THE EFFECT OF MORINGA LEAF EXTRACT (MORINGA OLEIFERA L) AGAINST MOTILITY OF SPERMATOZOA MICE EXPOSED TO MONOSODIUM GLUTAMATE

Objective: Excessive use of MSG in everyday life can cause infertility to men. Exposure to MSG can cause cells to experience oxidative stress which subsequently triggers the generation of free radicals. Free radicals can be resisted with antioxidants. Material & Methods: This research was a laboratory experimental test with a post-test only control group design using male mice as the experimental object. The statistical test used the One Way ANOVA test. Results: In this research, 5 male mice were assigned into 4 groups and 1 control group. Using the One Way ANOVA test, it was found that there were significant differences in ≥ 2 treatment groups in the motility group of spermatozoa with active movement (p < 0.001) and the motility group of spermatozoa with weak movement (p = 0.036). The two groups showed significant differences. The Post hoc LSD test in the motility group with active movement showed significant differences between the control group with MSG treatment (p < 0.001) and the treatment group given Moringa leaves extract of 600 mg/kgW (p < 0.001) and 1200 mg/kgW (p < 0.001). Conclusion: Moringa leaves extract can effect the improvement of the motility of spermatozoa exposed to monosodium glutamate at a given dose of 300 mg/kgW and 600 mg/kgW.

Clinical Application of a Modified Local Transposition Flap Surgery in Repairing Fingertip Defects

Background: A modified local transposition flap surgery was performed for fingertip injuries, which allows a more significant transfer distance with good outcomes.Methods: The study collected patients who underwent parallelogram transposition flaps and V-Y flaps to repair fingertip defects from 2017 to 2020. 122 cases (122 fingers) were included in our study, The interval between the injury and operation was 5.78 h (the mean value was 4.7-8.4 h). All operations were performed by one surgical team, and the average operation time was 31.2 min.Record the operation time，two-point discrimination（2PD），Total Active Movement (TAM)and the MHQ (Michigan Hand Questionnaire) of the injured fingers to evaluate the therapeutic effect.Results: All parallelogram （Group A）and V-Y flaps （Group B）had survived postoperatively. There was no difference with operative duration and follow-up time in two groups. At last follow-up, there was no difference with the 2PD of the palmar part of the flaps and the TAM of injured figures in Group A and Group B . The MHQ summary scores in Group A were much higher than in Group B . Evaluation of the MHQ subscale performance showed that the overall hand function, activities of daily living, work performance and pain score had no differences, but aesthetics and satisfaction score was higher in Group A .Conclusions: The reconstruction using parallelogram flaps is a easier and more versatile treatment with better functions, less morbidity and better aesthetics. This method is a better choice for reconstruction of fingertip injury.

Effect of proprioceptive stimulation using a soft robotic glove on motor activation and brain connectivity in stroke survivors

Objective. Soft-robotic-assisted training may improve motor function during post-stroke recovery, but the underlying physiological changes are not clearly understood. We applied a single-session of intensive proprioceptive stimulation to stroke survivors using a soft robotic glove to delineate its short-term influence on brain functional activity and connectivity. Approach. In this study, we utilized task-based and resting-state functional magnetic resonance imaging (fMRI) to characterize the changes in different brain networks following a soft robotic intervention. Nine stroke patients with hemiplegic upper limb engaged in resting-state and motor-task fMRI. The motor tasks comprised two conditions: active movement of fingers (active task) and glove-assisted active movement using a robotic glove (glove-assisted task), both with visual instruction. Each task was performed using bilateral hands simultaneously or the affected hand only. The same set of experiments was repeated following a 30-minute treatment of continuous passive motion (CPM) using a robotic glove. Main results. On simultaneous bimanual movement, increased activation of supplementary motor area (SMA) and primary motor area (M1) were observed after CPM treatment compared to the pre-treatment condition, both in active and glove-assisted task. However, when performing the tasks solely using the affected hand, the phenomena of increased activity were not observed either in active or glove-assisted task. The comparison of the resting-state fMRI between before and after CPM showed the connectivity of the supramarginal gyrus and SMA was increased in the somatosensory network and salience network. Significance. This study demonstrates how passive motion exercise activates M1 and SMA in the post-stroke brain. The effective proprioceptive motor integration seen in bimanual exercise in contrast to the unilateral affected hand exercise suggests that the unaffected hemisphere might reconfigure connectivity to supplement damaged neural networks in the affected hemisphere. The somatosensory modulation rendered by the intense proprioceptive stimulation would affect the motor learning process in stroke survivors.

Effectiveness and Outcome of Early Weight Bearing in Ankle Fracture: A Systemic Review

Introduction: Ankle fractures are claimed to affect 70-185 patients out of 100,000 people each year. There is currently no unanimity among surgeons throughout the world on early versus restricted weight bearing in surgically treated trauma patients with fractures. The aim of this study was to systematically review the effectiveness and outcome of early weight bearing in ankle fracture as well as quality of life and pain. Methods: An electronic search was conducted of the databases EMBASE, PubMed, and The Cochrane Library, as well as Medline, Google Scholar to identify all articles related to the topic that have been published after 2006. Results: Initially, 2434 records were identified. Due to similarity and duplication, 1308 records were eliminated, and 1126 records were kept. Following the screening of the records and their subjects, 921 further unconnected records were eliminated. Then, 205 qualified full-text articles were selected, and 10 articles were included in qualitative analysis. Conclusion: According to the findings of this study, surgical therapy, both aggressive and minimal, appears to be a safe alternative for the elderly. Short-term advantages of early weight-bearing include a decreased incidence of thromboembolic disease. Active movement reduces joint stiffness while maintaining muscle mass. Post-operative mobility, quality of life, and post-operative early weight bearing status are considered as significant outcome indicators. Unsafe weight as tolerated after ankle surgery might be a safe and encouraging option. As a result, future research should include this possibilities.

Internal friction influence on the calculation results and rod system optimization under impulse action

An algorithm for calculating elastic rod systems under the action of impulse loads, using a complex model of internal friction in the material, has been developed and implemented in software. Very short (instantaneous) and extended in time impulses are considered as variants of impulse action. The importance of taking into account the vibration energy dissipation due to internal friction in the material of the structure is shown, considering impulse effects. The implemented software module is used to calculate the dynamic responses of the system in the search for the optimal solution of the control program for the selected variable parameters, target and restrictive functions. The problem of optimizing a flat frame system loaded with static and impulse loads has been posed and solved. An algorithm for finding an optimal solution is considered. Variants of dividing variable parameters into generalized groups are discussed. The minimum volume of material, spent on the structure, is taken as an optimality criterion. Analysis of the influence of the pulse duration of a given value on the calculation results without and with internal friction, as well as a comparative analysis of the optimal designs obtained without and with internal friction in the material and various tolerances for horizontal displacements. The results obtained indicate a significant effect of internal friction on the characteristics of the optimal design, especially with active movement restrictions.

Survival of femur fractures in wild stoats (Mustela erminea)

Stoats (Mustela erminea) are active hunters and, therefore, one might predict that any broken bones or other injuries impeding active movement would incur a serious risk of starvation. Dead stoats (n = 560) were collected from trappers operating predator control lines in three conservation areas of New Zealand from 1972–1978. Femurs were cleaned and examined for healed injuries and deformities. Five femurs from four stoats (one with both femurs injured) showed traumatic distortions following healing of complete breaks incurred during life. A further case recorded during post-eradication monitoring in 2010 on Rangitoto, an offshore island, is added. These data provide evidence that wild stoats have a remarkable capacity to tolerate catastrophic femur fractures. They can survive long enough, despite the implied limitation to their energetic hunting style, to permit full healing even though the result is a gross distortion of the femoral shaft.

Modulation of cutaneous responses in the cuneate nucleus of macaques during active movement

To achieve stable and precise movement execution, the sensorimotor system integrates exafferent sensory signals originating from interactions with the external world and reafferent signals caused by our own movements. This barrage of sensory information is regulated such that behaviorally relevant signals are boosted at the expense of irrelevant ones. For example, sensitivity to touch is reduced during movement - when cutaneous signals caused by skin stretch are expected and uninteresting - a phenomenon reflected in a decreased cutaneous responsiveness in thalamus and cortex. Some evidence suggests that movement gating of touch may originate from the cuneate nucleus (CN), the first recipient of signals from tactile nerve fibers along the dorsal columns medial lemniscal pathway. To test this possibility, we intermittently delivered mechanical pulses to the receptive fields (RFs) of identified cutaneous CN neurons as monkeys performed a reach-to-grasp task. As predicted, we found that the cutaneous responses of individual CN neurons were reduced during movement. However, this movement gating of cutaneous signals was observed for CN neurons with RFs on the arm but not those with RFs on the hand. We conclude that sensory gating occurs in the first processing stage along the somatosensory neuraxis and sculpts incoming signals according to their task relevance.

Evaluation of functional outcome of metacarpal fractures managed by operative techniques: a hospital based study

Background: Metacarpal fractures comprise approximately 35.5% of cases in daily emergencies, mostly due to road traffic accidents, fall, and assault. The main goal of treatment is to achieve the strong bony union without any functional disability. Aim of the study was evaluation of functional outcome of metacarpal fractures managed by operative techniques and to compare the efficacy of different operative techniques.Methods: A total of 50 patients managed by various operative techniques by using k-wires, screws and plates. Functional outcome was assessed by using total active movement (TAM) and disabilities of the arm, shoulder and hand (DASH) scoring system at 6th week, 3rd month and at 6th month.Results: A total 47 fractures united and three fractures mal-united at the time of final assessment. Overall excellent and good results were achieved in 94% cases.Conclusions: Our finding suggests that open reduction and internal fixation with k-wire is most preferable method among all other operative techniques.

3d Printed Customized Total Scapular Replacement With Constrained Shoulder Joint After Wide Resection For Scapula Chondrosarcoma Treatment: A Case Report

IntroductionTotal scapular replacement surgery with constrained reverse shoulder joint a complex surgery, especially in the selection of replacement materials. The advent of three-dimensional (3D) printing technique contribute significantly to the success of surgeryCase presentationA 62-year-old female patient discovered a scapular tumor at the end of 2017. She underwent curettage and bone graft surgery 3 times. The histopathological diagnosis was enchondroma. However, tumor recurrence and malignant transformation unfortunately presented later. She visited our center and total scapular replacement with constrained reverse shoulder joint was then performed. After 6 months, the patient's functional outcome was evaluated by using Musculoskeletal Tumour Society Score (MSTS) with a score of 26/30. There was no sign of local tumor recurrence. The functional of elbow and hand was preserved almost completely. The range of active movement of the shoulder was 60° for forward flexion and abduction, and 45° for backward flexion.DiscussionDespite the follow-up time was still short, the use of the constrained shoulder joint with the applications of patient-specific 3D printing showed very positive outcomes. Patient was able to start rehabilitative early without any problems. More follow-up time would be needed to evaluate the long-term effectiveness of this method.ConclusionTotal scapular replacement with constrained reverse shoulder joint using 3D printing technology is a suitable option for the patients with scapular tumor invading the muscles of the rotator cuff.

Screening of potential donors for immunoglobulins M and G to new coronavirus infection

This study presents the results of the examination of potential donors of blood and its components for immunoglobulins M and G to patients with coronavirus disease 2019 (COVID-19) living in St. Petersburg. A total of 6782 people aged 1824 years were evaluated, which accounted for 2.07% of the regions population (326 760 people) of this age group. The study was carried out in the spring and autumn of 2020. A negative result (absence of antibodies) was obtained in 93.5% of the participants. The rates of immunoglobulins M and M + G were 0.58% and 4.18%, respectively, in the spring and autumn. Moreover, the number of participants who had immunoglobulins M and G + M in the autumn period was four times higher than the indicators of the spring period, which indicated greater infection activities in the population during this period. This is most likely due to the active movement of the population in the summer. When comparing the rates of COVID-19 infection and the frequency of occurrence in donors of the same age, markers of human immunodeficiency virus 1 and 2 and hepatitis B and C in 2020 (0.024, 0.012 and 0.13%, respectively) indicate the urgency of the problem of donor selection during blood services, especially during a difficult epidemiological situation because of COVID-19. Along with organizational measures for the selection of donors (e.g., attracting individuals from organized groups in which there are no signs of an unfavorable epidemiological situation to donation), mandatory testing of potential donors for immunoglobulins M and G should be considered.