scholarly journals Bacterial Communities Associated with Host-Adapted Populations of Pea Aphids Revealed by Deep Sequencing of 16S Ribosomal DNA

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0120664 ◽  
Author(s):  
Jean-Pierre Gauthier ◽  
Yannick Outreman ◽  
Lucie Mieuzet ◽  
Jean-Christophe Simon
2001 ◽  
Vol 67 (4) ◽  
pp. 1935-1939 ◽  
Author(s):  
Christopher W. Kaplan ◽  
Johanna C. Astaire ◽  
Mary Ellen Sanders ◽  
Bandaru S. Reddy ◽  
Christopher L. Kitts

ABSTRACT 16S ribosomal DNA terminal restriction fragment patterns from rat fecal samples were analyzed to track the dynamics ofLactobacillus acidophilus NCFM and discern bacterial populations that changed during feeding with NCFM. Lactobacillus johnsonii and Ruminococcus flavefaciens were tentatively identified as such bacterial populations. The presence ofL. johnsonii was confirmed by isolation from feces.


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Pilar Garcia-Jimenez ◽  
Marina Carrasco-Acosta ◽  
Sascha Hettmann

This work analyzes bacterial communities present in evaporation ponds of solar salterns of Gran Canaria and reveals specific organisms through molecular techniques. Solar salterns are protected areas in Canary Islands where salt is produced from sea water by solar and wind powered evaporation. Salt was an important product for ancient islanders who used it for a broad field of purposes, but also has a great importance in recent time for its implications in the island economy.Based on amplifications with specific primers for 16S ribosomal DNA (16S rDNA) and subsequent nested-PCR approaches, different amplicons were obtained, and analyzed in silico. A taxonomic classification was carried out through phylogenetic trees.Results revealed different bacteria according to the evaporation grade of crystallizer ponds in saline works.  It is worthstanding the presence of the genus Halobacterium in all crystallizer ponds. This opens an interesting framework for further studies and continuative molecular characterization approaches of bacterial communities in solar salterns of Gran Canaria.


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