Real-Time Monitoring of Atmospheric Aerosol at New Haven, CT, for Fluorescence Spectra, Particle Size and Concentration

2007 ◽  
Author(s):  
Yong-Le Pan ◽  
Richard K. Chang ◽  
Ronald G. Pinnick ◽  
Steven C. Hill
Keyword(s):  
Particle Size ◽  
Real Time ◽  
Atmospheric Aerosol ◽  
Fluorescence Spectra ◽  
New Haven ◽  
Real Time Monitoring

scholarly journals Enzymatic degradation of maize shoots: monitoring of chemical and physical changes reveals different saccharification behaviors

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Cécile Barron ◽  
Marie-Françoise Devaux ◽  
Loïc Foucat ◽  
Xavier Falourd ◽  
Rachelle Looten ◽  
...  
Keyword(s):  
Particle Size ◽  
Real Time ◽  
Enzymatic Degradation ◽  
Medium Size ◽  
Molecular Heterogeneity ◽  
Real Time Monitoring ◽  
Tissue Heterogeneity ◽  
Sugar Release ◽  
Physical Changes ◽  
Number Of Particles

Abstract Background The recalcitrance of lignocellulosics to enzymatic saccharification has been related to many factors, including the tissue and molecular heterogeneity of the plant particles. The role of tissue heterogeneity generally assessed from plant sections is not easy to study on a large scale. In the present work, dry fractionation of ground maize shoot was performed to obtain particle fractions enriched in a specific tissue. The degradation profiles of the fractions were compared considering physical changes in addition to chemical conversion. Results Coarse, medium and fine fractions were produced using a dry process followed by an electrostatic separation. The physical and chemical characteristics of the fractions varied, suggesting enrichment in tissue from leaves, pith or rind. The fractions were subjected to enzymatic hydrolysis in a torus reactor designed for real-time monitoring of the number and size of the particles. Saccharification efficiency was monitored by analyzing the sugar release at different times. The lowest and highest saccharification yields were measured in the coarse and fine fractions, respectively, and these yields paralleled the reduction in the size and number of particles. The behavior of the positively- and negatively-charged particles of medium-size fractions was contrasted. Although the amount of sugar release was similar, the changes in particle size and number differed during enzymatic degradation. The reduction in the number of particles proceeded faster than that of particle size, suggesting that degradable particles were degraded to the point of disappearance with no significant erosion or fragmentation. Considering all fractions, the saccharification yield was positively correlated with the amount of water associated with [5–15 nm] pore size range at 67% moisture content while the reduction in the number of particles was inversely correlated with the amount of lignin. Conclusion Real-time monitoring of sugar release and changes in the number and size of the particles clearly evidenced different degradation patterns for fractions of maize shoot that could be related to tissue heterogeneity in the plant. The biorefinery process could benefit from the addition of a sorting stage to optimise the flow of biomass materials and take better advantage of the heterogeneity of the biomass.

scholarly journals Enzymatic Degradation of Maize Shoots: Monitoring of Chemical and Physical Changes Reveals Different Saccharification Behaviors

2020 ◽  
Author(s):  
Cécile Barron ◽  
Marie-Françoise Devaux ◽  
Loïc Foucat ◽  
Xavier Falourd ◽  
Rachelle Looten ◽  
...  
Keyword(s):  
Particle Size ◽  
Real Time ◽  
Enzymatic Degradation ◽  
Medium Size ◽  
Molecular Heterogeneity ◽  
Real Time Monitoring ◽  
Tissue Heterogeneity ◽  
Sugar Release ◽  
Physical Changes ◽  
Number Of Particles

Abstract Background The recalcitrance of lignocellulosics to enzymatic saccharification has been related to many factors, including the tissue and molecular heterogeneity of the plant particles. The role of tissue heterogeneity generally assessed from plant sections is not easy to study on a large scale. In the present work, dry fractionation of ground maize shoot was performed to obtain particle fractions enriched in specific tissue or molecules. The degradation profiles of the fractions were compared considering physical changes in addition to chemical conversion.Results Coarse, medium and fine fractions were produced using a dry process followed by an electrostatic separation. The physical and chemical characteristics of the fractions varied, suggesting enrichment in tissue from leaves, pith or rind. The fractions were subjected to seven hours of enzymatic hydrolysis in a torus reactor designed for real-time monitoring of the number and size of the particles. Saccharification efficiency was monitored by analyzing the sugar content of hydrolysate samples collected at different times. The lowest and highest saccharification yields were measured in the coarse and fine fractions, respectively, and these yields paralleled the reduction in the size and number of particles. The behavior of the positively- and negatively-charged particles of medium-size fractions was contrasted. Although the amount of sugar release was similar, the changes in particle size and number differed during enzymatic degradation. The reduction in the number of particles proceeded faster than that of particle size, suggesting that degradable particles were degraded to the point of disappearance with no significant erosion or fragmentation. Considering all fractions, the saccharification yield was positively correlated with the amount of water associated with [5-15 nm] pore size range at 67% moisture content while the reduction in the number of particles was inversely correlated with the amount of lignin. Lignin could act in maintaining the macroscopic structure of particles without necessarily prohibitive effects on the accessibility of enzymes to their substrates.Conclusion Real-time monitoring of sugar release and changes in the number and size of the particles clearly evidenced different degradation patterns for fractions of maize shoot that could be related to tissue heterogeneity in the plant.

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