Important Aspects concerning the Quantification of Biomolecules by Time-of-Flight Secondary-Ion Mass Spectrometry

1996 ◽  
Vol 50 (2) ◽  
pp. 161-166 ◽  
Author(s):  
David C. Muddiman ◽  
Anthony J. Nicola ◽  
Andrew Proctor ◽  
David M. Hercules
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Dynamic Range ◽  
Dead Time ◽  
Time Of Flight ◽  
Internal Standard ◽  
Use Of Time ◽  
Ion Mass Spectrometry ◽  
Quantitative Results ◽  
Secondary Ion

Fundamental aspects regarding the use of time-of-flight secondary-ion mass spectrometry (TOF-SIMS) as a quantitative tool for the analysis of organic compounds are reported. The following factors are discussed: (1) the use of Poisson's law to correct for dead-time in single-ion data collection; (2) practical considerations concerning the analysis of “real world” samples; and (3) the effect of the etching process on the reproducibility of the intensity ratio (analyte/internal standard) of Ag-cationized species. To evaluate the importance of these factors, we used cocaine and cyclosporin A (CsA) as analytes because they show protonated and Ag-cationized species, respectively, in their SIMS spectra. Correction for detector dead-time using Poisson's law of single-ion counting expanded the dynamic range for cocaine by ∼2 orders of magnitude. For analyses requiring only a small dynamic range (i.e., CsA), the correction improved the % RSD of the slope from 2.43 to 0.87%. The maximum secondary-ion (SI) yield of CsA (Ag-cationized species) occurs at a CsA concentration ∼3 orders of magnitude higher than the therapeutic levels in blood (25–2000 ng/mL). It is discussed how this problem should be addressed. Analysis of variance (ANOVA) indicates that Ag substrates must be etched under identical conditions to obtain quantitative results when species requiring cationization are being analyzed.

Smith-Lemli-Opitz syndrome diagnosed by using time-of-flight secondary-ion mass spectrometry

1995 ◽  
Vol 41 (4) ◽  
pp. 548-552 ◽  
Author(s):  
U Seedorf ◽  
M Fobker ◽  
R Voss ◽  
K Meyer ◽  
F Kannenberg ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Ion Mass Spectrometry ◽  
Time Of Flight ◽  
Internal Standard ◽  
Laboratory Diagnosis ◽  
Tof Sims ◽  
Ion Mass Spectrometry ◽  
Opitz Syndrome ◽  
Secondary Ion

Abstract We describe a rapid and sensitive method involving time-of-flight secondary-ion mass spectrometry (TOF-SIMS) for specific laboratory diagnosis of the Smith-Lemli-Opitz syndrome, which is characterized by massive (approximately 1000-fold) accumulation of the biosynthetic cholesterol precursor 7-dehydrocholesterol. Minute amounts of blood (1-50 microL) were extracted with n-hexane, and aliquots were analyzed by TOF-SIMS. 7-Dehydrocholesterol and its isomers were detected at 491.3 mass units ([M + 107Ag]+) and cholesterol at 495.3 mass units ([M + 109Ag]+). Quantitation of 7-dehydrocholesterol and cholesterol was achieved after saponification and addition of stigmasterol as internal standard. Whereas 7-dehydrocholesterol and isomeric dehydrocholesterol were not detectable in controls, the patients revealed concentrations ranging between 0.84 and 1.25 mmol/L. Comparison with results obtained by gas chromatography indicated that quantitation by TOF-SIMS yielded the sum of 7-dehydrocholesterol, isomeric dehydrocholesterol II, and sterol III, the latter two also being increased in the patients. Consistent with quantitation by gas chromatography, the cholesterol concentrations in the patients ranged between 1.54 and 2.12 mmol/L (controls: 6.10 +/- 1.37 mmol/L).

ChemInform Abstract: Use of Time of Flight Static-Secondary Ion Mass Spectrometry in Peptide Synthesis on Solid Support.

ChemInform ◽  
2010 ◽  
Vol 33 (13) ◽  
pp. no-no
Author(s):  
Christine Enjalbal ◽  
Gilles Subra ◽  
Robert Combarieu ◽  
Jean Martinez ◽  
Jean-Louis Aubagnac
Keyword(s):  
Mass Spectrometry ◽  
Peptide Synthesis ◽  
Secondary Ion Mass Spectrometry ◽  
Time Of Flight ◽  
Solid Support ◽  
Use Of Time ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

scholarly journals Determining the chronology of deposition of natural fingermarks and inks on paper using secondary ion mass spectrometry

The Analyst ◽  
2014 ◽  
Vol 139 (18) ◽  
pp. 4641-4653 ◽  
Author(s):  
Nicola Attard-Montalto ◽  
Jesús J. Ojeda ◽  
Alan Reynolds ◽  
Mahado Ismail ◽  
Melanie Bailey ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Time Of Flight ◽  
Paper Surface ◽  
Use Of Time ◽  
Tof Sims ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

This study thoroughly explores the use of time-of-flight secondary ion mass spectrometry (ToF-SIMS) for determining the deposition sequence of fingermarks and ink on a porous paper surface.

Applications of Time-OF-Flight Secondary Ion Mass Spectrometry (TOF-SIMS)

1990 ◽  
Vol 48 (2) ◽  
pp. 308-309
Author(s):  
Bruno Schueler ◽  
Robert W. Odom
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Structural Information ◽  
Time Of Flight ◽  
Biological Tissues ◽  
Polymer Surfaces ◽  
Tof Sims ◽  
Secondary Ions ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) provides unique capabilities for elemental and molecular compositional analysis of a wide variety of surfaces. This relatively new technique is finding increasing applications in analyses concerned with determining the chemical composition of various polymer surfaces, identifying the composition of organic and inorganic residues on surfaces and the localization of molecular or structurally significant secondary ions signals from biological tissues. TOF-SIMS analyses are typically performed under low primary ion dose (static SIMS) conditions and hence the secondary ions formed often contain significant structural information.This paper will present an overview of current TOF-SIMS instrumentation with particular emphasis on the stigmatic imaging ion microscope developed in the authors’ laboratory. This discussion will be followed by a presentation of several useful applications of the technique for the characterization of polymer surfaces and biological tissues specimens. Particular attention in these applications will focus on how the analytical problem impacts the performance requirements of the mass spectrometer and vice-versa.

Optics-Free Visualization of Proteins in Single Cells by Time of Flight-Secondary Ion Mass Spectrometry Coupled with Genetically Encoded Chemical Tags

2020 ◽  
Author(s):  
Feifei Jia ◽  
Jie Wang ◽  
Yanyan Zhang ◽  
Qun Luo ◽  
Luyu Qi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Single Cells ◽  
Time Of Flight ◽  
E Coli ◽  
Tof Sims ◽  
Ion Mass Spectrometry ◽  
Chemical Tags ◽  
Secondary Ion

<p></p><p><i>In situ</i> visualization of proteins of interest at single cell level is attractive in cell biology, molecular biology and biomedicine, which usually involves photon, electron or X-ray based imaging methods. Herein, we report an optics-free strategy that images a specific protein in single cells by time of flight-secondary ion mass spectrometry (ToF-SIMS) following genetic incorporation of fluorine-containing unnatural amino acids as a chemical tag into the protein via genetic code expansion technique. The method was developed and validated by imaging GFP in E. coli and human HeLa cancer cells, and then utilized to visualize the distribution of chemotaxis protein CheA in E. coli cells and the interaction between high mobility group box 1 protein and cisplatin damaged DNA in HeLa cells. The present work highlights the power of ToF-SIMS imaging combined with genetically encoded chemical tags for <i>in situ </i>visualization of proteins of interest as well as the interactions between proteins and drugs or drug damaged DNA in single cells.</p><p></p>

Visualizing mass transport in desorption electrospray ionization using time-of-flight secondary ion mass spectrometry: a look at the geometric configuration of the spray

The Analyst ◽  
2014 ◽  
Vol 139 (22) ◽  
pp. 5868-5878 ◽  
Author(s):  
Shin Muramoto
Keyword(s):  
Mass Spectrometry ◽  
Mass Transport ◽  
Electrospray Ionization ◽  
Secondary Ion Mass Spectrometry ◽  
Time Of Flight ◽  
Desorption Electrospray Ionization ◽  
Geometric Configuration ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

The desorption profile of analyte molecules desorbed by desorption electrospray ionization was imaged and characterized using time-of-flight secondary ion mass spectrometry.

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