Adiposity QTL Adip20 decomposes into at least four loci when dissected using congenic strains

Both linkage and use of congenic strains have shown that a chromosome region near the gene for the Na-K-ATPase α1-subunit ( Atp1a1) contained a quantitative trait locus (QTL) for blood pressure (BP). Currently, two congenic strains, designated S.M5 and S.M6, were made by replacing a segment of the Dahl salt-sensitive SS/Jr (S) rat by the homologous region of the Milan normotensive rat (MNS). In S.M5, the gene for Atp1a1 is from the MNS strain; whereas in S.M6, Atp1a1 is from the S strain. The baseline activity of the α1-Na-K-ATPase and its stoichiometry were evaluated by an assay of ouabain-sensitive inwardly and outwardly directed 86Rb and 22Na fluxes in erythrocytes. The two congenic strains showed a similar BP, but both had a BP lower than that of S rats ( P < 0.0001). Neither the α1-Na-K-ATPase activity nor its stoichiometry was affected by the substitution of the Atp1a1 alleles of S by those of MNS. Thus the BP-lowering effects observed in S.M5 and S.M6 could not be attributed to the α1-Na-K-ATPase activity or its stoichiometry. Atp1a1 is not supported as a candidate to be a BP QTL.

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Genome-wide isolation of growth and obesity QTL using mouse speed congenic strains

BMC Genomics ◽

10.1186/1471-2164-7-102 ◽

2006 ◽

Vol 7 (1) ◽

Cited By ~ 23

Author(s):

Charles R Farber ◽

Pablo M Corva ◽

Juan F Medrano

Keyword(s):

Congenic Strains ◽

Genome Wide

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Olfactory discrimination of urinary odortypes from congenic strains (DBA/1Bg and DBA1.C57BL10-YBg) of mice differing in their Y chromosomes

Behavior Genetics ◽

10.1007/bf01082463 ◽

1993 ◽

Vol 23 (3) ◽

pp. 251-255 ◽

Cited By ~ 13

Author(s):

Edward Monahan ◽

Kunio Yamazaki ◽

Gary K. Beauchamp ◽

Stephen C. Maxson

Keyword(s):

Olfactory Discrimination ◽

Congenic Strains ◽

Y Chromosomes

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Quantitative trait locus for seizure susceptibility on mouse chromosome 5 confirmed with reciprocal congenic strains

Physiological Genomics ◽

10.1152/physiolgenomics.00123.2007 ◽

2007 ◽

Vol 31 (3) ◽

pp. 458-462 ◽

Cited By ~ 14

Author(s):

Thomas N. Ferraro ◽

George G. Smith ◽

Candice L. Schwebel ◽

Falk W. Lohoff ◽

Patrick Furlong ◽

...

Keyword(s):

Quantitative Trait Locus ◽

Quantitative Trait ◽

Genetic Background ◽

Seizure Threshold ◽

Maximal Electroshock ◽

Seizure Susceptibility ◽

Chromosome 5 ◽

Congenic Strains ◽

Combining Data ◽

Trait Locus

Multiple quantitative trait locus (QTL) mapping studies designed to localize seizure susceptibility genes in C57BL/6 (B6, seizure resistant) and DBA/2 (D2, seizure susceptible) mice have detected a significant effect originating from midchromosome 5. To confirm the presence and refine the position of the chromosome 5 QTL for maximal electroshock seizure threshold (MEST), reciprocal congenic strains between B6 and D2 mice were created by a DNA marker-assisted backcross breeding strategy and studied with respect to changes in MEST. A genomic interval delimited by marker D5Mit75 (proximal to the acromere) and D5Mit403 (distal to the acromere) was introgressed for 10 generations. A set of chromosome 5 congenic strains produced by an independent laboratory was also studied. Comparison of MEST between congenic and control (parental genetic background) mice indicates that genes influencing this trait were captured in all strains. Thus, mice from strains having D2 alleles from chromosome 5 on a B6 genetic background exhibit significantly lower MEST compared with control littermates, whereas congenic mice harboring B6 chromosome 5 alleles on a D2 genetic background exhibit significantly higher MEST compared with control littermates. Combining data from all congenic strains, we conclude that the gene(s) underlying the chromosome 5 QTL for MEST resides in the interval between D5Mit108 (26 cM) and D5Mit278 (61 cM). Generation of interval-specific congenic strains from the primary congenic strains described here may be used to achieve high-resolution mapping of the chromosome 5 gene(s) that contributes to the large difference in seizure susceptibility between B6 and D2 mice.

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