scholarly journals Reprogramming efficiency and quality of induced Pluripotent Stem Cells (iPSCs) generated from muscle-derived fibroblasts of mdx mice at different ages

PLoS Currents ◽  
2011 ◽  
Vol 3 ◽  
pp. RRN1274 ◽  
Author(s):  
Bo Wang ◽  
Yuko Miyagoe-Suzuki ◽  
Erica Yada ◽  
Naoki Ito ◽  
Takashi Nishiyama ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Mdx Mice ◽  
Reprogramming Efficiency ◽  
Different Ages ◽  
Induced Pluripotent

scholarly journals Proteins Reprogramming: Present and Future

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Yang Yang ◽  
Bin Liu ◽  
Jianwen Dong ◽  
Liangming Zhang ◽  
Mao Pang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Chemical Compounds ◽  
Insertion Mutation ◽  
Potential Safety ◽  
Reprogramming Efficiency ◽  
Immunological Rejection ◽  
Clinical Interest ◽  
Induced Pluripotent

Induced pluripotent stem cells (iPSCs) are of great clinical interest for they are derived from one’s own somatic cells and have the potential of committed differentiation without immunological rejection after autografting. However, the use of viral and other modified vectors may still cause tumorigenesis due to chromosome insertion mutation, leading to limited practical use. iPSCs generated by reprogramming proteins overcome the potential safety risk and complicated manipulation procedures, thus they own better application prospective, yet some technical difficulties need to be studied and resolved, for instance, low reprogramming efficiency, unclear transduction, and reprogramming mechanism. In this paper, we summarize the current progress of proteins reprogramming technology for generation of iPSCs and discuss the promising efficiency-improved reprogramming methods by proteins plus other kinds of chemical compounds.

Melatonin improves reprogramming efficiency and proliferation of bovine-induced pluripotent stem cells

2016 ◽  
Vol 61 (2) ◽  
pp. 154-167 ◽  
Author(s):  
Chunyu Bai ◽  
Xiangchen Li ◽  
Yuhua Gao ◽  
Ziao Yuan ◽  
Pengfei Hu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Reprogramming Efficiency ◽  
Induced Pluripotent

Culture under low physiological oxygen conditions improves the stemness and quality of induced pluripotent stem cells

2013 ◽  
Vol 228 (11) ◽  
pp. 2159-2166 ◽  
Author(s):  
Chao-Wan Guo ◽  
Miho Kawakatsu ◽  
Marie Idemitsu ◽  
Yoshishige Urata ◽  
Shinji Goto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Oxygen Conditions ◽  
Induced Pluripotent

scholarly journals Technological Progress in Generation of Induced Pluripotent Stem Cells for Clinical Applications

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Seung-Ick Oh ◽  
Chang Kyu Lee ◽  
Kyung Jin Cho ◽  
Kyung-Ok Lee ◽  
Ssang-Goo Cho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Chromosomal Abnormalities ◽  
Patient Specific ◽  
Current State ◽  
Reprogramming Efficiency ◽  
Induced Pluripotent ◽  
Viral Dna Integration ◽  
Medical Interest

Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is achieved by viral-mediated transduction of defined transcription factors. Generation of iPSCs is of great medical interest as they have the potential to be a source of patient-specific cells. For the eventual goal of clinical application, it is necessary to overcome the limitations of low reprogramming efficiency and chromosomal abnormalities due to viral DNA integration. In this paper, we summarize the current state of reprogramming technology for generation of iPSCs and also discuss potential approaches to the development of safe iPSCs for personalized cell-based replacement therapy.

scholarly journals Cellular Ontogeny and Hierarchy Influence the Reprogramming Efficiency of Human B Cells into Induced Pluripotent Stem Cells

Stem Cells ◽  
2016 ◽  
Vol 34 (3) ◽  
pp. 581-587 ◽  
Author(s):  
Álvaro Muñoz-López ◽  
Eddy. H.J. van Roon ◽  
Damià Romero-Moya ◽  
Belén López-Millan ◽  
Ronald W. Stam ◽  
...  
Keyword(s):  
Stem Cells ◽  
B Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human B Cells ◽  
Reprogramming Efficiency ◽  
Induced Pluripotent

scholarly journals Factor-Reduced Human Induced Pluripotent Stem Cells Efficiently Differentiate into Neurons Independent of the Number of Reprogramming Factors

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Andreas Hermann ◽  
Jeong Beom Kim ◽  
Sumitra Srimasorn ◽  
Holm Zaehres ◽  
Peter Reinhardt ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Neuronal Differentiation ◽  
Pluripotent Stem Cells ◽  
Human Cells ◽  
Future Application ◽  
Cell Replacement ◽  
Reprogramming Efficiency ◽  
Reprogramming Factors ◽  
Induced Pluripotent

Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) by overexpression of the transcription factors OCT4, SOX2, KLF4, and c-Myc holds great promise for the development of personalized cell replacement therapies. In an attempt to minimize the risk of chromosomal disruption and to simplify reprogramming, several studies demonstrated that a reduced set of reprogramming factors is sufficient to generate iPSC. We recently showed that a reduction of reprogramming factors in murine cells not only reduces reprogramming efficiency but also may worsen subsequent differentiation. To prove whether this is also true for human cells, we compared the efficiency of neuronal differentiation of iPSC generated from fetal human neural stem cells with either one (OCT4;hiPSC1F-NSC) or two (OCT4, KLF4;hiPSC2F-NSC) reprogramming factors with iPSC produced from human fibroblasts using three (hiPSC3F-FIB) or four reprogramming factors (hiPSC4F-FIB). After four weeks of coculture with PA6 stromal cells, neuronal differentiation ofhiPSC1F-NSCandhiPSC2F-NSCwas as efficient asiPSC3F-FIBoriPSC4F-FIB. We conclude that a reduction of reprogramming factors in human cells does reduce reprogramming efficiency but does not alter subsequent differentiation into neural lineages. This is of importance for the development of future application of iPSC in cell replacement therapies.

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