Computational identification of tissue-specific transcription factor cooperation in ten cattle tissues

LFB1 (HNF1) is a tissue-specific transcription factor found in the livers, stomachs, intestines, and kidneys of vertebrates. By analyzing the promoter of the Xenopus LFB1 gene, we identified potential autoregulation by LFB1 and regulation by HNF4, a transcription factor with a tissue distribution similar to that of LFB1. Injection of LFB1 promoter-chloramphenicol acetyltransferase constructs into Xenopus eggs revealed embryonic activation that is restricted to the region of the developing larvae expressing endogeneous LFB1. Proper embryonic activation was also observed with a rat LFB1 promoter. Deletion analysis of the Xenopus and rat promoters revealed that in both promoters embryonic activation is absolutely dependnet on the presence of an element that contains CCNCTCTC as the core consensus sequence. Since this element is recognized by the maternal factor OZ-1 previously described by N. Ovsenek, A. M. Zorn, and P. A. Krieg (Development 115:649-655, 1992), we might have identified the main constituents of a hierarchy that leads via LFB1 to the activation of tissue-specific genes during embryogenesis.

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CpG-depleted promoters harbor tissue-specific transcription factor binding signals—implications for motif overrepresentation analyses

Nucleic Acids Research ◽

10.1093/nar/gkp682 ◽

2009 ◽

Vol 37 (19) ◽

pp. 6305-6315 ◽

Cited By ~ 28

Author(s):

Helge G. Roider ◽

Boris Lenhard ◽

Aditi Kanhere ◽

Stefan A. Haas ◽

Martin Vingron

Keyword(s):

Transcription Factor ◽

Transcription Factor Binding ◽

Tissue Specific ◽

Specific Transcription Factor ◽

Factor Binding

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The tissue-specific transcription factor Pit-1 is expressed in the spinal cord of the lancelet, Branchiostoma lanceolatum

Neuroscience Letters ◽

10.1016/s0304-3940(98)00933-1 ◽

1999 ◽

Vol 260 (1) ◽

pp. 25-28 ◽

Cited By ~ 1

Author(s):

Simona Candiani ◽

Mario Pestarino

Keyword(s):

Spinal Cord ◽

Transcription Factor ◽

Tissue Specific ◽

Specific Transcription Factor ◽

Branchiostoma Lanceolatum

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Elements and factors involved in tissue-specific and embryonic expression of the liver transcription factor LFB1 in Xenopus laevis

Molecular and Cellular Biology ◽

10.1128/mcb.13.10.6416-6426.1993 ◽

1993 ◽

Vol 13 (10) ◽

pp. 6416-6426

Author(s):

D Zapp ◽

S Bartkowski ◽

B Holewa ◽

C Zoidl ◽

L Klein-Hitpass ◽

...

Keyword(s):

Transcription Factor ◽

Xenopus Laevis ◽

Tissue Distribution ◽

Consensus Sequence ◽

Deletion Analysis ◽

Maternal Factor ◽

Tissue Specific ◽

Specific Transcription Factor ◽

The Core ◽

Promoter Deletion Analysis

LFB1 (HNF1) is a tissue-specific transcription factor found in the livers, stomachs, intestines, and kidneys of vertebrates. By analyzing the promoter of the Xenopus LFB1 gene, we identified potential autoregulation by LFB1 and regulation by HNF4, a transcription factor with a tissue distribution similar to that of LFB1. Injection of LFB1 promoter-chloramphenicol acetyltransferase constructs into Xenopus eggs revealed embryonic activation that is restricted to the region of the developing larvae expressing endogeneous LFB1. Proper embryonic activation was also observed with a rat LFB1 promoter. Deletion analysis of the Xenopus and rat promoters revealed that in both promoters embryonic activation is absolutely dependnet on the presence of an element that contains CCNCTCTC as the core consensus sequence. Since this element is recognized by the maternal factor OZ-1 previously described by N. Ovsenek, A. M. Zorn, and P. A. Krieg (Development 115:649-655, 1992), we might have identified the main constituents of a hierarchy that leads via LFB1 to the activation of tissue-specific genes during embryogenesis.

Download Full-text