scholarly journals Genetic Characterization of the Gypsy Moth from China (Lepidoptera, Lymantriidae) Using Inter Simple Sequence Repeats Markers

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e73017 ◽  
Author(s):  
Fang Chen ◽  
Juan Shi ◽  
You-qing Luo ◽  
Shuang-yan Sun ◽  
Min Pu
2017 ◽  
Vol 52 (10) ◽  
pp. 849-860 ◽  
Author(s):  
Kenia Gracielle da Fonseca ◽  
Fábio Gelape Faleiro ◽  
Nilton Tadeu Vilela Junqueira ◽  
Mariana Barth ◽  
Nelson Pires Feldberg

Abstract: The objective of this work was to validate the morphoagronomic descriptors used in the protection processes of plant cultivars in Brazil, by characterizing six cultivars of ornamental passion fruit. The BRS Rubiflora, BRS Rosea Púrpura, BRS Céu do Cerrado, BRS Roseflora, BRS Estrela do Cerrado, and BRS Pérola do Cerrado cultivars, 33 morphoagronomic descriptors, and two molecular markers were used. The categorical morphoagronomic descriptors were analyzed by frequency distribution and multivariate analyses. The quantitative morphoagronomic descriptors were subjected to the analysis of variance and to the comparison of the means of each cultivar. The random amplified polymorphic DNA (RAPD) and the inter-simple sequence repeats (ISSR) markers were used for molecular analysis. A high-validation rate was observed for the morphoagronomic descriptors used in the protection of plant cultivars. The analyses of variance showed significant differences between the quantitative descriptors, and the molecular markers confirmed the genetic differences among the cultivars. There was a high correlation between the calculated distances based on the categorical morphoagronomic descriptors and molecular markers. The morphoagronomic descriptors and molecular markers are useful and complementary for the characterization and differentiation of cultivars.


2008 ◽  
Vol 423 (1) ◽  
pp. 337-341 ◽  
Author(s):  
M. V. Zagoskin ◽  
A. K. Grishanin ◽  
A. L. Korolev ◽  
M. V. Palenko ◽  
D. V. Mukha

2009 ◽  
Vol 32 (1) ◽  
pp. 104-110 ◽  
Author(s):  
Marcos V. B. M. Siqueira ◽  
Jurema R. Queiroz-Silva ◽  
Eduardo A. Bressan ◽  
Aline Borges ◽  
Kayo J. C. Pereira ◽  
...  

2019 ◽  
Vol 20 (2) ◽  
pp. 387-392 ◽  
Author(s):  
VIET THE HO ◽  
HUU TRIET LE ◽  
THANH AN NGUYEN

Ho VT, Le HT, Nguyen TA. 2019. Genetic characterization of Gac (Momordica cochinchinesis) accessions in Southern Vietnam by ISSR markers. Biodiversitas 20: 387-392. Gac (Momordica cochinchinesis) plays important roles in Vietnamese cuisine and medicine. It has high nutrient and medicinal values. Demand of gac fruit as ingredient for food processing and raw material for health industry requires accelerated gac cultivation. However, gac quality is variable due to the uncertain available germplasm and its management as breeding stock. Identification of gac cultivars and landraces in Vietnam is mainly based on personal experience relying on morphological traits, thus the conservation and breeding have low impact on productivity and quality of product. Recently, the development of molecular markers has been useful in identifying specific plant(s). In this study, genetic richness and relativeness of 14 gac accessions collected from different provinces in Southern Vietnam were evaluated by using 10 Inter-Simple Sequence Repeats (ISSR) markers. Results revealed large variation in genetic background of studied gac accessions. Seven DNA-based markers with potential to differentiate gac genotypes were recorded. The results provide molecular biological information for classification, identification plant origins, breeding and conserving programs of gac in Vietnam.


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