Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy

Abstract Background Intracranial meningiomas display a variable amount of peritumoral brain edema (PTBE), which can significantly impact perioperative morbidity. The role of microcirculatory disturbances in the pathogenesis of PTBE is still debated. The aim of this study was to microscopically demonstrate and intraoperatively quantify, for the first time, the alterations to microcirculation in PTBE using sidestream dark-field (SDF) imaging. Methods Adult patients with WHO grade I meningiomas were recruited over a 9-month period and divided into 2 groups depending on the absence (NE group) or the presence (E group) of PTBE. In vivo intraoperative microcirculation imaging was performed in the peritumoral area before and after microsurgical resection. Results Six patients were included in the NE group and 6 in the E group. At the baseline in the NE group, there was a minor decrease in microcirculatory parameters compared to normal reference values, which was probably due to the mass effect. In contrast, microcirculatory parameters in the E group were significantly altered, affecting both vessel density and blood flow values, with a drop of approximately 50% of normal values. Surgical resection resulted in a quasi-normalization of microcirculation parameters in the NE group, whereas in the E group, even if all parameters statistically significantly improved, post-resection values remained considerably inferior to those of the normal reference pattern. Conclusion Our study confirmed significant alterations of microcirculatory parameters in PTBE in meningiomas. Further in vivo SDF imaging studies may explore the possible correlation between the severity of these microcirculatory alterations and the postoperative neurological outcome.

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Assessment of microcirculatory perfusion in healthy anesthetized cats undergoing ovariohysterectomy using sidestream dark field microscopy

Journal of Veterinary Emergency and Critical Care ◽

10.1111/vec.12296 ◽

2015 ◽

Vol 25 (3) ◽

pp. 349-357 ◽

Cited By ~ 1

Author(s):

Michelle E. Goodnight ◽

Edward S. Cooper ◽

Amy L. Butler

Keyword(s):

Dark Field ◽

Sidestream Dark Field ◽

Microcirculatory Perfusion ◽

Dark Field Microscopy

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The experience of using dark-field microscopy to assess damage to the endothelial glycocalyx in rheumatoid arthritis

HERALD of North-Western State Medical University named after I I Mechnikov ◽

10.17816/mechnikov43846 ◽

2021 ◽

Vol 12 (4) ◽

pp. 73-80

Author(s):

Timur D. Vlasov ◽

Daniel A. Shimanski ◽

Irina I. Nesterovich ◽

Vasilii I. Trophimov

Keyword(s):

Rheumatoid Arthritis ◽

Inflammatory Diseases ◽

Cardiovascular Complications ◽

Dark Field ◽

Joint Disease ◽

Endothelial Glycocalyx ◽

Leading Role ◽

Dark Field Microscopy ◽

Therapeutic Measures

Rheumatoid arthritis is the most common inflammatory joint disease and one of the most common chronic inflammatory diseases. The leading role in the pathogenesis of rheumatoid arthritis is the damage to the endothelial glycocalyx a thin dynamic layer of macromolecules located on the surface of the endothelium and consisting of proteoglycans, glycoproteins and glycosaminoglycans, which contributes to the maintenance of rheumatoid arthritis activity. Therefore, early detection of violations of the vasculr endothelium condition, especially the endothelial glycocalyx, will identify a group of patients with a poor prognosis. In this context, the method of dark-field microscopy may be promising. It allows non-invasive and in vivo assessment of the thickness of the sublingual endothelial glycocalyx, which will make it possible to search for new unconventional risk factors for the unfavorable course of rheumatoid arthritis and cardiovascular risk in these patients, as well as personalize treatment by developing a complex of preventive and therapeutic measures aimed at restoring endothelial function, reducing the risk of cardiovascular complications, disability and mortality from rheumatoid arthritis. The unique capabilities of this research method are demonstrated by the example of the clinical case.

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Active movement in vitro of bundle of microfilaments isolated from Nitella cell.

The Journal of Cell Biology ◽

10.1083/jcb.87.3.569 ◽

1980 ◽

Vol 87 (3) ◽

pp. 569-578 ◽

Cited By ~ 47

Author(s):

S Higashi-Fujime

Keyword(s):

Actin Filaments ◽

Dark Field ◽

Active Movement ◽

Artificial Medium ◽

Translational Movement ◽

Dark Field Microscopy ◽

Contrast Microscopy ◽

Subcortical Fibrils

Subcortical fibrils composed of bundles of F-actin filaments and endoplasmic filaments are responsible for endoplasmic streaming. It is reported here that these fibrils and filaments move actively in an artificial medium containing Mg-ATP and sucrose at neutral pH, when the medium was added to the cytoplasm squeezed out of the cell. The movement was observed by phase-contrast microscopy or dark-field microscopy and recorded on 16-mm film. Chains of chloroplasts linked by subcortical fibrils showed translational movement in the medium. Even after all chloroplasts and the endoplasm were washed away by perfusion with fresh medium, free fibrils and/or filaments (henceforth, referred to as fibers) not attached to chloroplasts continued travelling in the direction of the fiber orientation. Sometimes the fibers formed rings and rotated. Chloroplast chains and free fibers or rings continued moving for 5-30 min at about half the rate of the endoplasmic streaming in vivo. Calcium ion concentrations < 10(-7) M permitted movement to take place. Electron microscopy revealed that both fibers and rings were bundles of F-actin filaments that showed the same polarity after decoration with heavy meromyosin.

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