Silica Colloidal Crystals as Porous Substrates for Total Internal Reflection Fluorescence Microscopy of Live Cells

2008 ◽  
Vol 62 (6) ◽  
pp. 611-616 ◽  
Author(s):  
Tomika R. C. Velarde ◽  
Mary J. Wirth
Keyword(s):  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Colloidal Crystals ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Live Cells ◽  
Porous Substrates ◽  
Silica Colloidal Crystals

Visualizing membrane trafficking using total internal reflection fluorescence microscopy

2003 ◽  
Vol 31 (4) ◽  
pp. 819-823 ◽  
Author(s):  
V. Beaumont
Keyword(s):  
Fluorescence Microscopy ◽  
Membrane Trafficking ◽  
Cell Biology ◽  
Total Internal Reflection ◽  
Optical Resolution ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Live Cells ◽  
Excitable Cells ◽  
Cellular Processes

There is a dizzying array of fluorescent probes now commercially available to monitor cellular processes, and advances in molecular biology have highlighted the ease with which proteins can now be labelled with fluorophores without loss of functionality. This has led to an explosion in the popularity of fluorescence microscopy techniques. One such specialized technique, total internal reflection fluorescence microscopy (TIR-FM), is ideally suited to gaining insight into events occurring at, or close to, the plasma membrane of live cells with excellent optical resolution. In the last few years, the application of TIR-FM to membrane trafficking events in both non-excitable and excitable cells has been an area of notable expansion and fruition. This review gives a brief overview of that literature, with emphasis on the study of the regulation of exocytosis and endocytosis in excitable cells using TIR-FM. Finally, recent applications of TIR-FM to the study of cellular processes at the molecular level are discussed briefly, providing promise that the future of TIR-FM in cell biology will only get brighter.

A perfusable microfluidic device with on-chip total internal reflection fluorescence microscopy (TIRFM) for in situ and real-time monitoring of live cells

2012 ◽  
Vol 14 (4) ◽  
pp. 791-797 ◽  
Author(s):  
Ryuji Yokokawa ◽  
Yuko Kitazawa ◽  
Kyohei Terao ◽  
Atsuhito Okonogi ◽  
Isaku Kanno ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Microfluidic Device ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Live Cells ◽  
Real Time Monitoring ◽  
On Chip

Nanograting-based plasmon enhancement for total internal reflection fluorescence microscopy of live cells

2008 ◽  
Vol 20 (1) ◽  
pp. 015202 ◽  
Author(s):  
Kyujung Kim ◽  
Dong Jun Kim ◽  
Eun-Jin Cho ◽  
Jin-Suck Suh ◽  
Yong-Min Huh ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Live Cells ◽  
Plasmon Enhancement

Multidimensional single-molecule imaging in live cells using total-internal-reflection fluorescence microscopy

2006 ◽  
Vol 31 (14) ◽  
pp. 2157 ◽  
Author(s):  
S. E. D. Webb ◽  
S. R. Needham ◽  
S. K. Roberts ◽  
M. L. Martin-Fernandez
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Live Cells ◽  
Single Molecule Imaging

scholarly journals Fast high-resolution 3D total internal reflection fluorescence microscopy by incidence angle scanning and azimuthal averaging

2014 ◽  
Vol 111 (48) ◽  
pp. 17164-17169 ◽  
Author(s):  
Jérôme Boulanger ◽  
Charles Gueudry ◽  
Daniel Münch ◽  
Bertrand Cinquin ◽  
Perrine Paul-Gilloteaux ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Incidence Angle ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence

scholarly journals Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology

2013 ◽  
Vol 102 (19) ◽  
pp. 193702 ◽  
Author(s):  
Minghan Chen ◽  
Natalya V. Zaytseva ◽  
Qi Wu ◽  
Min Li ◽  
Ye Fang
Keyword(s):  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Receptor Pharmacology
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