Isolation and phenotypic characterization of a multinucleated tartrate-resistant acid phosphatase–positive bone marrow macrophage

2011 ◽  
Vol 39 (3) ◽  
pp. 339-350.e3 ◽  
Author(s):  
Erik Karlström ◽  
Barbro Ek-Rylander ◽  
Mikael Wendel ◽  
Göran Andersson
Keyword(s):  
Bone Marrow ◽  
Acid Phosphatase ◽  
Phenotypic Characterization ◽  
Tartrate Resistant Acid Phosphatase ◽  
Bone Marrow Macrophage

Phenotypic Characterization of Distinct Human Bone Marrow-Derived MSC Subsets

2009 ◽  
Vol 1176 (1) ◽  
pp. 124-134 ◽  
Author(s):  
Hans-Jörg Bühring ◽  
Sabrina Treml ◽  
Flavianna Cerabona ◽  
Peter De Zwart ◽  
Lothar Kanz ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Phenotypic Characterization

Biochemical characterization of the tartrate-resistant acid phosphatase of human spleen with leukemic reticuloendotheliosis as a pyrophosphatase.

1977 ◽  
Vol 23 (1) ◽  
pp. 89-94 ◽  
Author(s):  
K W Lam ◽  
L T Yam
Keyword(s):  
Acid Phosphatase ◽  
Biochemical Characterization ◽  
Normal Animal ◽  
Tartrate Resistant Acid Phosphatase ◽  
Animal Tissues ◽  
Human Spleen ◽  
Optimal Activity ◽  
Catalytic Function ◽  
Hydrolysis Of

Abstract A tartrate-resistant acid phosphatase was isolated from a human leukemic spleen by freeze-thawing in saline and purified by repeated chromatography on carboxymethyl-cellulose. The purified enzyme has a molecular weight of 64 000. It catalyzes the hydrolysis of inorganic and organic pyrophosphate as well as the phenolic ester of monoorthophosphate, with optimal activity between pH 5 and 6. However, there is no activity toward mono-orthophosphate esters of aliphatic alcohols. The present data have identified its catalytic function as a pyrophosphatase. However, it has properties different from the pyrophosphatase previously observed in normal animal tissues.

Characterization of the mouse tartrate-resistant acid phosphatase (trap) gene promoter

2009 ◽  
Vol 10 (4) ◽  
pp. 601-606 ◽  
Author(s):  
S.V. Reddy ◽  
J.E. Hundley ◽  
J.J. Windle ◽  
O. Alcantara ◽  
R. Linn ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Gene Promoter ◽  
Tartrate Resistant Acid Phosphatase ◽  
Trap Gene

Development and Characterization of Novel Monoclonal Antibodies Against Tartrate-Resistant Acid Phosphatase 5

Hybridoma ◽  
2006 ◽  
Vol 25 (6) ◽  
pp. 358-366 ◽  
Author(s):  
Tatsuya Ohashi ◽  
Toshihide Miura ◽  
Yoshihiko Igarashi ◽  
Iwao Kiyokawa ◽  
Yasuhito Sato ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Acid Phosphatase ◽  
Tartrate Resistant Acid Phosphatase

Generation and characterization of monoclonal antibodies to human type-5 tartrate-resistant acid phosphatase: development of a specific immunoassay of the isoenzyme in serum

1995 ◽  
Vol 41 (10) ◽  
pp. 1495-1499 ◽  
Author(s):  
P Chamberlain ◽  
J Compston ◽  
T M Cox ◽  
A R Hayman ◽  
R C Imrie ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Acid Phosphatase ◽  
Premenopausal Women ◽  
Tartrate Resistant Acid Phosphatase ◽  
Time Resolved ◽  
Specific Serum ◽  
Serum Trap ◽  
Human Osteoclast ◽  
The Mean

Abstract We have characterized four monoclonal antibodies (mAbs) to the purple ("tartrate-resistant," band 5) acid phosphatase of the human osteoclast (TRAP) and used these to develop a specific serum immunoassay. All four mAbs are of high affinity (Ka = 1-5 x 10(8) L/mol) with a very fast Kassoc (0.2-2.0 x 10(5) L mol-1 s-1) and a moderate Kdissoc (1-3 x 10(-3) s). Two of the mAbs were selected to develop a time-resolved fluorescence immunoassay to measure serum concentrations of TRAP. The mean serum immunoreactive TRAP in a group of healthy premenopausal women and men was 3.7 +/- 1.8 micrograms/L (mean +/- SD) and 3.5 +/- 1.6 micrograms/L, respectively. Significantly higher concentrations of TRAP were found in postmenopausal women (6.3 +/- 2.3 micrograms/L) and in eight patients with Gaucher disease (19.3 +/- 4.7 micrograms/L). Further studies are required to investigate the value of serum TRAP as a marker of bone resorption.

scholarly journals Demonstration of tartrate-resistant acid phosphatase in un-decalcified, glycolmethacrylate-embedded mouse bone: a possible marker for (pre)osteoclast identification.

1986 ◽  
Vol 34 (10) ◽  
pp. 1317-1323 ◽  
Author(s):  
F P van de Wijngaert ◽  
E H Burger
Keyword(s):  
Bone Marrow ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Mononuclear Cells ◽  
Histochemical Demonstration ◽  
Splenic Tissue ◽  
Tartrate Resistant Acid Phosphatase ◽  
Excellent Preservation ◽  
Histochemical Marker

Fixed, undecalcified mouse long bones were embedded in glycol methacrylate (GMA), sectioned, and incubated for acid phosphatase in the presence or absence of tartrate, to investigate the feasibility of tartrate-resistant acid phosphatase as a histochemical marker for osteoclast identification. Naphthol AS-BI phosphate was used as the substrate and hexazonium pararosanaline as coupler. Cytocentrifuge preparations of mouse, rat, and quail bone marrow or frozen and GMA sections of mouse splenic tissue were used as controls to specify acid phosphatase activity. After adequate fixation, acid phosphatase activity sensitive to tartrate inhibition (TS-AP) was demonstrated in macrophages from spleen, bone marrow, and loose connective tissue surrounding bone rudiments. Acid phosphatase activity resistant to tartrate inhibition (TR-AP), was detected in multi-nuclear osteoclasts and in some mononuclear cells from bone marrow and periosteum. In cytocentrifuge preparations and frozen sections of mouse spleen, TR-AP was demonstrated after simultaneous incubation with substrate and tartrate. In GMA sections, however, TR-AP could only be demonstrated after pre-incubation with tartrate before application of substrate. We suggest that histochemical demonstration of TR-AP versus TS-AP on GMA-embedded bone sections by means of a pre-incubation method can be used as an identification marker of (pre)osteoclasts. Plastic embedding is recommended for its excellent preservation of morphology and enzyme activity.

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