Diversity of phlebotomine sand flies and molecular detection of trypanosomatids in Brumadinho, Minas Gerais, Brazil

Several phlebotomine sand fly species have been regarded as putative or proven vectors of parasites of the genus Leishmania in Brazil, but data for the northeastern region remains incipient. In this study, a total of 600 phlebotomine sand flies were grouped in pools of 10 specimens each and tested by a Leishmania genus-specific PCR and by a PCR targeting Leishmania (Leishmania) infantum. Fourteen out of 60 pools were positive by the genus-specific PCR, being five pools of L. migonei, seven of L. complexa, one of L. sordellii and one of L. naftalekatzi, which correspond to a minimal infection rate of 2.3% (14/600). Our results, associated with their known anthropophily and their abundance, suggest the participation of L. migonei and L. complexa as vectors of Leishmania in northeastern Brazil. Remarkably, this is the first time in this country that the detection of Leishmania DNA in L. sordellii and L. naftalekatzi has been reported, but future studies are necessary to better understand the significance of these findings.

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Ecology of phlebotomine sand flies in an area of leishmaniasis occurrence in the Xakriabá Indigenous Reserve, Minas Gerais, Brazil

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/0037-8682-0474-2018 ◽

2019 ◽

Vol 52 ◽

Cited By ~ 1

Author(s):

André Tetzl Costa ◽

Edelberto Santos Dias ◽

Andreza Geisiane Maia Souza ◽

Fabiana de Oliveira Lara e Silva ◽

George Luiz Lins Machado-Coelho

Keyword(s):

Minas Gerais ◽

Sand Flies ◽

Phlebotomine Sand Flies

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The phlebotomine sand flies fauna in Parque Estadual do Rio Doce, Minas Gerais, Brazil

Parasites & Vectors ◽

10.1186/s13071-015-1227-1 ◽

2015 ◽

Vol 8 (1) ◽

Cited By ~ 3

Author(s):

Cristian Ferreira de Souza ◽

Reginaldo Peçanha Brazil ◽

Paula Dias Bevilacqua ◽

Jose Dilermando Andrade Filho

Keyword(s):

Minas Gerais ◽

Sand Flies ◽

Phlebotomine Sand Flies

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Molecular Detection of Bartonella sp. in Psathyromyia shannoni and Lutzomyia cruciata From Northeastern Mexico

Frontiers in Tropical Diseases ◽

10.3389/fitd.2021.780808 ◽

2021 ◽

Vol 2 ◽

Author(s):

Yokomi N. Lozano-Sardaneta ◽

Nadia Joselyne Soto-Olguín ◽

Jorge J. Rodríguez-Rojas ◽

Sokani Sánchez-Montes ◽

Eduardo A. Rebollar-Téllez ◽

...

Keyword(s):

Dna Sequences ◽

Molecular Detection ◽

Citrate Synthase ◽

Phylogenetic Reconstruction ◽

Sand Fly ◽

Sand Flies ◽

Light Traps ◽

Bartonella Bacilliformis ◽

Phlebotomine Sand Flies ◽

Northeastern Mexico

Phlebotomine sand flies are vectors of Leishmania spp., Bartonella bacilliformis, and several arboviruses worldwide. In Mexico, the presence of Bartonella species is associated sporadically with arthropods and little is known on the diversity of insects that could be incriminated with its transmission. The aim of this study was to perform a molecular detection of Bartonella DNA in sand fly species collected in northeastern Mexico. Sand flies were collected at the states of Nuevo Leon and Tamaulipas from June to August 2010, using 16 light traps per night. Sand fly species were morphologically identified, and for Bartonella detection, we amplified ~378 bp of the citrate synthase gene (gltA). DNA sequences were compared in a phylogenetic reconstruction based on maximum likelihood. A total of 532 specimens from seven sand fly species were morphologically identified, where 11 specimens from Tamaulipas tested positive for the presence of a new lineage of Bartonella sp. associated with Psathyromyia shannoni and Lutzomyia cruciata. This work represents the second record of Bartonella-associated with sand flies outside of the endemic area of Carrion’s disease. More studies are necessary to understand their life cycle, transmission dynamics, and their relationship with sand fly species.

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Similarity of species composition of sand flies (Diptera: Psychodidae) in caves of Quadrilátero Ferrífero, state Minas Gerais, Brasil

10.1101/703231 ◽

2019 ◽

Author(s):

Aldenise Martins Campos ◽

Rodrigo dos Anjos Maia ◽

Débora Capucci ◽

Adriano Pereira Paglia ◽

José Dilermando Andrade Filho

Keyword(s):

Species Composition ◽

Abundant Species ◽

Minas Gerais ◽

Sand Fly ◽

Southeastern Brazil ◽

Lutzomyia Longipalpis ◽

Sand Flies ◽

Dissimilarity Matrix ◽

Phlebotomine Sand Flies ◽

Quadrilátero Ferrífero

AbstractCaves, though seemingly inhospitable due to the lack of light in areas furthest from the cave entrance and the low availability and variety of resources, can harbor many species of vertebrates and invertebrates, including phlebotomine sand flies, the vectors of species of parasites of the genus Leishmania that cause leishmaniasis. This study aimed to evaluate the species composition of sand flies in located caves at Moeda Sul (MS) and Parque Estadual Serra do Rola Moça (PESRM), in southeastern Brazil at region of the Quadrilátero Ferrífero, state of Minas Gerais. The collections of sand flies were performed with automatic light traps. Were used the Non-Metric Multidimensional Scaling based on dissimilarity matrix calculated with the Jaccard índex and Multivariate Analysis of Permutation to evaluate similarity of the species composition of sand flies among entrance, interior, and surroundings environments of each sampled cave at MS and PESRM, and thus to infer biological mechanisms from the patterns of distribution of sand flies among these different cave environments. A total of 375 phlebotomine sand flies were collected from six genera and 14 species. The most abundant species were Evandromyia tupynambai (54.7%), Brumptomyia troglodytes (25.6%), Evandromyia edwardsi, (6.1%) Psathyromyia brasiliensis (4.8%) and Lutzomyia longipalpis (4.3%). In place MS, 30 individuals were collected, 16 inside the MS cave and 14 its surroundings. In place PESRM, five individuals were collected in surroundings of RM38 cave; a total of 190 individuals at RM39 cave (48 in the cave and 142 its surroundings) and 150 individuals at RM40 cave, 42 in the cave and 108 its surroundings. Our results showed rich fauna of sand flies with a species composition of sand flies similar among the entrance, interior, and surroundings environments of each sampled cave, suggesting that both caves and their surroundings are important for the maintenance of sand fly communities.

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