Ultrafast dynamics in a live cell irradiated by femtosecond laser pulses

Owning to excellent optical properties and high biocompatibility carbon dots (CDs) have drawn increasing attention and have been widely applied as imaging agents for various bio-applications. Here we report a strategy for live-cell fluorescent bioimaging based on in situ synthesis of CDs within cells by tightly focused femtosecond laser pulses. Laser-produced carbon dots exhibit bright excitation-dependent fluorescence and are highly two-photon active under near infrared femtosecond excitation, thus demonstrating a potential for two-photon fluorescence imaging. The Raman spectra of fluorescent centers show strong D (1350 cm-1) and G (1590 cm-1) bands, thus suggesting that they are composed of carbon dots with sp2-hybridized core. Using Mouse GV oocytes as a model system we examine cytotoxicity and demonstrate the possibility of long-term fluorescent intracellular tracking of the laser-produced CDs. Created virtually in any point of the live cell, CD-based fluorescent μm-sized markers demonstrate high structural stability and retain bright fluorescence many hours after formation. Our results point to laser-produced fluorescent CDs as a highly-potent tool for cell cycle tracking, culture cell marking and probing intracellular movements.

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Modelling of the ultrafast dynamics and surface plasmon properties of silicon upon irradiation with mid-IR femtosecond laser pulses

Physical Review B ◽

10.1103/physrevb.99.195201 ◽

2019 ◽

Vol 99 (19) ◽

Cited By ~ 10

Author(s):

E. Petrakakis ◽

G. D. Tsibidis ◽

E. Stratakis

Keyword(s):

Femtosecond Laser ◽

Surface Plasmon ◽

Laser Pulses ◽

Femtosecond Laser Pulses ◽

Ultrafast Dynamics

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Ultrafast dynamics of periodic nanostructure formation on diamondlike carbon films irradiated with femtosecond laser pulses

Applied Physics Letters ◽

10.1063/1.2374858 ◽

2006 ◽

Vol 89 (19) ◽

pp. 191902 ◽

Cited By ~ 57

Author(s):

Godai Miyaji ◽

Kenzo Miyazaki

Keyword(s):

Femtosecond Laser ◽

Laser Pulses ◽

Carbon Films ◽

Femtosecond Laser Pulses ◽

Ultrafast Dynamics ◽

Nanostructure Formation ◽

Diamondlike Carbon ◽

Periodic Nanostructure

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Influence of Trap States on the Kinetics of Luminescence and Induced Light Absorption by Si Nanoparticles in a SiO2 Matrix at Their Excitation with Femtosecond Laser Pulses

Ukrainian Journal of Physics ◽

10.15407/ujpe58.01.0020 ◽

2013 ◽

Vol 58 (1) ◽

pp. 20-25

Author(s):

V.M. Kadan ◽

◽

I.Z. Indutnyi ◽

V.A. Dan’ko ◽

P.E. Shepelyavyi ◽

...

Keyword(s):

Femtosecond Laser ◽

Light Absorption ◽

Laser Pulses ◽

Femtosecond Laser Pulses ◽

Trap States ◽

Sio2 Matrix ◽

Si Nanoparticles ◽

Kinetics Of

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Inorganic-organic Hybrid Materials for Real 3-D Sub-νm Lithography

MRS Proceedings ◽

10.1557/proc-780-y4.12 ◽

2003 ◽

Vol 780 ◽

Cited By ~ 1

Author(s):

R. Houbertz ◽

J. Schulz ◽

L. Fröhlich ◽

G. Domann ◽

M. Popall ◽

...

Keyword(s):

Femtosecond Laser ◽

Sol Gel ◽

Laser Pulses ◽

Femtosecond Laser Pulses ◽

Hybrid Polymer ◽

Organic Hybrid ◽

Two Photon ◽

Applied Technology ◽

Scale Structures ◽

Sol Gel Synthesis

AbstractReal 3-D sub-νm lithography was performed with two-photon polymerization (2PP) using inorganic-organic hybrid polymer (ORMOCER®) resins. The hybrid polymers were synthesized by hydrolysis/polycondensation reactions (modified sol-gel synthesis) which allows one to tailor their material properties towards the respective applications, i.e., dielectrics, optics or passivation. Due to their photosensitive organic functionalities, ORMOCER®s can be patterned by conventional photo-lithography as well as by femtosecond laser pulses at 780 nm. This results in polymerized (solid) structures where the non-polymerized parts can be removed by conventional developers.ORMOCER® structures as small as 200 nm or even below were generated by 2PP of the resins using femtosecond laser pulses. It is demonstrated that ORMOCER®s have the potential to be used in components or devices built up by nm-scale structures such as, e.g., photonic crystals. Aspects of the materials in conjunction to the applied technology are discussed.

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