scholarly journals Tracking Macroprolactin: Use of an Optimized Polyethylene Glycol Precipitation Method More Compatible with the Requirements and Processes of Automated Core Laboratories

2017 ◽  
Vol 1 (6) ◽  
pp. 661-667 ◽  
Author(s):  
Julien Favresse ◽  
Pierre Bastin ◽  
Catherine Fillée ◽  
Francoise Luyckx ◽  
Dominique Maiter ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Precipitation Method ◽  
Polyethylene Glycol Precipitation

scholarly journals Macroenzyme detection by polyethylene glycol precipitation

2003 ◽  
Vol 40 (5) ◽  
pp. 514-520 ◽  
Author(s):  
D Fraser Davidson ◽  
Dawn JM Watson
Keyword(s):  
Enzyme Activity ◽  
Polyethylene Glycol ◽  
Typical Case ◽  
Precipitation Method ◽  
Reference Ranges ◽  
Case Histories ◽  
Additional Test ◽  
Plasma Enzyme ◽  
Diagnostic Confusion ◽  
Polyethylene Glycol Precipitation

Background: The presence of macroenzymes can cause significant diagnostic confusion and their detection can involve relatively cumbersome analytical procedures. Methods: Using a simplified polyethylene glycol precipitation technique and isoenzyme electrophoresis, this report describes the construction of reference ranges of precipitable activity for each of seven commonly measured enzymes in plasma. Results: The proposed reference ranges are reported. Since introducing the protocol, 12 cases of macroenzymaemia have been encountered. Three typical case histories are described in some detail. Conclusions: The polyethylene glycol precipitation method has thus far proved to be a simple and effective additional test for the detection of macroenzymes when the plasma enzyme activity is elevated.

scholarly journals A 2-year survey of the prevalence of enteric viral infections in children compared with contamination in locally-harvested oysters

1992 ◽  
Vol 108 (1) ◽  
pp. 155-163 ◽  
Author(s):  
T. Yamashita ◽  
K. Sakae ◽  
Y. Ishihara ◽  
S. Isomura
Keyword(s):  
Polyethylene Glycol ◽  
Viral Infections ◽  
Enteric Viruses ◽  
Precipitation Method ◽  
Safety Standards ◽  
Aichi Prefecture ◽  
Human Enteric Viruses ◽  
Sick Children ◽  
Polyethylene Glycol Precipitation

SUMMARYWe studied, for two years, the prevalence of indigenous human enteric viruses in wild oysters gathered each month from the bottom of Mikawa Bay, Aichi Prefecture, Japan. Viruses were detected periodically in 9 out of 54 oyster pools prepared by the acid or polyethylene glycol precipitation method although all these 9 pools met current national bacteriological safety standards. Since most of the serotypes of the enteric viruses detected in the oysters were identical with those of viruses isolated from sick children living in the area, it is suggested that contamination of enteric viruses in the oysters would depend on the prevalence of enteric viral infections in the local inhabitants.

scholarly journals Assessment of macroprolactinemia by the polyethylene glycol precipitation method

2020 ◽  
Vol 73 (7-8) ◽  
pp. 229-234
Author(s):  
Stanislava Nikolic ◽  
Romana Mijovic ◽  
Dragana Oluski ◽  
Tanja Ostojic ◽  
Ivana Bajkin
Keyword(s):  
Polyethylene Glycol ◽  
The Other ◽  
Precipitation Method ◽  
Serum Prolactin ◽  
Serum Samples ◽  
Simple Method ◽  
Significant Difference ◽  
Low Incidence ◽  
Polyethylene Glycol Precipitation ◽  
Phosphate Buffered Saline

Introduction. Macroprolactinemia is one of the common causes of hyperprolactinemia, especially in cases where standard routine commercial immunometric assays are used for prolactin level measurement. Two forms of prolactin, inactive dimeric prolactin (50 - 60 kDa) and a low-activity tetramer (molecular weight above 150 kDa), contribute to this condition. The relatively low incidence of symptoms in macroprolactinemia patients has necessitated a relatively simple method to detect large, biologically inactive prolactin molecules, such as polyethylene glycol precipitation method. The aim of this study was to compare the precipitation method using polyethylene glycol dissolved in phosphate buffered saline in relation to polyethylene glycol dissolved in water. Material and Methods. This study included 82 patients who visited the Center of Laboratory Medicine, Clinical center of Vojvodina, to determine serum prolactin levels. The obtained serum samples were divided into two aliquots. The first aliquot was frozen and stored at - 20?C and the other was immediately analyzed. Aqueous solution was added to one aliquot and polyethylene glycol Merck 6000 to the other. All serum and supernatant samples were analyzed using the automated Abbott Architect i2000sr immunoassay. Recovery values were calculated as the ratio of double values of free prolactin and total prolactin concentration, expressed as a percentage. Results. The prolactin concentrations and calculated recovery values were lowest in fresh supernatant prolactin treated with phosphate buffered saline. Statistical analysis showed no significant difference in the calculated recovery values obtained by precipitation of fresh and frozen sera using polyethylene glycol dissolved in phosphate buffered saline (p = 0.893). Conclusion. Precipitation using polyethylene glycol dissolved in phosphate buffered saline is a more reliable method for laboratory detection of macroprolactinemia compared to polyethylene glycol dissolved in water.

scholarly journals Assessment of macroprolactinemia by polyethylene glycol precipitation method

2014 ◽  
Vol 9 (1) ◽  
pp. 25-28 ◽  
Author(s):  
Ana Maia Silva ◽  
Paula Martins da Costa ◽  
Ana Pacheco ◽  
José Carlos Oliveira ◽  
Cláudia Freitas
Keyword(s):  
Polyethylene Glycol ◽  
Precipitation Method ◽  
Polyethylene Glycol Precipitation
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