Simultaneously reconstructing fluorescent yield and lifetime from time-resolved transmittances of a small-animal-sized phantom

2009 ◽  
Author(s):  
Feng Gao ◽  
Limin Zhang ◽  
Patrick Poulet ◽  
Jiao Li ◽  
Huijuan Zhao ◽  
...  
Keyword(s):  
Small Animal ◽  
Time Resolved

Simultaneous fluorescence yield and lifetime tomography from time-resolved transmittances of small-animal-sized phantom

2010 ◽  
Vol 49 (16) ◽  
pp. 3163 ◽  
Author(s):  
Feng Gao ◽  
Jiao Li ◽  
Limin Zhang ◽  
Patrick Poulet ◽  
Huijuan Zhao ◽  
...  
Keyword(s):  
Small Animal ◽  
Fluorescence Yield ◽  
Time Resolved

Simultaneously reconstructing fluorescent yield and lifetime from time-resolved transmittances of a small-animal-sized phantom

2009 ◽  
Author(s):  
Feng Gao ◽  
Limin Zhang ◽  
Patrick Poulet ◽  
Jiao Li ◽  
Huijuan Zhao ◽  
...  
Keyword(s):  
Small Animal ◽  
Time Resolved

scholarly journals High Time-Resolved Cardiac Functional Imaging Using Temporal Regularization for Small Animal on a Clinical 3T Scanner

2012 ◽  
Vol 59 (4) ◽  
pp. 929-935 ◽  
Author(s):  
B. M. A. Delattre ◽  
D. Van De Ville ◽  
V. Braunersreuther ◽  
C. Pellieux ◽  
J-N Hyacinthe ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Small Animal ◽  
High Time ◽  
Time Resolved

Microtubule nucleation sequence studied by time-resolved x-ray scattering and electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 766-767
Author(s):  
Eva-Maria Mandelkow ◽  
Eckhard Mandelkow ◽  
Joan Bordas
Keyword(s):  
Electron Microscopy ◽  
Dynamic Equilibrium ◽  
Time Resolved ◽  
X Ray ◽  
Additional Information ◽  
X Ray Scattering ◽  
Low Concentrations ◽  
Microtubule Growth ◽  
Ray Patterns ◽  
Ray Scattering

When a solution of microtubule protein is changed from non-polymerising to polymerising conditions (e.g. by temperature jump or mixing with GTP) there is a series of structural transitions preceding microtubule growth. These have been detected by time-resolved X-ray scattering using synchrotron radiation, and they may be classified into pre-nucleation and nucleation events. X-ray patterns are good indicators for the average behavior of the particles in solution, but they are difficult to interpret unless additional information on their structure is available. We therefore studied the assembly process by electron microscopy under conditions approaching those of the X-ray experiment. There are two difficulties in the EM approach: One is that the particles important for assembly are usually small and not very regular and therefore tend to be overlooked. Secondly EM specimens require low concentrations which favor disassembly of the particles one wants to observe since there is a dynamic equilibrium between polymers and subunits.

Electron Channeling Patterns

1977 ◽  
Vol 35 ◽  
pp. 12-13
Author(s):  
David C. Joy
Keyword(s):  
Electron Diffraction ◽  
Incidence Angle ◽  
Photographic Plate ◽  
Diffraction Effect ◽  
Angle Of Incidence ◽  
Bulk Single Crystal ◽  
Time Resolved ◽  
Electron Channeling ◽  
Spatially Resolved ◽  
Large Bulk

Electron channeling patterns (ECP) were first found by Coates (1967) while observing a large bulk, single crystal of silicon in a scanning electron microscope. The geometric pattern visible was shown to be produced as a result of the changes in the angle of incidence, between the beam and the specimen surface normal, which occur when the sample is examined at low magnification (Booker, Shaw, Whelan and Hirsch 1967).A conventional electron diffraction pattern consists of an angularly resolved intensity distribution in space which may be directly viewed on a fluorescent screen or recorded on a photographic plate. An ECP, on the other hand, is produced as the result of changes in the signal collected by a suitable electron detector as the incidence angle is varied. If an integrating detector is used, or if the beam traverses the surface at a fixed angle, then no channeling contrast will be observed. The ECP is thus a time resolved electron diffraction effect. It can therefore be related to spatially resolved diffraction phenomena by an application of the concepts of reciprocity (Cowley 1969).

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