Two-Photon Imaging of Cortical Microvascular Blood Flow in Response to Single Vessel Occlusion

2005 ◽  
Author(s):  
N. Nishimura ◽  
C. B. Schaffer ◽  
B. Friedman ◽  
P. S. Tsai ◽  
P. D. Lyden ◽  
...  
Keyword(s):  
Blood Flow ◽  
Microvascular Blood Flow ◽  
Vessel Occlusion ◽  
Two Photon ◽  
Single Vessel ◽  
Photon Imaging ◽  
Two Photon Imaging

Two-Photon Imaging of Capillary Blood Flow in Olfactory Bulb Glomeruli

2009 ◽  
pp. 81-91 ◽  
Author(s):  
Pascale Tiret ◽  
Emmanuelle Chaigneau ◽  
Jérôme Lecoq ◽  
Serge Charpak
Keyword(s):  
Blood Flow ◽  
Olfactory Bulb ◽  
Capillary Blood ◽  
Capillary Blood Flow ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals In Vivo Two-Photon Imaging of Axonal Dieback, Blood Flow and Calcium Influx withMethylprednisolone Therapy after Spinal Cord Injury

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Peifu Tang ◽  
Yiling Zhang ◽  
Chao Chen ◽  
Xinran Ji ◽  
Furong Ju ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Blood Flow ◽  
Calcium Influx ◽  
Two Photon ◽  
Cord Injury ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Two-photon imaging of capillary blood flow in olfactory bulb glomeruli

2003 ◽  
Vol 100 (22) ◽  
pp. 13081-13086 ◽  
Author(s):  
E. Chaigneau ◽  
M. Oheim ◽  
E. Audinat ◽  
S. Charpak
Keyword(s):  
Blood Flow ◽  
Olfactory Bulb ◽  
Capillary Blood ◽  
Capillary Blood Flow ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

Two-Photon Imaging of Blood Flow in the Rat Cortex

2013 ◽  
Vol 2013 (8) ◽  
pp. pdb.prot076513 ◽  
Author(s):  
Jonathan D. Driscoll ◽  
Andy Y. Shih ◽  
Patrick J. Drew ◽  
Gert Cauwenberghs ◽  
David Kleinfeld
Keyword(s):  
Blood Flow ◽  
Rat Cortex ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vessels

2011 ◽  
Vol 17 (7) ◽  
pp. 893-898 ◽  
Author(s):  
Jérôme Lecoq ◽  
Alexandre Parpaleix ◽  
Emmanuel Roussakis ◽  
Mathieu Ducros ◽  
Yannick Goulam Houssen ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Vessels ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Two-Photon Imaging of Cortical Surface Microvessels Reveals a Robust Redistribution in Blood Flow after Vascular Occlusion

PLoS Biology ◽  
2006 ◽  
Vol 4 (2) ◽  
pp. e22 ◽  
Author(s):  
Chris B Schaffer ◽  
Beth Friedman ◽  
Nozomi Nishimura ◽  
Lee F Schroeder ◽  
Philbert S Tsai ◽  
...  
Keyword(s):  
Blood Flow ◽  
Vascular Occlusion ◽  
Cortical Surface ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Depth-targeted intracortical microstroke by two-photon photothrombosis in rodent brain

2021 ◽  
Author(s):  
Masahiro Fukuda ◽  
Toshio Suda ◽  
Takayoshi Matsumura ◽  
Hajime Hirase
Keyword(s):  
Blood Flow ◽  
Rose Bengal ◽  
Blood Stream ◽  
Target Vessel ◽  
Vessel Occlusion ◽  
Two Photon ◽  
Single Vessel ◽  
Cortical Parenchyma ◽  
Photon Excitation ◽  
Two Photon Excitation

Significance: Photothrombosis is a widely used model of ischemic stroke in rodent experiments. In the photothromboris model, the photosensitizer Rose Bengal is systemically introduced to the blood stream and activated by green light to induce aggregation of platelets that eventually cause vessel occlusion. Since the activation of Rose Bengal is a one-photon phenomenon and the molecules in the illuminated area (light path) are subject to excitation, targeting of thrombosis is unspecific especially in the depth dimension. We have developed a photothrombosis protocol that can target a single vessel in the cortical parenchyma by two-photon excitation. Aim: We aim to induce a thrombotic stroke in the cortical parenchyma by two-photon activation of Rose Bengal so that we confine photothrombosis within a vessel of a target depth. Approach: FITC-dextran is injected into the blood stream to visualize the cerebral blood flow in anesthetized adult mice with a cranial window. After a target vessel is chosen by two-photon imaging (950 nm), Rose Bengal is injected into the blood stream. The scanning wavelength is changed to 720 nm and photothrombosis was induced by scanning the target vessel. Results: Two-photon depth-targeted single vessel photothrombosis was achieved with a success rate of 84.9+/-1.7% within 80 s. Attempts without Rose Bengal (i.e., only with FITC) did not result in photothrombosis at the excitation wavelength of 720 nm. Conclusions: We described a protocol that achieves depth-targeted single vessel photothrombosis by two-photon excitation. Simultaneous imaging of blood flow in the targeted vessel using FITC dextran enabled the confirmation of vessel occlusion and prevention of excess irradiation that possibly induces unintended photodamage.

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