Detection of target molecules on the single molecule level using confocal fluorescence microscopy in combination with microelectophoresis

2001 ◽  
Author(s):  
Andreas Schulz ◽  
V. Buschmann ◽  
T. Hubner ◽  
H. Neuweiler ◽  
M. Sauer ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Confocal Fluorescence Microscopy ◽  
Single Molecule Level ◽  
Confocal Fluorescence ◽  
Target Molecules

scholarly journals Photoinduced Electron Transfer of Oxazine 1/TiO2Nanoparticles at Single Molecule Level by Using Confocal Fluorescence Microscopy

Langmuir ◽  
2010 ◽  
Vol 26 (11) ◽  
pp. 9050-9060 ◽  
Author(s):  
Yi-Ju Chen ◽  
Hsin-Yu Tzeng ◽  
Hsiu-Fang Fan ◽  
Ming-Shiang Chen ◽  
Jer-Shing Huang ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Photoinduced Electron Transfer ◽  
Confocal Fluorescence Microscopy ◽  
Single Molecule Level ◽  
Confocal Fluorescence ◽  
Oxazine 1

Measuring Diffusion in Nanoscale Environments with Single‐Molecule Confocal Fluorescence Microscopy

2004 ◽  
Vol 37 (2) ◽  
pp. 129-149 ◽  
Author(s):  
Daniel L. Burden ◽  
Peter K. Walhout ◽  
John T. Elliott ◽  
Emily L. Chandler ◽  
Roger G. Scharf ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence

Real-time observation of single molecules by confocal fluorescence microscopy

1995 ◽  
Vol 53 ◽  
pp. 60-61
Author(s):  
Shuming Nie ◽  
Daniel T. Chiu ◽  
Richard N. Zare
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Single Molecule ◽  
High Efficiency ◽  
Spherical Aberration ◽  
Correlation Spectroscopy ◽  
Optical Diffraction ◽  
Confocal Fluorescence Microscopy ◽  
Scanning Tunneling ◽  
Confocal Fluorescence

The ability to detect, identify, and manipulate individual molecules offer exciting possibilities in many fields, including chemical analysis, materials research, and the biological sciences. A particularly powerful approach is to combine the exquisite sensitivity of laser-induced fluorescence and the spatial localization and imaging capabilities of diffraction-limited or near-field optical microscopes. Unlike scanning tunneling microscopy (STM) and atomic force microscopy (AFM), which lack molecular specificity, optical spectroscopy and microscopy techniques can be used for real-time monitoring and molecular identification at nanometer dimensions or in ultrasmall volumes.We report the use of confocal fluorescence microscopy coupled with a diffraction-limit laser beam and a high-efficiency photodiode for real-time detection of single fluorescent molecules in solution at room temperature. Rigler and Eigen have also demonstrated single-molecule detection with a confocal microscope and fluorescence correlation spectroscopy. The probe (or sampling) volume is effectively an elongated cylinder, with its radius being determined by optical diffraction and length by spherical aberration.

Functional Fluorescence Microscopy Imaging: Quantitative Scanning-Free Confocal Fluorescence Microscopy for the Characterization of Fast Dynamic Processes in Live Cells

2019 ◽  
Vol 91 (17) ◽  
pp. 11129-11137 ◽  
Author(s):  
Aleksandar J. Krmpot ◽  
Stanko N. Nikolić ◽  
Sho Oasa ◽  
Dimitrios K. Papadopoulos ◽  
Marco Vitali ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Confocal Fluorescence Microscopy ◽  
Live Cells ◽  
Dynamic Processes ◽  
Microscopy Imaging ◽  
Confocal Fluorescence ◽  
Fast Dynamic

Choline and phosphatidylcholine fluorescent derivatives localization in carcinoma cells studied by laser scanning confocal fluorescence microscopy

2005 ◽  
Vol 41 (10) ◽  
pp. 1453-1459 ◽  
Author(s):  
Anna M. Villa ◽  
Elvira Caporizzo ◽  
Antonio Papagni ◽  
Luciano Miozzo ◽  
Paola Del Buttero ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Laser Scanning ◽  
Carcinoma Cells ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence ◽  
Fluorescent Derivatives

scholarly journals Analysis of Polyhydroxyalkanoates Granules in Haloferax mediterranei by Double-Fluorescence Staining with Nile Red and SYBR Green by Confocal Fluorescence Microscopy

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1582
Author(s):  
Verónica Cánovas ◽  
Salvador Garcia-Chumillas ◽  
Fuensanta Monzó ◽  
Lorena Simó-Cabrera ◽  
Carmen Fernández-Ayuso ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Culture Media ◽  
Nile Red ◽  
Sybr Green ◽  
Confocal Fluorescence Microscopy ◽  
Fluorescence Staining ◽  
Haloferax Mediterranei ◽  
Optimized Protocol ◽  
Confocal Fluorescence ◽  
Pha Production

Haloferaxmediterranei is a haloarchaeon of high interest in biotechnology because it produces and mobilizes intracellular polyhydroxyalkanoate (PHA) granules during growth under stress conditions (limitation of phosphorous in the culture media), among other interesting metabolites (enzymes, carotenoids, etc.). The capability of PHA production by microbes can be monitored with the use of staining-based methods. However, the staining of haloarchaea cells is a challenging task; firstly, due to the high ionic strength of the medium, which is inappropriate for most of dyes, and secondly, due to the low permeability of the haloarchaea S-layer to macromolecules. In this work, Haloferax mediterranei is used as a halophilic archaeon model to describe an optimized protocol for the visualization and analysis of intracellular PHA granules in living cells. The method is based on double-fluorescence staining using Nile red and SYBR Green by confocal fluorescence microscopy. Thanks to this method, the capability of PHA production by new haloarchaea isolates could be easily monitored.

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