scholarly journals Genetic characterization of a mild isolate of papaya ringspot virus type-P (PRSV-P) and assessment of its cross-protection potential under greenhouse and field conditions

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0241652
Author(s):  
Andres X. Medina-Salguero ◽  
Juan F. Cornejo-Franco ◽  
Sam Grinstead ◽  
Joseph Mowery ◽  
Dimitre Mollov ◽  
...  

A mild isolate of Papaya ringspot virus type-P, abbreviated as PRSV-mild, from Ecuador was sequenced and characterized. The most distinguishing symptom induced by PRSV-mild was gray powder-like leaf patches radiating from secondary veins. In greenhouse experiments, PRSV-mild did not confer durable protection against a severe isolate of the virus (PRSV-sev), obtained from the same field. Furthermore, isolate specific detection in mixed-infected plants showed that PRSV-sev becomes dominant in infections, rendering PRSV-mild undetectable at 90–120 days post superinfection. Virus testing using isolate-specific primers detected PRSV-mild in two out of five surveyed provinces, with 10% and 48% of incidence in Santo Domingo and Los Ríos, respectively. Comparative genomics showed that PRSV-mild lacks two amino acids from the coat protein region, whereas amino acid determinants for asymptomatic phenotypes were not identified. Recombination events were not predicted in the genomes of the Ecuadorean isolates. Phylogenetic analyses placed both PRSV-mild and PRSV-sev in a clade that includes an additional PRSV isolate from Ecuador and others from South America.

2020 ◽  
Author(s):  
Andres X. Medina-Salguero ◽  
Juan F. Cornejo-Franco ◽  
Sam Grinstead ◽  
Joseph Mowery ◽  
Dimitre Mollov ◽  
...  

AbstractA mild isolate of Papaya ringspot virus type-P, abbreviated as PRSV-mild, from Ecuador was sequenced and characterized. The most distinguishing symptom induced by PRSV-mild was gray powder-like leaf spots radiating from secondary veins. In greenhouse experiments, PRSV-mild did not confer durable protection against a severe isolate of the virus (PRSV-sev), obtained from the same field. Furthermore, isolate specific detection in cross-protected plants showed that PRSV-sev becomes dominant in infections, rendering PRSV-mild undetectable at 90 - 120 days post superinfection. Virus testing using isolate-specific primers detected PRSV-mild in two out of five surveyed provinces, with 10% and 48% of incidence in Santo Domingo and Los Ríos, respectively. Comparative genomics showed that PRSV-mild lacks two amino acids from the coat protein region, whereas amino acid determinants for asymptomatic phenotypes were not identified. Recombination events were not predicted in the genomes of the Ecuadorean isolates. Phylogenetic analyses placed both PRSV-mild and PRSV-sev in a clade that includes an additional PRSV isolate from Ecuador and others from South America.


Virus Genes ◽  
2007 ◽  
Vol 35 (2) ◽  
pp. 431-431
Author(s):  
Juan Carlos Noa-Carrazana ◽  
Diego González-de-León ◽  
Laura Silva-Rosales

Virus Genes ◽  
2006 ◽  
Vol 35 (1) ◽  
pp. 109-117 ◽  
Author(s):  
Juan Carlos Noa-Carrazana ◽  
Diego González-de-León ◽  
Laura Silva-Rosales

2019 ◽  
Vol 101 (4) ◽  
pp. 1203-1209
Author(s):  
Asawari Gorane ◽  
Raj Verma ◽  
Archana Naik ◽  
Tukaram Nikam ◽  
Avinash Ade ◽  
...  

2009 ◽  
Vol 18 (6) ◽  
pp. 971-986 ◽  
Author(s):  
Ming-Jen Fan ◽  
Shu Chen ◽  
Yi-Jung Kung ◽  
Ying-Huey Cheng ◽  
Huey-Jiunn Bau ◽  
...  

2002 ◽  
Vol 83 (10) ◽  
pp. 2575-2585 ◽  
Author(s):  
Marion F. Bateson ◽  
Rosemarie E. Lines ◽  
Peter Revill ◽  
Worawan Chaleeprom ◽  
Cuong V. Ha ◽  
...  

The potyvirus Papaya ringspot virus (PRSV) is found throughout the tropics and subtropics. Its P biotype is a devastating pathogen of papaya crops and its W biotype of cucurbits. PRSV-P is thought to arise by mutation from PRSV-W. However, the relative impact of mutation and movement on the structure of PRSV populations is not well characterized. To investigate this, we have determined the coat protein sequences of isolates of both biotypes of PRSV from Vietnam (50), Thailand (13), India (1) and the Philippines (1), and analysed them together with 28 PRSV sequences already published, so that we can better understand the molecular epidemiology and evolution of PRSV. In Thailand, variation was greater among PRSV-W isolates (mean nucleotide divergence 7·6%) than PRSV-P isolates (mean 2·6%), but in Vietnamese populations the P and W biotypes were more but similarly diverse. Phylogenetic analyses of PRSV also involving its closest known relative, Moroccan watermelon mosaic virus, indicate that PRSV may have originated in Asia, particularly in the Indian subcontinent, as PRSV populations there are most diverse and hence have probably been present longest. Our analyses show that mutation, together with local and long-distance movement, contributes to population variation, and also confirms an earlier conclusion that populations of the PRSV-P biotype have evolved on several occasions from PRSV-W populations.


Virus Genes ◽  
2006 ◽  
Vol 35 (1) ◽  
pp. 119-127 ◽  
Author(s):  
Alice Kazuko Inoue-Nagata ◽  
Carolina de Mello Franco ◽  
Darren Patrick Martin ◽  
Jorge Alberto Marques Rezende ◽  
Gleydson Barros Ferreira ◽  
...  

2015 ◽  
Vol 31 (4) ◽  
pp. 1133-1142 ◽  
Author(s):  
Douglas Rodríguez-Martínez ◽  
Antonia dos Reis Figueira ◽  
Priscilla de Sousa Geraldino Duarte ◽  
Suellen Bárbara Ferreira Galvino-Costa ◽  
Justo González Olmedo

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