scholarly journals The Influence of Cu and Co Nanoparticles on Growth Characteristics and Biochemical Structure of Mentha Longifolia in Vitro

2016 ◽  
Vol 4 (2) ◽  
pp. 31-39 ◽  
Author(s):  
T.E. Talankova-Sereda ◽  
K.V. Liapina ◽  
E. A. Shkopinskij ◽  
A.I. Ustinov ◽  
A.V. Kovalyova ◽  
...  
Keyword(s):  
Growth Characteristics ◽  
Mentha Longifolia ◽  
Co Nanoparticles

The Influence of Cu и Co Nanoparticles on Growth Characteristics and Biochemical Structure of Mentha Longifolia In Vitro

2016 ◽  
pp. 427-436 ◽  
Author(s):  
T. E. Talankova-Sereda ◽  
K. V. Liapina ◽  
E. A. Shkopinskij ◽  
A. I. Ustinov ◽  
A. V. Kovalyova ◽  
...  
Keyword(s):  
Growth Characteristics ◽  
Mentha Longifolia ◽  
Co Nanoparticles

scholarly journals Growth characteristics of human bone marrow mesenchymal stromal cells at cultivation on synthetic polyelectrolyte nanofilms in vitro

Heliyon ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. e06517
Author(s):  
Lyudmila M. Mezhevikina ◽  
Dmitriy A. Reshetnikov ◽  
Maria G. Fomkina ◽  
Nurbol O. Appazov ◽  
Saltanat Zh. Ibadullayeva ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Growth Characteristics ◽  
Human Bone ◽  
Human Bone Marrow

scholarly journals In vitro growth characteristics of five candidate aquaculture probiotics and two fish pathogens grown in fish intestinal mucus

2004 ◽  
Vol 231 (1) ◽  
pp. 145-152 ◽  
Author(s):  
Niall G Vine ◽  
Winston D Leukes ◽  
Horst Kaiser
Keyword(s):  
Growth Characteristics ◽  
In Vitro Growth ◽  
Fish Pathogens ◽  
Intestinal Mucus

scholarly journals Low Oxygen Tension Potentiates Proliferation and Stemness but Not Multilineage Differentiation of Caprine Male Germline Stem Cells

2021 ◽  
Author(s):  
Shiva Pratap Singh ◽  
Suresh Dinkar Kharche ◽  
Manisha Pathak ◽  
Ravi Ranjan ◽  
Yogesh Kumar Soni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
Growth Characteristics ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Germline Stem Cells ◽  
Multilineage Differentiation ◽  
Low Oxygen ◽  
Differentiation Potential

Abstract The milieu of testicular germline stem cells (mGSCs) is characterized as low oxygen (O2) environment, whereas, there in-vitro expansion is typically performed under normoxia (20-21% O2). Here, we evaluated and compared the culture and multilineage differentiation characteristics of enriched (through differential platting and percoll density centrifugation) caprine mGSCs (cmGSCs) under hypoxic (5% O2) and normoxic (21% O2) culture conditions. For this, in addition to growth characteristics and population-doubling time (PDT); viability, proliferation, senescence, and expression of key-markers of adhesion (β-integrin and E-Cadherin) and stemness (OCT-4, THY-1 and UCHL-1) were evaluated and compared under normoxia and hypoxia. Moreover, the extent of multilineage differentiation (neurogenic, adipogenic, and chondrogenic differentiation) was assessed. The survival, viability and proliferation were significantly promoted and PDT was reduced (p < 0.05), thus yielding a higher number of viable cells with larger colonies under hypoxia. Furthermore, expression of stemness and adhesion markers was distinctly increased under lowered O2 condition. Conversely, the presence of differentiated regions and expression of differentiation specific key genes [C/EBPα (adipogenic), nestin and β-tubulin (neurogenic), and COL2A1 (chondrogenic)] were significantly (p < 0.05) reduced under hypoxic conditions. These data demonstrate that culturing cmGSCs under hypoxia augments the growth characteristics, and stemness but not the multilineage differentiation potential of cmGSCs as compared with normoxia. These data are important for the development of robust methodologies for ex-vivo expansion and lineage-committed differentiation of cmGSCs for clinical applications.

scholarly journals Growth characteristics of the novel goose parvovirus SD15 strain in vitro

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Jinyue Zhang ◽  
Peng Liu ◽  
Yuanyuan Wu ◽  
Mingshu Wang ◽  
Renyong Jia ◽  
...  
Keyword(s):  
Growth Characteristics ◽  
The Novel ◽  
Goose Parvovirus

scholarly journals Effect of explant source, perlite nanoparticles and TiO2/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
R. Ebadollahi ◽  
S. Jafarirad ◽  
M. Kosari-Nasab ◽  
S. Mahjouri
Keyword(s):  
Volatile Compounds ◽  
Culture Medium ◽  
Hypericum Perforatum ◽  
Callus Cultures ◽  
Growth Characteristics ◽  
Phytochemical Composition ◽  
Adverse Impacts ◽  
Explant Source ◽  
Metabolite Profiles

Abstract It appears that the biologically-synthesized nanoparticles (NPs) have potential to perform as effective elicitors for the production of valuable secondary metabolites in plants. Besides, it has been reported that the toxicity of the biologically-synthesized NP is not as much as that of the chemically-synthesized NPs. Therefore, it is necessary to test their advantages aspects. In this study, the physical synthesis of perlite NPs and biologically-synthesis of TiO2/perlite nanocomposites (NCs) were conducted. Subsequently, their effects and explant source influence on the growth characteristics and secondary metabolite profiles of Hypericum perforatum callus cultures were evaluated. According to the obtained results, morphology of the synthesized perlite NPs and TiO2/perlite NCs were mesoporous and spherical with sizes ranging about 14.51–23.34 and 15.50–24.61 nm, respectively. Addition of perlite NPs and TiO2/perlite NCs to the culture medium at the concentration range of 25–200 mg/L showed no adverse impacts on the growth characteristics of H. perforatum calli. According to the GC-MS analysis, the stress caused by perlite NPs and TiO2/perlite NCs led to an increase in the variety, amount and number of volatile compounds. The calli obtained from in vitro grown plants produced more volatile compounds relative to the calli obtained from field grown plants under the nanomaterial stress conditions. The production of hypericin and pseudohypericin were also determined in the callus cultures under desired nanomaterials elicitation. Accordingly, our results suggest that perlite NPs and TiO2/perlite NCs can possibly be considered as effective elicitors for the production of volatile compounds, hypericin, and pseudohypericin in callus cultures of H. perforatum.

RENAL ALLOGRAFT-INFIL TRATING LYMPHOCYTES A PROSPECTIVE ANALYSIS OF IN VITRO GROWTH CHARACTERISTICS AND CLINICAL RELEVANCE

1992 ◽  
Vol 53 (2) ◽  
pp. 329-337 ◽  
Author(s):  
ALLAN D. KIRK ◽  
MAHA A. IBRAHIM ◽  
R. RANDAL BOLLINGER ◽  
DEBORAH V. DAWSON ◽  
OLIVERA J. FINN
Keyword(s):  
Clinical Relevance ◽  
Renal Allograft ◽  
Growth Characteristics ◽  
Prospective Analysis ◽  
In Vitro Growth
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