesalpinia pulcherrima was estimated by
standard protocol. The identification of functional groups using plant leaf extracts by FTIR and GCMS.
Results:
The hydroethanolic leaf extract of Caesalpinia pulcherrima gives positive results for alkaloids, flavonoids,
phenols, carbohydrates, tannins, amino acids, and proteins. The content of total alkaloids, flavonoids, phenols, and tannins
varies from 8.4 AE/g, 3.3 CE/g, 2.9, and 3.2 GAE/g respectively. The antioxidant assay and IC50 values were found to be
DPPH (43.3µg/ml), ABTS (46.1µg/ml), and ascorbic acid (41µg/ml), respectively and concentration ranging from 20 to
100µgml. Functional groups were also identified using the extract by FTIR and GCMS.
Conclusions:
From this work, it can be concluded that the presence of Phyto-component makes the plant useful in the
treatment of various diseases. As such, it has been found that the hydroethanolic leaf extract of the plant contains more
components and is beneficial for further research.
Phytochemical Analysis, In Vitro Evaluation of Antioxidant and Free Radical Scavenging Activity of Simarouba glauca Seeds
