scholarly journals Identification, Heterologous Expression, and Functional Characterization of Bacillus subtilis YutF, a HAD Superfamily 5'-Nucleotidase with Broad Substrate Specificity

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0167580 ◽  
Author(s):  
Natalia P. Zakataeva ◽  
Dmitriy V. Romanenkov ◽  
Yuliya R. Yusupova ◽  
Victoria S. Skripnikova ◽  
Takayuki Asahara ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Substrate Specificity ◽  
Heterologous Expression ◽  
Functional Characterization ◽  
Broad Substrate Specificity ◽  
Had Superfamily

scholarly journals Structural and Functional Characterization of a Lytic Polysaccharide Monooxygenase with Broad Substrate Specificity

2015 ◽  
Vol 290 (38) ◽  
pp. 22955-22969 ◽  
Author(s):  
Anna S. Borisova ◽  
Trine Isaksen ◽  
Maria Dimarogona ◽  
Abhishek A. Kognole ◽  
Geir Mathiesen ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Functional Characterization ◽  
Lytic Polysaccharide Monooxygenase ◽  
Broad Substrate Specificity ◽  
Polysaccharide Monooxygenase

scholarly journals Molecular cloning, heterologous expression, and functional characterization of a cellulolytic enzyme (Cel PRII) from buffalo rumen metagenome

3 Biotech ◽  
2017 ◽  
Vol 7 (4) ◽  
Author(s):  
Ravi K. Shah ◽  
Amrutlal K. Patel ◽  
Deepti M. Davla ◽  
Ishan K. Parikh ◽  
Ramalingam B. Subramanian ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Molecular Cloning ◽  
Functional Characterization ◽  
Cellulolytic Enzyme ◽  
Rumen Metagenome ◽  
Buffalo Rumen

Heterologous expression of an agarase gene in Bacillus subtilis, and characterization of the agarase

2018 ◽  
Vol 120 ◽  
pp. 657-664 ◽  
Author(s):  
Xin Cui ◽  
Yuechen Jiang ◽  
Liuyi Chang ◽  
Lei Meng ◽  
Junhong Yu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Heterologous Expression

scholarly journals Functional and structural characterization of IdnL7, an adenylation enzyme involved in incednine biosynthesis

2019 ◽  
Vol 75 (4) ◽  
pp. 299-306
Author(s):  
Jolanta Cieślak ◽  
Akimasa Miyanaga ◽  
Makoto Takaishi ◽  
Fumitaka Kudo ◽  
Tadashi Eguchi
Keyword(s):  
Amino Acids ◽  
Substrate Specificity ◽  
Natural Product ◽  
Structural Characterization ◽  
Biochemical Analysis ◽  
Natural Product Biosynthesis ◽  
Broad Substrate Specificity ◽  
Selective Incorporation ◽  
Polyketide Antibiotic

Adenylation enzymes play an important role in the selective incorporation of the cognate carboxylate substrates in natural product biosynthesis. Here, the biochemical and structural characterization of the adenylation enzyme IdnL7, which is involved in the biosynthesis of the macrolactam polyketide antibiotic incednine, is reported. Biochemical analysis showed that IdnL7 selects and activates several small amino acids. The structure of IdnL7 in complex with an L-alanyl-adenylate intermediate mimic, 5′-O-[N-(L-alanyl)sulfamoyl]adenosine, was determined at 2.1 Å resolution. The structure of IdnL7 explains the broad substrate specificity of IdnL7 towards small L-amino acids.

Cloning, heterologous expression and characterization of a novel streptomyces trypsin in Bacillus subtilis SCK6

2020 ◽  
Vol 147 ◽  
pp. 890-897 ◽  
Author(s):  
Zhikuan Wang ◽  
Xiaoguang Li ◽  
Jiewei Tian ◽  
Yiwen Chu ◽  
Yongqiang Tian
Keyword(s):  
Bacillus Subtilis ◽  
Heterologous Expression

scholarly journals Heterologous expression and functional characterization of a GH10 endoxylanase from Aspergillus fumigatus var. niveus with potential biotechnological application

2019 ◽  
Vol 24 ◽  
pp. e00382 ◽  
Author(s):  
Josman Velasco ◽  
Bianca Oliva ◽  
Evandro José Mulinari ◽  
Leidy Patricia Quintero ◽  
Awana da Silva Lima ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Aspergillus Fumigatus ◽  
Functional Characterization ◽  
Biotechnological Application

scholarly journals Heterologous Expression and Functional Characterization of Novel CYP2C9 Variants Identified in the Alaska Native People

2020 ◽  
Vol 374 (2) ◽  
pp. 233-240
Author(s):  
Matthew G. McDonald ◽  
Lindsay M. Henderson ◽  
Sutapa Ray ◽  
Catherine K. Yeung ◽  
Amanda L. Johnson ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Alaska Native ◽  
Functional Characterization ◽  
Native People
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