scholarly journals Genetic characterization of Iranian safflower (Carthamus tinctorius) using inter simple sequence repeats (ISSR) markers

2013 ◽  
Vol 19 (2) ◽  
pp. 239-243 ◽  
Author(s):  
Bahman Panahi ◽  
Mahmoud Ghorbanzadeh Neghab
Keyword(s):  
Simple Sequence Repeats ◽  
Genetic Characterization ◽  
Carthamus Tinctorius ◽  
Issr Markers ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

scholarly journals Morphoagronomic and molecular characterization of ornamental passion fruit cultivars

2017 ◽  
Vol 52 (10) ◽  
pp. 849-860 ◽  
Author(s):  
Kenia Gracielle da Fonseca ◽  
Fábio Gelape Faleiro ◽  
Nilton Tadeu Vilela Junqueira ◽  
Mariana Barth ◽  
Nelson Pires Feldberg
Keyword(s):  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Multivariate Analyses ◽  
Random Amplified Polymorphic Dna ◽  
Issr Markers ◽  
Passion Fruit ◽  
Validation Rate ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

Abstract: The objective of this work was to validate the morphoagronomic descriptors used in the protection processes of plant cultivars in Brazil, by characterizing six cultivars of ornamental passion fruit. The BRS Rubiflora, BRS Rosea Púrpura, BRS Céu do Cerrado, BRS Roseflora, BRS Estrela do Cerrado, and BRS Pérola do Cerrado cultivars, 33 morphoagronomic descriptors, and two molecular markers were used. The categorical morphoagronomic descriptors were analyzed by frequency distribution and multivariate analyses. The quantitative morphoagronomic descriptors were subjected to the analysis of variance and to the comparison of the means of each cultivar. The random amplified polymorphic DNA (RAPD) and the inter-simple sequence repeats (ISSR) markers were used for molecular analysis. A high-validation rate was observed for the morphoagronomic descriptors used in the protection of plant cultivars. The analyses of variance showed significant differences between the quantitative descriptors, and the molecular markers confirmed the genetic differences among the cultivars. There was a high correlation between the calculated distances based on the categorical morphoagronomic descriptors and molecular markers. The morphoagronomic descriptors and molecular markers are useful and complementary for the characterization and differentiation of cultivars.

scholarly journals Genetic characterization of Gac (Momordica cochinchinesis) accessions in Southern Vietnam by ISSR markers

2019 ◽  
Vol 20 (2) ◽  
pp. 387-392 ◽  
Author(s):  
VIET THE HO ◽  
HUU TRIET LE ◽  
THANH AN NGUYEN
Keyword(s):  
Genetic Characterization ◽  
Issr Markers ◽  
Raw Material ◽  
Biological Information ◽  
Southern Vietnam ◽  
Inter Simple Sequence Repeats ◽  
High Nutrient ◽  
Simple Sequence

Ho VT, Le HT, Nguyen TA. 2019. Genetic characterization of Gac (Momordica cochinchinesis) accessions in Southern Vietnam by ISSR markers. Biodiversitas 20: 387-392. Gac (Momordica cochinchinesis) plays important roles in Vietnamese cuisine and medicine. It has high nutrient and medicinal values. Demand of gac fruit as ingredient for food processing and raw material for health industry requires accelerated gac cultivation. However, gac quality is variable due to the uncertain available germplasm and its management as breeding stock. Identification of gac cultivars and landraces in Vietnam is mainly based on personal experience relying on morphological traits, thus the conservation and breeding have low impact on productivity and quality of product. Recently, the development of molecular markers has been useful in identifying specific plant(s). In this study, genetic richness and relativeness of 14 gac accessions collected from different provinces in Southern Vietnam were evaluated by using 10 Inter-Simple Sequence Repeats (ISSR) markers. Results revealed large variation in genetic background of studied gac accessions. Seven DNA-based markers with potential to differentiate gac genotypes were recorded. The results provide molecular biological information for classification, identification plant origins, breeding and conserving programs of gac in Vietnam.

Characterization of Italian populations ofLoliumspp. resistant and susceptible to diclofop by inter simple sequence repeat

Weed Science ◽  
2004 ◽  
Vol 52 (4) ◽  
pp. 554-563 ◽  
Author(s):  
Giovanni Dinelli ◽  
Alessandra Bonetti ◽  
Ilaria Marotti ◽  
Maurizio Minelli ◽  
Pietro Catizone
Keyword(s):  
Potential Role ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Intergeneric Hybridization ◽  
Banding Patterns ◽  
Inter Simple Sequence Repeats ◽  
Range Of Variation ◽  
Simple Sequence

Three ItalianLoliumweed populations, one susceptible and two resistant to diclofop, were characterized by the technique of inter simple sequence repeats (ISSR). The goal of this study was to taxonomically identify theseLoliumpopulations as well as to evaluate evidence for introgression of ISSR fragments fromFestucaand the potential role of this introgression in the diclofop response. ISSR analysis confirmed the genomic background of the weed populations to be consistent with that ofLolium. However, the great range of variation in ISSR banding patterns highlighted that the three ryegrass accessions are mixed populations made up of individuals resulting presumably from intrageneric and intergeneric hybridization in theLolium–Festucacomplex. TwoFestucagenus-discriminating and 20Festucaspecies-discriminating ISSR markers were screened among all the three ryegrass populations. The resistant Tuscania population carried the highest percentage ofFestucagenome (16.8%) followed by the resistant Roma (13.6%) and susceptible Vetralla (7.6%) populations. On the basis of these data some influence ofFestucagenome in diclofop resistance levels of studied ryegrass populations could be hypothesized.

scholarly journals Characterization of Aegilops kotschyi Boiss. x Triticum aestivum L. hybrid lines

2014 ◽  
Vol 66 (4) ◽  
pp. 109-120 ◽  
Author(s):  
Roman Prażak ◽  
Edyta Paczos-Grzęda
Keyword(s):  
Triticum Aestivum ◽  
Zinc Content ◽  
Triticum Aestivum L ◽  
Issr Markers ◽  
Inter Simple Sequence Repeats ◽  
Iron And Zinc ◽  
Germplasm Analysis ◽  
Hybrid Lines ◽  
Simple Sequence

A study of four F5 and one BC1F1 <em>Aegilops kotschyi </em>Boiss. x <em>Triticum aestivum </em>L. hybrid lines was conducted to determine their quantitative morphological and qualitative features as well as a molecular investigation was carried out. Observations of ten quantitative traits showed that the F5 hybrid lines exhibited intermediate values between <em>Ae. kotschyi </em>Boiss. and <em>T. aestivum </em>L., or had similar traits to one of the parents. These hybrid lines had a significantly lower number and weight of grains per main spike, main spike fertility and 1000-grain weight than <em>T. aestivum </em>L. cv. ‘Rusałka’. The BC1F1 hybrid line was characterized by wheat-like fertility and phenotype. The F5 hybrid lines were characterized by much higher variability of the analysed morphological traits than <em>T. aestivum </em>L. cv. ‘Rusałka’. Grains of the hybrid lines had higher protein and micronutrient (iron and zinc) content than wheat grains. The presence of DNA fragments specific to <em>Ae. kotschyi </em>Boiss. in the genotypes of the hybrid lines was confirmed by seven ISSR (Inter Simple Sequence Repeats) molecular markers. Two ISSR markers – ISSR23690 and ISSR33650 – were the most effective for germplasm analysis of the hybrid lines. The analysed lines can become a source material for improvement of common wheat <em>T. aestivum </em>L. in crossing programs.

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