Characterization of Serum Tartrate-Resistant Acid Phosphatase and Development of a Direct Two-Site Immunoassay

Abstract A tartrate-resistant acid phosphatase was isolated from a human leukemic spleen by freeze-thawing in saline and purified by repeated chromatography on carboxymethyl-cellulose. The purified enzyme has a molecular weight of 64 000. It catalyzes the hydrolysis of inorganic and organic pyrophosphate as well as the phenolic ester of monoorthophosphate, with optimal activity between pH 5 and 6. However, there is no activity toward mono-orthophosphate esters of aliphatic alcohols. The present data have identified its catalytic function as a pyrophosphatase. However, it has properties different from the pyrophosphatase previously observed in normal animal tissues.

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Characterization of mineral deposits formed in cultures of a hamster tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) double-positive cell line (CCP)

Cell and Tissue Research ◽

10.1007/s00441-002-0587-y ◽

2002 ◽

Vol 309 (2) ◽

pp. 269-279 ◽

Cited By ~ 3

Author(s):

Hisako Sakiyama ◽

Takashi Nonaka ◽

Yoshinori Kuboki ◽

Yoshio Hirabayasi ◽

Kazuko Yoshida ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Cell Line ◽

Mineral Deposits ◽

Tartrate Resistant Acid Phosphatase ◽

Double Positive ◽

Double Positive Cell

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Characterization of the mouse tartrate-resistant acid phosphatase (trap) gene promoter

Journal of Bone and Mineral Research ◽

10.1002/jbmr.5650100413 ◽

2009 ◽

Vol 10 (4) ◽

pp. 601-606 ◽

Cited By ~ 58

Author(s):

S.V. Reddy ◽

J.E. Hundley ◽

J.J. Windle ◽

O. Alcantara ◽

R. Linn ◽

...

Keyword(s):

Acid Phosphatase ◽

Gene Promoter ◽

Tartrate Resistant Acid Phosphatase ◽

Trap Gene

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Development and Characterization of Novel Monoclonal Antibodies Against Tartrate-Resistant Acid Phosphatase 5

Hybridoma ◽

10.1089/hyb.2006.25.358 ◽

2006 ◽

Vol 25 (6) ◽

pp. 358-366 ◽

Cited By ~ 1

Author(s):

Tatsuya Ohashi ◽

Toshihide Miura ◽

Yoshihiko Igarashi ◽

Iwao Kiyokawa ◽

Yasuhito Sato ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Acid Phosphatase ◽

Tartrate Resistant Acid Phosphatase

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Generation and characterization of monoclonal antibodies to human type-5 tartrate-resistant acid phosphatase: development of a specific immunoassay of the isoenzyme in serum

Clinical Chemistry ◽

10.1093/clinchem/41.10.1495 ◽

1995 ◽

Vol 41 (10) ◽

pp. 1495-1499 ◽

Cited By ~ 37

Author(s):

P Chamberlain ◽

J Compston ◽

T M Cox ◽

A R Hayman ◽

R C Imrie ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Acid Phosphatase ◽

Premenopausal Women ◽

Tartrate Resistant Acid Phosphatase ◽

Time Resolved ◽

Specific Serum ◽

Serum Trap ◽

Human Osteoclast ◽

The Mean

Abstract We have characterized four monoclonal antibodies (mAbs) to the purple ("tartrate-resistant," band 5) acid phosphatase of the human osteoclast (TRAP) and used these to develop a specific serum immunoassay. All four mAbs are of high affinity (Ka = 1-5 x 10(8) L/mol) with a very fast Kassoc (0.2-2.0 x 10(5) L mol-1 s-1) and a moderate Kdissoc (1-3 x 10(-3) s). Two of the mAbs were selected to develop a time-resolved fluorescence immunoassay to measure serum concentrations of TRAP. The mean serum immunoreactive TRAP in a group of healthy premenopausal women and men was 3.7 +/- 1.8 micrograms/L (mean +/- SD) and 3.5 +/- 1.6 micrograms/L, respectively. Significantly higher concentrations of TRAP were found in postmenopausal women (6.3 +/- 2.3 micrograms/L) and in eight patients with Gaucher disease (19.3 +/- 4.7 micrograms/L). Further studies are required to investigate the value of serum TRAP as a marker of bone resorption.

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